NCT03663140

Brief Summary

Regulatory effects of some neuropeptides substance-P (SP), neurokinin-A (NKA), calcitonin gene-linked peptide (CGRP) and neuropeptide-Y (NPY) )on inflammatory responses in periodontal disease has been described; however, the impact of neuropeptide levels are not clearly defined in healthy and diseased peri-implant tissues.ın order to evaluate the situation, thirty-nine implants that have been loaded over the past 12 months and their symmetrically matching teeth were evaluated using a split-mouth study design. Clinical periodontal examinations included the Silness-Löe plaque index, Löe-Silness gingival index, bleeding on probing, probing pocket depth and clinical attachment level parameters were determined. Gingival crevicular fluid (GCF)/Periimplant sulcular fluid (PISF) samples were collected, and the levels of the neuropeptides were determined by enzyme-linked immunosorbent assay. Correlations between GCF/PISF neuropeptide levels and the clinical examination parameters were evaluated in the peri-implant/periodontal soft tissues.

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
39

participants targeted

Target at P25-P50 for all trials

Timeline
Completed

Started Jan 2011

Typical duration for all trials

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 1, 2011

Completed
2.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 15, 2013

Completed
4 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 30, 2013

Completed
4.7 years until next milestone

First Submitted

Initial submission to the registry

September 6, 2018

Completed
4 days until next milestone

First Posted

Study publicly available on registry

September 10, 2018

Completed
Last Updated

September 13, 2018

Status Verified

September 1, 2018

Enrollment Period

2.7 years

First QC Date

September 6, 2018

Last Update Submit

September 11, 2018

Conditions

Outcome Measures

Primary Outcomes (3)

  • Gingival crevicular fluid/peri-implant sulcular fluid Level of Substance-P

    Substance-P (SP) is a neuropeptide of the tachykinin family and marker of neurogenic inflammation.

    8-10 am on the day following periodontal status assessment.

  • Gingival crevicular fluid/peri-implant sulcular fluid Level of neurokinin-A

    Neurokinin-A is a neuropeptide of the tachykinin family and marker of neurogenic inflammation.

    8-10 am on the day following periodontal status assessment.

  • Gingival crevicular fluid/peri-implant sulcular fluid Level of Calcitonin gene-related peptide

    Calcitonin gene-related peptide (CGRP), another neuropeptide derived from peptidergic nerves and a marker of neurogenic inflammation.

    8-10 am on the day following periodontal status assessment.

Study Arms (6)

Group 1-Health periodontal

This group created by individuals with healthy periodontal tissues. Periodontal status/peri-implant was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions.

Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection

Group 2- Healthy peri-implant

This group created by individuals with healthy peri-implant tissues. Periodontal status/peri-implant was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions.

Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection

group 3- Gingivitis

This group created by individuals with gingivitis. Periodontal/peri-implant status was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions.

Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection

Group 4-Peri-implant Mucositis

This group created by individuals with peri-implant mucositis. Periodontal/peri-implant status was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions.

Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection

Group 5-Periodontitis

This group created by individuals with periodontitis. Periodontal/peri-implant status was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions.

Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection

Group 6-Periimplantitis

This group created by individuals with peri-implantitis. Periodontal/peri-implant status was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions.

Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection

Interventions

GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.

Group 1-Health periodontalGroup 2- Healthy peri-implantGroup 4-Peri-implant MucositisGroup 5-PeriodontitisGroup 6-Periimplantitisgroup 3- Gingivitis

Eligibility Criteria

Age18 Years - 65 Years
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Thirty-nine male, systemically healthy adult individuals (35-55 years old) and 39 dental implants were recruited for the present study. The same implant system was utilized for the treatments and the clinical treatment procedures were completed by the same two clinicians at the Ondokuz Mayis University Dental Faculty between 2011 and 2013.

You may qualify if:

  • Compliance with systematic maintenance care;
  • Adult, non-smoker, systemically healthy
  • Not using any medicament for the last 6 months ;
  • Partially dentated patient treated with implant-supported metal ceramic full crowns and/or fixed partial dentures
  • As the study was split-mouth designed, in each individual at least one implant and one matching tooth in the same mouth symmetrically representing the same tooth group (premolars/molars);
  • A single type of metal ceramic implant restoration that is functional for at least 1 year; and a matching tooth with no dental or endodontic restorations.

You may not qualify if:

  • Medical history of cancer, rheumatoid arthritis, diabetes mellitus, or cardiovascular disease and any other systemic disease affecting lipid metabolism (i.e. impaired glucose tolerance, metabolic syndrome);
  • Compromised immune system;
  • Pregnancy, menopause, or lactation;
  • Ongoing drug therapy that might affect the clinical characteristics of periodontitis and lipid metabolism;
  • Dental treatment during the 6 months prior to data collection.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Biospecimen

Retention: SAMPLES WITHOUT DNA

gingival crevicular fluid

MeSH Terms

Conditions

Neurogenic InflammationPeriodontal Diseases

Condition Hierarchy (Ancestors)

Neurologic ManifestationsNervous System DiseasesInflammationPathologic ProcessesPathological Conditions, Signs and SymptomsMouth DiseasesStomatognathic Diseases

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
CROSS SECTIONAL
Target Duration
2 Days
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
assoc. prof.dr

Study Record Dates

First Submitted

September 6, 2018

First Posted

September 10, 2018

Study Start

January 1, 2011

Primary Completion

September 15, 2013

Study Completion

December 30, 2013

Last Updated

September 13, 2018

Record last verified: 2018-09