NCT03779022

Brief Summary

MicroRNA (miRNA) is a type of endogenous non-coding RNA. They are responsible for post-transcriptional regulation and participate in many vital biological processes. Expression profiling has shown that miRNAs can distinguish between normal breast and tumor tissues. In recent years, circulating miRNAs have become promising biomarkers based on their stability and their non-invasive testing and feasibility in clinical practices.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
100

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Nov 2015

Longer than P75 for all trials

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2015

Completed
3.1 years until next milestone

First Submitted

Initial submission to the registry

December 12, 2018

Completed
7 days until next milestone

First Posted

Study publicly available on registry

December 19, 2018

Completed
10 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 30, 2019

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2019

Completed
Last Updated

August 28, 2019

Status Verified

August 1, 2019

Enrollment Period

3.9 years

First QC Date

December 12, 2018

Last Update Submit

August 27, 2019

Conditions

Keywords

breast cancerbiomarkerneoadjuvant treatmentmicroRNAresponse

Outcome Measures

Primary Outcomes (1)

  • Objective Response

    Clinical disease response was evaluated for every two cycles of chemotherapy till surgery with RECIST criteria (RECIST 1.1). Resistant group was defined ad progression disease (PD) and/or stable disease (SD).Evaluation in breast lesions by MRI or mammography. The same imaging methods were used throughout treatment for a given patient. Blood samples for microRNA were collected before neoadjuvant chemotherapy, evaluation of clinical disease response and surgery. Clinical disease response was evaluated for every two cycles of chemotherapy till surgery with RECIST criteria (RECIST 1.1). Evaluation in breast lesions by MRI or mammography. The same imaging methods were used throughout treatment for a given patient.

    Every 2 cycles, up to surgery (each cycle is 21 days)

Study Arms (2)

Sensitive

Sensitive group was defined ad complete response (CR) and/or partial response (PR). Blood samples for microRNA were collected before neoadjuvant chemotherapy, evaluation of clinical disease response and surgery.

Genetic: microRNA

Resistant

Resistant group was defined ad progression disease (PD) and/or stable disease (SD). Blood samples for microRNA were collected before neoadjuvant chemotherapy, evaluation of clinical disease response and surgery.

Genetic: microRNA

Interventions

microRNAGENETIC

The level of microRNA in plasma will be detected by TaqMan in screening phase and by quantitative Real-time PCR (qRT-PCR) in validation phase.

ResistantSensitive

Eligibility Criteria

Age18 Years - 75 Years
Sexfemale(Gender-based eligibility)
Gender Eligibility DetailsFemale patients was eligible.
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Female, Breast Cancer, \>18 years,\<75 years, early stage breast cancer patients, with Stage II-III disease

You may qualify if:

  • Early breast cancer patients;
  • Stage II-III disease;
  • sign informed consent form;
  • receive neoadjuvant treatment;
  • Age between 18-75.

You may not qualify if:

  • Women during pregnancy;
  • Metastasis patients or stage IV breast cancer patients;
  • Male breast cancer patients;
  • Inflammatory breast cancer patients;
  • Patients with neoadjuvant endocrine treatment.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Peking University First Hospital

Beijing, Beijing Municipality, 100034, China

RECRUITING

Related Publications (4)

  • Li Q, Liu M, Ma F, Luo Y, Cai R, Wang L, Xu N, Xu B. Circulating miR-19a and miR-205 in serum may predict the sensitivity of luminal A subtype of breast cancer patients to neoadjuvant chemotherapy with epirubicin plus paclitaxel. PLoS One. 2014 Aug 19;9(8):e104870. doi: 10.1371/journal.pone.0104870. eCollection 2014.

    PMID: 25137071BACKGROUND
  • Wang H, Tan G, Dong L, Cheng L, Li K, Wang Z, Luo H. Circulating MiR-125b as a marker predicting chemoresistance in breast cancer. PLoS One. 2012;7(4):e34210. doi: 10.1371/journal.pone.0034210. Epub 2012 Apr 16.

    PMID: 22523546BACKGROUND
  • Zhao R, Wu J, Jia W, Gong C, Yu F, Ren Z, Chen K, He J, Su F. Plasma miR-221 as a predictive biomarker for chemoresistance in breast cancer patients who previously received neoadjuvant chemotherapy. Onkologie. 2011;34(12):675-80. doi: 10.1159/000334552. Epub 2011 Nov 23.

    PMID: 22156446BACKGROUND
  • Zhu W, Liu M, Fan Y, Ma F, Xu N, Xu B. Dynamics of circulating microRNAs as a novel indicator of clinical response to neoadjuvant chemotherapy in breast cancer. Cancer Med. 2018 Sep;7(9):4420-4433. doi: 10.1002/cam4.1723. Epub 2018 Aug 11.

    PMID: 30099860BACKGROUND

Biospecimen

Retention: SAMPLES WITHOUT DNA

For each patient, 4 mL of peripheral blood will be collected as per predesigned schedule. Within 4 hours of blood drawl, samples will be centrifuged at 1500g for 10min at 4℃ to separate the plasma supernatant. The plasma and the blood cell will be aliquoted and stored at -80℃ until analysis, respectively.

MeSH Terms

Conditions

Breast Neoplasms

Interventions

MicroRNAs

Condition Hierarchy (Ancestors)

Neoplasms by SiteNeoplasmsBreast DiseasesSkin DiseasesSkin and Connective Tissue Diseases

Intervention Hierarchy (Ancestors)

RNA, AntisenseAntisense Elements (Genetics)Nucleic Acids, Nucleotides, and NucleosidesRNANucleic AcidsRNA, Small UntranslatedRNA, Untranslated

Study Officials

  • Yimin Cui, Ph.D & M.D

    Peking University First Hospital

    STUDY DIRECTOR

Central Study Contacts

Qian Xiang, Ph.D

CONTACT

Zhuo Zhang, M.Sc

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Director of pharmacy, M.D & Ph.D

Study Record Dates

First Submitted

December 12, 2018

First Posted

December 19, 2018

Study Start

November 1, 2015

Primary Completion

September 30, 2019

Study Completion

December 31, 2019

Last Updated

August 28, 2019

Record last verified: 2019-08

Data Sharing

IPD Sharing
Will not share

We would not like to share individual participant data (IPD) but share the result after statistical analysis. If IPD was needed, please contact us by e-mail.

Locations