Effects of Different Genetic Mutations on Prognosis in sMPLC Adenocarcinoma Patients

NCT03764371 | Unknown

• 1 other identifier: K2018108
• Study Type: observational
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

In 2007 and 2013, the American College of Chest Physicians (ACCP) guidelines applied the diagnostic criteria of sMPLC （synchronous multiple primary lung cancers）, and the diagnostic criteria of Martini and Melamed were extended and developed, Summarized as: (1) different histological types, different genetic characteristics, or different origin of carcinoma in situ; (2) the histological type is the same, the tumor is located in different lung or different lung lobes, the common lymphatic drainage site of lung cancer is not cancerous, and there is no extrapulmonary metastasis at the time of diagnosis. Postoperative staging of each tumor was carried out in sMPLC patients, if all of them were stage I lung adenocarcinoma, whether adjuvant therapy could fully refer to the treatment principle of stage I NSCLC was considered, whether the benefit of subsequent application of adjuvant chemotherapy was still unclear, and whether adjuvant therapy was needed or not has been determined. High-throughput sequencing, also known as "Next generation" sequencing (NGS), is characterized by sequencing of hundreds of thousands to millions of DNA molecules in parallel, and generally shorter reads.For multiple tumor lesions resected by sMPLC, only biopsy gene information from a single cancer focus may not be enough to identify all active driver gene mutations from the tumor. Therefore, NGS sequencing was proposed for all cancer lesions of sMPLC patients to reflect the full picture of gene mutation in such patients. The investigators initiated this prospective clinical study to detect lung cancer related genes in tumor tissues and patients with at least 2 tumors that were confirmed as invasive adenocarcinoma by pathology after sMPLC resection (residual non-resectable or non-qualitative pulmonary nodules). At the same time, application of NGS technology to test lung cancer related genes in patients' tumor tissues and blood, patients with lung cancer drive genes were followed up to explore whether different drive genes had an impact on patients' disease progression. In order to investigate the type of gene that causes disease recurrence in patients, tissue or blood test was performed again when disease recurrence occur.

Conditions

Gene Mutation

Outcome Measures

Primary Outcomes (1)

• DFS

  Disease-free survival

  Two years

Secondary Outcomes (1)

• Drive-gene about recurrence

  Five years

Study Arms (1)

sMPLC

Lung cancer related genes in tumor tissues and patients with at least 2 tumors that were confirmed as invasive adenocarcinoma by pathology after sMPLC resection (residual non-resectable or non-qualitative pulmonary nodules).

Diagnostic Test: KAPA Hyper Prep Kit + Agilent SureSelectQXT

Interventions

KAPA Hyper Prep Kit + Agilent SureSelectQXT DIAGNOSTIC_TEST

We initiated this prospective clinical study to detect lung cancer related genes in tumor tissues and patients with at least 2 tumors that were confirmed as invasive adenocarcinoma by pathology after sMPLC resection (residual non-resectable or non-qualitative pulmonary nodules). At the same time, application of KAPA Hyper Prep Kit + Agilent SureSelectQXT technology to test lung cancer related genes in patients' tumor tissues and blood, patients with lung cancer drive genes were followed up to explore whether different drive genes had an impact on patients' disease progression. In order to investigate the type of gene that causes disease recurrence in patients, tissue or blood test was performed again when disease recurrence occur.

Also known as: NGS technology

sMPLC

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

The investigators initiated this prospective clinical study to detect lung cancer related genes in tumor tissues and patients with at least 2 tumors that were confirmed as invasive adenocarcinoma by pathology after sMPLC resection (residual non-resectable or non-qualitative pulmonary nodules). At the same time, application of NGS technology to test lung cancer related genes in patients' tumor tissues and blood, patients with lung cancer drive genes were followed up to explore whether different drive genes had an impact on patients' disease progression. In order to investigate the type of gene that causes disease recurrence in patients, tissue or blood test was performed again when disease recurrence occur.

You may qualify if:

• Male or female patients: 18-75 years old;
• ECOG score: 0-1;
• At least two tumors in patients with invasive lung adenocarcinoma at stage I are pathologically confirmed after sMPLC surgery;
• Genetic test is performed on the pathological tissues of the tumor lesions excised, and at least one with driver gene;
• Predicted survival ≥1 year；
• No more than 3 months after sMPLC surgery (last operation);
• Good compliance, family members agree to cooperate to receive survival follow-up;
• Understand and voluntarily sign the informed consent.

You may not qualify if:

• Previous or co-existing malignant tumors (patients with resected basal cell carcinoma or other carcinoma in situ are not included);
• Systemic anti-tumor therapy, including chemotherapy, radiotherapy or targeted therapy (including but not limited to monoclonal antibodies, small-molecule tyrosine kinase inhibitors, etc.) was used before enrollment.
• Participated in clinical trials of other drugs within 4 weeks
• All the mutations are insignificant to lung cancer;
• The investigator is not sure that the subject will be able to complete the study ( management reasons or others).

Sponsors & Collaborators

• The First Hospital of Jilin University: lead

Study Sites (1)

The first hospital of Jilin University

Changchun, Jilin, China

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

We use application of NGS technology to test lung cancer related genes in patients' tumor tissues and blood, patients with lung cancer drive genes were followed up to explore whether different drive genes had an impact on patients' disease progression.

Central Study Contacts

Kewei F Ma

CONTACT

008613756060506; makw@jlu.edu.cn

Ye F Guo

CONTACT

008615526642279; year0904@sina.com

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 28, 2018
• First Posted: December 5, 2018
• Study Start: April 22, 2019
• Primary Completion: August 30, 2022
• Study Completion: March 30, 2024
• Last Updated: October 14, 2021

Record last verified: 2021-08

Locations

CN (1)