STAT4 in Multiple Sclerosis by PCR and Flow Cytometry

NCT03893344 | Completed

• 1 other identifier: STAT4 gene in MS.
• Study Type: observational
• Target: 104
• Locations: 1 country
• Sites: 1

Brief Summary

1. 1.To determine the level of STAT4 expression in different cases of multiple sclerosis and its relation to disease severity .
2. 2.compare the sensitivity and specificity of STAT4 levels using both PCR and flow cytometry.

Conditions

Multiple Sclerosis

Outcome Measures

Primary Outcomes (1)

• To determine the level of STAT4 expression in different cases of multiple sclerosis

  measuring the level of STAT4 gene in both the control group and different cases of multiple sclerotic and its relation to disease pathogenesis and severity

  baseline

Secondary Outcomes (1)

• compare the sensitivity and specificity of STAT4 levels using both PCR and flow cytometry.

  baseline

Study Arms (2)

Cases

patients with Multiple Sclerosis in different stages diagnosed clinically according to revised McDonald's criteria 2010

Diagnostic Test: detection of STAT4 gene

Control

Healthy peaple

Diagnostic Test: detection of STAT4 gene

Interventions

detection of STAT4 gene DIAGNOSTIC_TEST

Individuals within both groups will be subjected to STAT4 gene polymorphism typing using both PCR and flow cytometry from a peripheral blood sample

Cases; Control

Eligibility Criteria

• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

MS patients, fulfilling the inclusion criteria for the research, will be selected from Assiut University Hospital.

You may qualify if:

• The diagnosis of MS patients will be established clinically from the history according to revised McDonald's criteria 2010.

You may not qualify if:

• Patients proved not to have MS either at first interview or at any time during follow up or with other autoimmune diseases will be ruled out of the study.
• Healthy group with past or present medical history of autoimmune disease or other neurologic condition.

Sponsors & Collaborators

• Assiut University: lead

Study Sites (1)

Assiut University Hospital

Asyut, 71111, Egypt

Location

Related Publications (2)

• Housley WJ, Fernandez SD, Vera K, Murikinati SR, Grutzendler J, Cuerdon N, Glick L, De Jager PL, Mitrovic M, Cotsapas C, Hafler DA. Genetic variants associated with autoimmunity drive NFkappaB signaling and responses to inflammatory stimuli. Sci Transl Med. 2015 Jun 10;7(291):291ra93. doi: 10.1126/scitranslmed.aaa9223.

  PMID: 26062845 BACKGROUND

• Filippi M, Bar-Or A, Piehl F, Preziosa P, Solari A, Vukusic S, Rocca MA. Multiple sclerosis. Nat Rev Dis Primers. 2018 Nov 8;4(1):43. doi: 10.1038/s41572-018-0041-4.

  PMID: 30410033 BACKGROUND

Related Links

• Aberrant STAT phosphorylation signaling in peripheral blood mononuclear cells from multiple sclerosis patients. Journal of Neuroinflammation 15:72
• Association of STAT4 polymorphism with severe renal insufficiency in lupus nephritis. PLoS One.8(12):e84450

MeSH Terms

Conditions

Multiple Sclerosis

Condition Hierarchy (Ancestors)

Demyelinating Autoimmune Diseases, CNS; Autoimmune Diseases of the Nervous System; Nervous System Diseases; Demyelinating Diseases; Autoimmune Diseases; Immune System Diseases

Study Officials

• Ahmed K Mostafa, Professor

  Assiut University

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Lecturer at Assiut University

Study Record Dates

• First Submitted: March 26, 2019
• First Posted: March 28, 2019
• Study Start: September 20, 2019
• Primary Completion: May 20, 2020
• Study Completion: May 22, 2020
• Last Updated: June 9, 2020

Record last verified: 2020-06

Locations

EG (1)