NCT03333681

Brief Summary

  1. 1.Objectives: Evaluation of mesenchymal stem cell therapy effects on cellular and humoral immune responses in refractory rheumatoid arthritis (RA) patients.
  2. 2.Design: This study has been performed as a phase 1 clinical trial.
  3. 3.Setting and conduct: Autologous bone marrow derived mesenchymal stem cells were obtained by bone marrow aspiration and cultured in Minimum Essential Medium-Alpha (MEM-alpha) for 4 weeks in standard clean room and then transfused to RA patients intravenously.
  4. 4.Participant's major eligibility criteria is as follows: Ten refractory rheumatoid arthritis patients who show resistance to non biological Disease-modifying antirheumatic drugs (DMARDs) after 6 to 12 months have been enrolled in this study.
  5. 5.Intervention: A single dose of intravenous autologous bone marrow derived mesenchymal stem cells have been administered into patients with resistance to non-biological DMARDs.
  6. 6.Main outcome measures (variables): Finding of mesenchymal stem cell therapy effects on the cellular and humoral immune responses and evaluation of the effect of chemokines in homing of immune cells following the intervention.

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
15

participants targeted

Target at below P25 for phase_1 rheumatoid-arthritis

Timeline
Completed

Started Jun 2016

Typical duration for phase_1 rheumatoid-arthritis

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 20, 2016

Completed
1.2 years until next milestone

First Submitted

Initial submission to the registry

September 14, 2017

Completed
2 months until next milestone

First Posted

Study publicly available on registry

November 7, 2017

Completed
9 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

August 15, 2018

Completed
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

September 15, 2018

Completed
Last Updated

March 13, 2019

Status Verified

March 1, 2019

Enrollment Period

2.2 years

First QC Date

September 14, 2017

Last Update Submit

March 12, 2019

Conditions

Keywords

Rheumatoid arthritisStem CellsCellular immunityHumoral immunitychemokineshoming of immune cells

Outcome Measures

Primary Outcomes (1)

  • Effect of mesenchymal stem cells therapy on the percentage of regulatory T cells

    Percentage change in regulatory T cells from baseline which is analysed by fluorescence-activated cell sorting (FACS)

    At 0 and 6 months follow up

Study Arms (1)

Refractory rheumatoid arthritis patients

EXPERIMENTAL

Autologous mesenchymal stem cells

Biological: Autologous mesenchymal stem cells

Interventions

A single intravenous administration of autologous bone marrow derived mesenchymal stem cells(1000000 to 2000000 cells/Kg)

Refractory rheumatoid arthritis patients

Eligibility Criteria

Age35 Years - 60 Years
Sexfemale(Gender-based eligibility)
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • Resistant RA patients to non-biological DMARDs;
  • Treated RA patients by non-biological drugs;
  • Treated RA patients by Prednisolone, Hydroxychloroquine, Sulfasalazine and Methotrexate;
  • Patients age between 35-60 years;
  • Refractory RA patients with no other rheumatologic disorders and inflammatory diseases.

You may not qualify if:

  • Non-resistant RA patients to non-biological DMARDs.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Publications (4)

  • Alvaro-Gracia JM, Jover JA, Garcia-Vicuna R, Carreno L, Alonso A, Marsal S, Blanco F, Martinez-Taboada VM, Taylor P, Martin-Martin C, DelaRosa O, Tagarro I, Diaz-Gonzalez F. Intravenous administration of expanded allogeneic adipose-derived mesenchymal stem cells in refractory rheumatoid arthritis (Cx611): results of a multicentre, dose escalation, randomised, single-blind, placebo-controlled phase Ib/IIa clinical trial. Ann Rheum Dis. 2017 Jan;76(1):196-202. doi: 10.1136/annrheumdis-2015-208918. Epub 2016 Jun 7.

    PMID: 27269294BACKGROUND
  • Liang J, Li X, Zhang H, Wang D, Feng X, Wang H, Hua B, Liu B, Sun L. Allogeneic mesenchymal stem cells transplantation in patients with refractory RA. Clin Rheumatol. 2012 Jan;31(1):157-61. doi: 10.1007/s10067-011-1816-0. Epub 2011 Aug 12.

    PMID: 21837432BACKGROUND
  • Wang L, Wang L, Cong X, Liu G, Zhou J, Bai B, Li Y, Bai W, Li M, Ji H, Zhu D, Wu M, Liu Y. Human umbilical cord mesenchymal stem cell therapy for patients with active rheumatoid arthritis: safety and efficacy. Stem Cells Dev. 2013 Dec 15;22(24):3192-202. doi: 10.1089/scd.2013.0023. Epub 2013 Oct 4.

    PMID: 23941289BACKGROUND
  • Rahimi Khorashad M, Ghoryani M, Gowhari Shabgah A, Shariati-Sarabi Z, Tavakkol Afshari J, Mohammadi M. The Effects of Mesenchymal Stem Cells on the Gene Expression of TGF-beta and IFN-gamma in Patients with Rheumatoid Arthritis. Iran J Allergy Asthma Immunol. 2023 Apr 30;22(2):183-189. doi: 10.18502/ijaai.v22i2.12679.

MeSH Terms

Conditions

Arthritis, Rheumatoid

Condition Hierarchy (Ancestors)

ArthritisJoint DiseasesMusculoskeletal DiseasesRheumatic DiseasesConnective Tissue DiseasesSkin and Connective Tissue DiseasesAutoimmune DiseasesImmune System Diseases

Study Officials

  • Mojgan Mohammadi, Ph.D

    Mashhad University of Medical Sciences

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate professor

Study Record Dates

First Submitted

September 14, 2017

First Posted

November 7, 2017

Study Start

June 20, 2016

Primary Completion

August 15, 2018

Study Completion

September 15, 2018

Last Updated

March 13, 2019

Record last verified: 2019-03

Data Sharing

IPD Sharing
Will not share