Hemodialysis and Erythrocyte Fatty-Acid Status: a Lipidomics Study

NCT03857984 | Completed

• 1 other identifier: E2/113/08
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

Hypotension with potentially serious consequences for organ perfusion is a common complication in extracorporeal procedures such as hemodialysis / hemofiltration. The exact reasons for this are still insufficiently clarified. Probably periinterventional vasorelaxant released substances play a crucial role in these procedures. These substances could be due to contact of blood cells with the Membrane in the dialyzer / hemofilter arise. In this project the hypothesis will be checked, if EETs / DHETs are released by Erythrocytes during hemodialysis and thus act as potential candidate products for the result of hypotonic phases in the Dialysis. We will determine differences in RBC fatty acids profiling in hemodialysis patients before and after dialysis intervention. Furthermore a matched control group of healthy individuals will be profiled. RBC fatty acids profiling will be achieved by using targeted HPLC-MS mass spectrometry. It is believed that during hemodialysis / HLM / CVVH there is an increase in EETs / DHETs in the serum and in the erythrocytes. It is believed that shear forces play an important role in the release of erythrocyte EETs / DHETs.

Conditions

Fatty Acid Metabolism; Erythrocytes; Lipidomics; Hemodialysis

Outcome Measures

Primary Outcomes (1)

• Coefficient of variation of relative quantities of erythrocyte fatty-acid parameters related to its dynamic during Hemodialysis, using LC-MS / MS.

  EETs / DHETs will be analyzed in the erythrocytes and serum of the recovered blood samples. The determination will made in cooperation with a Berliner Biotech company. This involves liquid chromatography with MS coupling (LC-MS / MS) used. The data analysis is carried out by means of suitable statistical methods to perform erythrocyte fatty-acid status profiling.

  4-5 Months

Study Arms (1)

Erythrocyte Fatty-Acid Status

EXPERIMENTAL

Erythrocyte Fatty-Acid Status Profiling of HD patients are studied before and after a single HD using LC-MS / MS

Other: Blood sampling

Interventions

Blood sampling OTHER

blood sample before and after a single HD

Erythrocyte Fatty-Acid Status

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Patients who have a history of renal failure and undergo hemodialysis / hemofiltration
• Age over 18 years
• Ability to consent
• There is a written consent of the study participant

You may not qualify if:

• chronic illness requiring any medication
• pregnancy
• limitations regarding functioning in the hypoxic chamber
• inability to follow simple instructions relevant or severe abnormalities in medical history, physical examination

Sponsors & Collaborators

• Benjamin Gollasch: lead

Study Sites (1)

Charité University Experimental & Clinical Research Center

Berlin, 13125, Germany

Location

MeSH Terms

Interventions

Blood Specimen Collection

Intervention Hierarchy (Ancestors)

Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Investigative Techniques

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: February 24, 2019
• First Posted: February 28, 2019
• Study Start: May 1, 2018
• Primary Completion: January 1, 2022
• Study Completion: February 1, 2022
• Last Updated: March 10, 2022

Record last verified: 2022-03

Locations

DE (1)