Estimation of Level of ERAP1 Expression in Lesional and Non Lesional Skin of Psoraitic Patients and Healthy Controls.

NCT03767114 | Completed

• 1 other identifier: EgyERAP
• Study Type: interventional
• Target: 55
• Locations: 0 countries
• Sites: N/A

Brief Summary

A 4mm Punch biopsy is obtained from lesional and non lesional skin of 25 psoraitic patients as well as of 30 controls. Skin biopsy from patients (psoriasis lesional and non lesional) \& control will be kept in lysis solution (which contains RNAase inhibitor) for the stability of the parameter to be assessed. ERAP-1 gene expression will Research Template 5 Final Version: 8/3/2 016 be detected by quantitative real time -PCR, which include RNA extraction, cDNA synthesis \& PCR.

Conditions

Psoriasis

Outcome Measures

Primary Outcomes (1)

• Estimation of lesional ERAP1 in psoriatic skin

  DPCRetection of level of expression of ERAP 1 in lesional skin of psoriasis patients by RT-

  Maximum 2 years

Secondary Outcomes (1)

• Estimation of non lesional ERAP1 in psoriatic skin

  Maximum 2 years

Study Arms (2)

cases of psoriasis

EXPERIMENTAL

group of 25 cases of psoriasis subjected to skin biopsy for detection of lesional and non lesional expression of ERAP1 by RT-PCR

Diagnostic Test: RT-PCR for ERAP1 detection

normal controls

EXPERIMENTAL

group of 30 age and sex matched healthy controls o subjected to skin biopsy from normal skin for detection of expression of ERAP1 by RT-PCR

Diagnostic Test: RT-PCR for ERAP1 detection

Interventions

RT-PCR for ERAP1 detection DIAGNOSTIC_TEST

Reverse transcriptase -Polymerase Chain Reaction for ERAP1 detection

cases of psoriasis; normal controls

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• patients with psoriasis vulgaris
• patients over 18 years of age
• both sexes

You may not qualify if:

• patients on treatment for psoriasis within the last two months
• assoiciated autoimmune diseases eg SLE
• pregnancy

Sponsors & Collaborators

• Kasr El Aini Hospital: lead

MeSH Terms

Conditions

Psoriasis

Condition Hierarchy (Ancestors)

Skin Diseases, Papulosquamous; Skin Diseases; Skin and Connective Tissue Diseases

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: DIAGNOSTIC
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Lecturer of dermatology

Study Record Dates

• First Submitted: December 5, 2018
• First Posted: December 6, 2018
• Study Start: January 1, 2017
• Primary Completion: June 1, 2017
• Study Completion: December 1, 2018
• Last Updated: December 6, 2018

Record last verified: 2018-12