NCT03326505

Brief Summary

This study aims at expanding Umbilical Cord derived Mesenchymal Stem Cells (MSCs) to a clinical scale according to Good Laboratory Practice and study its efficacy when compared to a Supervised Physical Therapy Program alone.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
60

participants targeted

Target at P75+ for phase_1 multiple-sclerosis

Timeline
Completed

Started Sep 2017

Geographic Reach
1 country

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 9, 2017

Completed
5 months until next milestone

Study Start

First participant enrolled

September 25, 2017

Completed
1 month until next milestone

First Posted

Study publicly available on registry

October 31, 2017

Completed
2.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 25, 2020

Completed
26 days until next milestone

Study Completion

Last participant's last visit for all outcomes

February 20, 2020

Completed
Last Updated

March 4, 2020

Status Verified

February 1, 2020

Enrollment Period

2.3 years

First QC Date

May 9, 2017

Last Update Submit

February 28, 2020

Conditions

Keywords

Umbilical Cord Mesenchymal Stem CellsMultiple SclerosisNeuroimmunologyAutoimmune diseasePhysical therapy

Outcome Measures

Primary Outcomes (1)

  • Isolation and Expansion of Mesenchymal Stem Cells

    To isolate stem cells from UC: first, the cord will be cut into small pieces around (4-5cm) and washed with phosphate buffered saline, then the umbilical vein and the two arteries will be removed.These pieces will be placed in culture plate and cultured in UC-MSCs medium consist of; alpha modification of Minimum Essential Medium (α-MEM) supplemented with 10% fetal bovine serum, 1% glutamine, 1% penicillin/streptomycin and 1 μg/mL amphotericin B.After incubation period the undigested tissue will be discarded and nucleated cells will be pelleted by centrifugation at 450xg for 10 minutes. Cell pellet will be re-suspended in UC-MSCs culture medium, cultured in tissue culture flasks and placed in CO2 incubator at 37°C, 5% CO2. Cells will be expanded in multilayer cell factory tissue culture flasks to reach an estimated 108 UC-MSCs that will be frozen in bags of 106 cells for subsequent thawing and administration.

    3-6 months

Secondary Outcomes (4)

  • Safety and Efficacy Assessment Pre and Post Treatment

    3-12 months

  • Motor Functions Assessments

    3-12 months

  • Measuring Non-motor outcomes to assess treatment efficacy.

    3-6 months

  • Biological Assessments

    3-12 months

Study Arms (3)

Injection of Umbilical cord derived UC- MSCs

ACTIVE COMPARATOR

Allogenic Umbilical Cord derived stem cells injected intrathecally to enrolled MS patients

Biological: Umbilical cord derived Mesenchymal Stem Cells

injection of UC- MSCs and SPT

ACTIVE COMPARATOR

Allogenic Umbilical Cord derived stem cells injected intrathecally to enrolled MS patients along with a supervised physical therapy program

Biological: Umbilical cord derived Mesenchymal Stem CellsOther: Supervised physical therapy

Supervised Physical Therapy (SPT)

ACTIVE COMPARATOR

Supervised physical therapy program without stem cells

Other: Supervised physical therapy

Interventions

Allogenic umbilical cord derived stem cells which are to be injected intrathecally as a treatment option for consenting MS patients

Also known as: UC-MSCs
Injection of Umbilical cord derived UC- MSCsinjection of UC- MSCs and SPT

A combined physical therapy program of Balance, strengthening and endurance exercises

Also known as: SPT
Supervised Physical Therapy (SPT)injection of UC- MSCs and SPT

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • A clinical diagnosis of definite MS according to the revised McDonald Criteria.
  • Expanded Disability Status Scale (EDSS) ≤ 7
  • Failure of standard medical therapy
  • Disease duration of at least three years prior to enrollment.

You may not qualify if:

  • Pregnant and lactating women
  • Previous treatment with immunosuppressive agents in the last 12 months prior to enrollment
  • Recent MS relapse in the month prior to enrollment
  • Treatment with oral or parenteral steroids for any cause in the month prior to enrollment
  • Significant systemic medical disorders including cardiac, renal, hepatic, hematologic, immunologic or endocrine disorders
  • Previous treatment with interferons or glatiramer acetate in the 3 months prior to enrollment
  • Any contra-indication for magnetic resonance imaging (MRI) or gadolinium contrast.
  • Positive serology for HIV, Hepatitis B or Hepatitis C
  • Any history of malignancy or exposure to radiation at any time prior to enrollment
  • Any contra-indication to lumbar puncture
  • Severe cognitive impairment that would interfere with the patient's ability to understand and sign the informed consent.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Cell Therapy Center, University of Jordan

Amman, 11942, Jordan

Location

Cell Therapy Center

Amman, 11942, Jordan

Location

Related Publications (1)

  • Alghwiri AA, Jamali F, Aldughmi M, Khalil H, Al-Sharman A, Alhattab D, Al-Radaideh A, Awidi A. The effect of stem cell therapy and comprehensive physical therapy in motor and non-motor symptoms in patients with multiple sclerosis: A comparative study. Medicine (Baltimore). 2020 Aug 21;99(34):e21646. doi: 10.1097/MD.0000000000021646.

MeSH Terms

Conditions

Multiple SclerosisAutoimmune Diseases

Condition Hierarchy (Ancestors)

Demyelinating Autoimmune Diseases, CNSAutoimmune Diseases of the Nervous SystemNervous System DiseasesDemyelinating DiseasesImmune System Diseases

Study Officials

  • Abdallah Awidi, MD

    Cell Therapy Center

    STUDY DIRECTOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
INVESTIGATOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Head of Neuroscience Research

Study Record Dates

First Submitted

May 9, 2017

First Posted

October 31, 2017

Study Start

September 25, 2017

Primary Completion

January 25, 2020

Study Completion

February 20, 2020

Last Updated

March 4, 2020

Record last verified: 2020-02

Data Sharing

IPD Sharing
Will not share

Locations