Effect of Chorioamnionitis on Platelet Activation and Placental Vessel Among Preterm Infants by Wnt-Flt1 Signal Pathway

NCT03636698 | Completed

• 1 other identifier: Guangdong W&C Hospital
• Study Type: observational
• Target: 64
• Locations: 0 countries
• Sites: N/A

Brief Summary

Objective:The purpose of this study was to explore the effect and mechanism of maternal chorioamnionitis on placental microvasculature and platelet activation among preterm infants by activating Wnt-Flt1 signal pathway . Methods:With clinical randomized controlled trial (RCT), the cases were matched with 1:1 according to gestational age and divided into 2 groups according to the placental pathology result: chorioamnionitis group and control group. (1) To observe the platelet parameter, birth weight, thrombrocytopenia and hemorrhage complication, such as intracranial hemorrhage, retinal hemorrhage, pulmonary hemorrhage and gastrointestinal hemorrhage. (2) To observe the miscrovascular density (MVD) in placenta, platelet activating factor (CD62p,CD63) and thrombopotetin (TPO) in preterrn infants.The placental MVD was assessed by immunohistochemical method. The platelet activating factors were detected by flow cytometry. TPO was detected by ELISA. (3) To observe Wnt5a, Flt1 and VEGF in placenta and fetal circulation.The measurement data were analyzed by pair t test and conditional logistic regression. Pearson correlation analysis was used for relationship.

Conditions

Chorioamnionitis; Placenta Diseases; Thrombocytopenia; Preterm Infant

Outcome Measures

Primary Outcomes (1)

• Placental Microvascular Density(%)

  Placental Microvascular Density (by placental histopathology)

  24 hours

Secondary Outcomes (12)

• Incidence Hemorrhage Disease (%)

  through study completion, an average of half year

• Wnt5a in Placenta(IOD)

  24 hours

• mean platelet volume(MPV)

  24 hours

• platelet distribution width(PDW)

  24 hours

• Platelet counts (PLT)

  24 hours

Study Arms (2)

Chorioamnionitis Group

preterm infants were born to mothers with chorioamnionitis

Other: chorioamnionitis

Control Group

preterm infants were born to mothers without chorioamnionitis

Other: chorioamnionitis

Interventions

chorioamnionitis OTHER

It is a observation research. We didn't intervene anything. The chorioamnionitis groups included the infants whose mothers were diagnosed chorioamnionitis by placental histopathology.

Chorioamnionitis Group; Control Group

Eligibility Criteria

• Age: 1 Hour+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Chorioamnionitis contributes to the high morbidity and mortality in preterm infants, thrombocytopenia is one of the acute morbidity

You may qualify if:

• gestational age\<37weeks
• admitted to the NICU at Guangdong Women and Children Hospital, with a date of birth from June 2016 to December 2016
• whose mothers were diagnosed chorioamnionitis by placental histopathology

You may not qualify if:

• born to mother with no any other maternal complications
• no congenital abnormalities and neonatal asphyxia;
• died or discharged within 72 hours.

Sponsors & Collaborators

• Guangdong Women and Children Hospital: lead

Biospecimen

Retention: SAMPLES WITHOUT DNA

the placentas and cord blood after delivery

MeSH Terms

Conditions

Chorioamnionitis; Placenta Diseases; Thrombocytopenia; Premature Birth

Condition Hierarchy (Ancestors)

Fetal Diseases; Pregnancy Complications; Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases; Fetal Membranes, Premature Rupture; Obstetric Labor Complications; Congenital, Hereditary, and Neonatal Diseases and Abnormalities; Blood Platelet Disorders; Hematologic Diseases; Hemic and Lymphatic Diseases; Cytopenia; Obstetric Labor, Premature

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Chief of Neontology Dept

Study Record Dates

• First Submitted: August 1, 2018
• First Posted: August 17, 2018
• Study Start: June 1, 2016
• Primary Completion: December 31, 2016
• Study Completion: December 31, 2016
• Last Updated: August 17, 2018

Record last verified: 2016-06