Israeli Multi Ethnic Centenarian Project

NCT03541460 | Completed

• 1 other identifier: 0312-14-RMB
• Study Type: observational
• Target: 124
• Locations: 1 country
• Sites: 1

Brief Summary

This study will systematically assess epigenome methylation changes in participants of the Israel multi-ethnic centenarian study cohort, which includes citizens of Israel aged 95 years and older, compared to their offspring and younger controls.

Conditions

LONGEVITY 1

Keywords

centenarians; epigenome; longevity; healthy aging; epigenetic

Outcome Measures

Primary Outcomes (1)

• Frequency of alleles in genes across the genome

  Variations of frequency of alleles in study groups using dedicated software for genomic analysis.

  6 months

Study Arms (2)

Older Cohort

Demographic, health and functional data will be collected from subjects 95 years and older by means of a structured interview. Blood will be collected for laboratory and genetic testing.

Younger Controls

Demographic, health and functional data will be collected from the children of the older subjects and other aged-matched controls by means of a structured interview. Blood will be collected for laboratory and genetic testing.

Eligibility Criteria

• Age: 95 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Subjects aged 95 years and older will form the study cohort. Control group will comprise children of the older subjects and age-matched controls.

You may qualify if:

• Aged 95 years and older.
• Provide verbal consent for participation in the study.
• Cognitively able to provide consent and answer a structured questionnaire.

You may not qualify if:

• Not able to provide consent for participation in the study.
• Impaired consciousness.
• Unable to communicate.

Sponsors & Collaborators

• Rambam Health Care Campus: lead
• Israel Science Foundation: collaborator

Study Sites (1)

Rambam Health Care Campus

Haifa, North, 3109601, Israel

Location

Related Publications (3)

• Brooks-Wilson AR. Genetics of healthy aging and longevity. Hum Genet. 2013 Dec;132(12):1323-38. doi: 10.1007/s00439-013-1342-z. Epub 2013 Aug 8.

  PMID: 23925498 BACKGROUND

• Evert J, Lawler E, Bogan H, Perls T. Morbidity profiles of centenarians: survivors, delayers, and escapers. J Gerontol A Biol Sci Med Sci. 2003 Mar;58(3):232-7. doi: 10.1093/gerona/58.3.m232.

  PMID: 12634289 BACKGROUND

• Wojdacz TK, Hansen LL. Techniques used in studies of age-related DNA methylation changes. Ann N Y Acad Sci. 2006 May;1067:479-87. doi: 10.1196/annals.1354.069.

  PMID: 16804030 BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

Three 10 ml blood samples will be drawn into vacutainer tubes (Yellow top with anticoagulant; speckled top without anticoagulant). Blood samples will be analyzed for the levels of hemoglobin, hematocrit, white blood cell counts, and levels of glucose, insulin, albumin, inflammatory markers, and plasma lipids. CD34+Lin- cells will be isolated from the peripheral blood specimen using immuno-magnetic separation technique, followed by cell sorter. Mononuclear cells are separated by Ficoll-Paque density gradient, and CD34+ cells obtained by positive immuno-magnetic bead selection, using Macs columns (Miltenyi Biotech). The purified cells are then subjected to a cell sorter following immunostaining. The isolated cells will be cryopreserved in 10% DMSO by controlled rate freezing.

Study Officials

• Tzvi Dwolatzky, MD

  Rambam Health Care Campus

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Director of Geriatric Medicine

Study Record Dates

• First Submitted: January 10, 2017
• First Posted: May 30, 2018
• Study Start: January 1, 2016
• Primary Completion: December 31, 2022
• Study Completion: December 31, 2022
• Last Updated: April 4, 2023

Record last verified: 2023-04

Data Sharing

• IPD Sharing: Will not share

Locations

IL (1)