Effects of Smoking and Vitamin D3 on the Levels of Human Cathelicidin Peptide LL-37

NCT03923218 | Completed DMC

• 1 other identifier: 03/2011-27
• Study Type: observational
• Target: 60
• Locations: 0 countries
• Sites: N/A

Brief Summary

The aims of our study were 1) to evaluate levels of gingival crevicular fluid( GCF) human cathelicidin peptide LL-37 and serum vitamin D3 in smoker and non-smoker patients with chronic periodontitis(CP) 2) to determine whether any correlation between GCF LL-37 and vitamin D3 serum levels exist and 3) to asses the correlation between clinical parameters and biochemical markers

Conditions

Periodontitis

Keywords

Gingival crevicular fluid; Vitamin D; LL-37; Serum; periodontitis

Outcome Measures

Primary Outcomes (2)

• Serum D3 Vitamin levels

  The plasma levels of vitamin D3 were greater in the CP-2 group than in the CP-1 group. But, no statistically significant difference was determined between the CP groups (P\>0.05)

  Baseline

• Gingival Crevicular fluid(GCF) LL-37 levels

  The concentration of LL-37 in GCF was significantly higher in both of the CP groups than in the CTRL group (p\<0.001). Although GCF LL-37 concentration levels were greater in the CP-1 group than in the CP-2 group, the difference did not reach the statistical significance between the CP groups (p\>0.05).

  Baseline

Secondary Outcomes (5)

• Plaque index (PI)

  Baseline

• GCF volume

  Baseline

• gingival index (GI)

  Baseline

• Probing depth (PD)

  Baseline

• Clinical attachment level (CAL)

  Baseline

Study Arms (3)

Smoker patients with chronic periodontitis

Smoker patients with chronic periodontitis

Other: LL-37 levels in Gingival crevicular fluid; Other: Serum Vitamin D3 levels; Other: Clinical parameters

non-smoker patients with chronic periodontitis

non-smoker patients with chronic periodontitis

Other: LL-37 levels in Gingival crevicular fluid; Other: Serum Vitamin D3 levels; Other: Clinical parameters

periodontally healthy patients

periodontally healthy patients

Other: LL-37 levels in Gingival crevicular fluid; Other: Serum Vitamin D3 levels; Other: Clinical parameters

Interventions

LL-37 levels in Gingival crevicular fluid OTHER

Collected gingival crevicular fluid

Smoker patients with chronic periodontitis; non-smoker patients with chronic periodontitis; periodontally healthy patients

Serum Vitamin D3 levels OTHER

Collected serum

Smoker patients with chronic periodontitis; non-smoker patients with chronic periodontitis; periodontally healthy patients

Clinical parameters OTHER

Recorded plague index, gingival index, probing depth, clinical attachment level

Smoker patients with chronic periodontitis; non-smoker patients with chronic periodontitis; periodontally healthy patients

Eligibility Criteria

• Age: 35 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

The volunteers participitating in the study were choosen from among individuals scheduled to undergo either dental treatment or dental check at the Deparment of Perioodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey.

You may qualify if:

• Clinical attachment loss ≥ 5mm
• Probing depth ≥5mm
• Bone loss affecting \>30% of the existing teeth on clinical and radiographic examination
• gingival index (GI) score\> 1
• full-mouth PD was≤ 3mm,
• Gingival index \<1
• there was no indication of Attachment Loss or no radiographic evidence of alveolar bone loss in control group.
• Non-smokers.
• Smoking criteria for groups:
• patients smoked more than 10 cigarettes in a day
• smoking for 3 or more years,

You may not qualify if:

• systemic condition (diabetes, cardiovascular disease, immunologic disorders, hepatitis, e.g.)
• pregnancy, lactation or menopause term,
• antibiotic treatment or non-steroidal anti-inflammatory medications within the last 3 months before the study,
• non-surgical periodontal treatment during the last 6 months before the study,
• treatment with Vitamin D supplementation before the study

Sponsors & Collaborators

• Gazi University: lead

MeSH Terms

Conditions

Periodontitis

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Officials

• Şeyma BOZKURT DOĞAN, Professor

  university

  PRINCIPAL INVESTIGATOR

• Elifcan Kıvrak, PhD

  university

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: April 17, 2019
• First Posted: April 22, 2019
• Study Start: December 1, 2011
• Primary Completion: July 1, 2012
• Study Completion: July 1, 2015
• Last Updated: April 22, 2019

Record last verified: 2019-04