Recovery, Fatigability, and Proteomic Response to Aerobic Exercise Training in Healthy Individuals

NCT03800342 | Completed

• 1 other identifier: VCO2-Proteomics
• Study Type: interventional
• Target: 21
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this protocol is to investigate the role of expired non-metabolic carbon dioxide in the relationship between fatigability and recovery and the response to aerobic exercise training in healthy individuals. Both fatigability and recovery are profoundly influenced by mitochondrial energetics which can be inhibited by ionic by-product accumulation during exercise. Buffering mechanisms of these fatigue-inducing ions releases non-metabolic carbon dioxide (CO2) that can be measured as expired CO2 (VCO2) during cardiopulmonary exercise testing (CPET), however the role of non-metabolic VCO2 in the relationship between fatigability and recovery has yet to be investigated. Furthermore, this study aims to identify the how the patterns of proteins in healthy individuals respond to aerobic exercise training (e.g. stationary cycling) over approximately one month. The underlying mechanisms of recovery after physical activity, including mechanisms or biological pathways that could be highlighted by analysis of proteins in urine, could add to scientific knowledge regarding physical activity tolerance and potential exercise interventions. This knowledge could eventually assist with designing precise and personalized exercise interventions to improve physical activity performance. The investigators hypothesize that 1) non-metabolic CO2 will be at least moderately associated with the inverse relationship between fatigability and recovery; and 2) highly active adults, compared to sedentary individuals, will exhibit differential proteomic patterns in response to an initial acute bout and subsequent repeated bouts of aerobic exercise.

Conditions

Adult; Fatigue

Keywords

recovery; proteomics; cardiorespiratory fitness; exercise; aerobic exercise training

Outcome Measures

Primary Outcomes (1)

• Non-metabolic VCO2

  Correlate measures of non-metabolic carbon dioxide (as measured by the contribution of total expired non-metabolic VCO2) with the correlative relationship between fatigability (as measured by total time during an endurance CPET and on-kinetics during a constant square-wave CPET) and recovery (as measured by VO2 and VCO2 following maximal and submaximal CPET). Compare changes in measures of non-metabolic carbon dioxide (as measured by the contribution of total expired non-metabolic VCO2) and changes in oxygen consumption (as measured by VO2) pre and post exercise training.

  pre and post 5 week (4 training sessions per week, 17 total sessions) aerobic exercise training protocol

Secondary Outcomes (1)

• Urinary proteome

  This outcome will be assessed at 10 time points per participant: each morning of visits 3,4,7,8,11,12,15,16,19, and 20. Data will be collected during these 5 weeks and at post-testing occurring the week following the end of training.

Study Arms (1)

Healthy

EXPERIMENTAL

Healthy individuals will participate in two separate days of cardiopulmonary exercise testing (CPET) (separated by a minimum of two, maximum of 7 days apart) prior to starting the aerobic exercise training program (AET). Individuals will then complete a 4-5 week (4x/week x 17 sessions) continuous, high-intensity AET. Each training session will consist of cycling for 3-5 minutes to warm-up, 45 minutes at 70% of heart rate reserve (HRR-determined from pre-training CPET), and 5-10 minutes to cool down. Following the AET, individuals will repeat the two separate days of CPET performed pre-training.

Other: Aerobic Exercise Training

Interventions

Aerobic Exercise Training OTHER

see arm/group description

Healthy

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• age 18-60
• body mass index \> 19 to \<35 kg/m2
• able to pedal leg cycle ergometer
• able to comprehend and speak English

You may not qualify if:

• diabetes mellitus
• significant pulmonary dysfunction (eg. chronic obstructive lung disease; interstitial lung disease)
• hypertension
• anemia
• stroke
• cancer (other than melanoma)
• cardiac, pulmonary, thyroid, autoimmune, musculoskeletal, neurological, metabolic bone, mitochondrial, hepatic, renal, and/or psychiatric disease
• abnormal blood lipids
• active substance abuse or cognitive impairment
• chronic infection requiring antiviral or antibiotic treatment
• taking any medications that may limit exercise capacity or the ability to adapt to aerobic exercise training
• previously or currently on anticoagulant therapy or therapeutic hormone replacement/supplementation (excluding birth control)
• pregnant
• smoking

Sponsors & Collaborators

• George Mason University: lead

Study Sites (1)

George Mason University

Fairfax, Virginia, 22030, United States

Location

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  BACKGROUND

MeSH Terms

Conditions

Fatigue; Motor Activity

Condition Hierarchy (Ancestors)

Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Behavior

Study Officials

• Andrew A Guccione, PT, PhD, DPT

  George Mason University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: One arm, a single group of healthy individuals, will perform cardiopulmonary exercise testing pre and post an aerobic exercise training program.

Study Record Dates

• First Submitted: December 18, 2018
• First Posted: January 11, 2019
• Study Start: January 22, 2019
• Primary Completion: April 24, 2019
• Study Completion: April 24, 2019
• Last Updated: May 16, 2019

Record last verified: 2019-05

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)