Apoptosis in Polymorphonuclear Cells and Inflammatory Cytokines of Trauma Patients

NCT03319017 | Unknown

• 1 other identifier: KUGH17229 (trauma)
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

This study tries to find how hypoxia and hyperoxia, among types of the respiratory failure induced cell damage that can arise in the course of traumatic damage and treatment, influences apoptosis of PMN cells and IL-6 and tumor necrosis factor(TNF)-α as inflammatory cytokines.

Conditions

Trauma

Keywords

Hypoxia; Hyperinflammatory; Hyperoxia; Apoptosis; Oxygenation; Polymorphonuclear Cells; Tumor necrosis factor(TNF)-α; Cytokine

Outcome Measures

Primary Outcomes (1)

• Measure interleukin(IL)-6

  * Measure in the separated plasma * Analyze correlation with oxygen saturation

  At the occurrence of the event during visiting an emergency room, up to 3 hours

Secondary Outcomes (2)

• Apoptosis of PMN check

  At the occurrence of the event during visiting an emergency room, up to 3 hours

• tumor necrosis factor(TNF)-α

  At the occurrence of the event during visiting an emergency room, up to 3 hours

Study Arms (1)

Multiple-trauma patients

The patients who are diagnosed with multiple-trauma and have blood test in an emergency room. The patients with multiple-trauma are defined as the patients who have trauma in more than two regions.

Procedure: Multiple-trauma patients

Interventions

Multiple-trauma patients PROCEDURE

The blood samples of the patients who agreed on this research are obtained in order to check their inflammatory response factor, and then their medical records are checked to find their initial ABGA result, lactate, C-reactive protein(CRP) known as initial inflammatory response, and procalcitonin. A patient's whole blood is collected in the tube with ethylene diaminetetracetic acid (EDTA) treatment. A modified method of Boyum's technique is applied so as to separate polymorphonuclear neutrophils. The separated polymorphonuclear neutrophils is collected so as to check apoptosis. And then, in the separated plasma, interleukin(IL)-6 and tumor necrosis factor(TNF)-α are measured, and its correlation with oxygen saturation will be analyzed.

Multiple-trauma patients

Eligibility Criteria

• Age: 19 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

The subjects of this study are patients with multiple severe trauma who have visited an emergency room in one year. this study is comparative observation research and have no intervention with treatment.

You may qualify if:

• Adults aged more than 19
• The patients who are diagnosed with multiple-trauma and have blood test in an emergency room. The patients with multiple-trauma are defined as the patients who have trauma in more than two regions.

You may not qualify if:

• The case where the check result of blood collection and oxygen saturation is able to influence treatment

Sponsors & Collaborators

• Korea University Guro Hospital: lead
• Korea University: collaborator

Study Sites (1)

Korea University Guro Hospital

Seoul, Guro-gu, 08308, South Korea

RECRUITING

Related Publications (14)

• Moon S, Lee SH, Ryoo HW, Kim JK, Ahn JY, Kim SJ, Jeon JC, Lee KW, Sung AJ, Kim YJ, Lee DR, Do BS, Park SR, Lee JS. Preventable trauma death rate in Daegu, South Korea. Clin Exp Emerg Med. 2015 Dec 28;2(4):236-243. doi: 10.15441/ceem.15.085. eCollection 2015 Dec.

  PMID: 27752603 BACKGROUND

• Deitch EA. Multiple organ failure. Pathophysiology and potential future therapy. Ann Surg. 1992 Aug;216(2):117-34. doi: 10.1097/00000658-199208000-00002.

  PMID: 1503516 BACKGROUND

• Gillani S, Cao J, Suzuki T, Hak DJ. The effect of ischemia reperfusion injury on skeletal muscle. Injury. 2012 Jun;43(6):670-5. doi: 10.1016/j.injury.2011.03.008. Epub 2011 Apr 9.

  PMID: 21481870 BACKGROUND

• Roumen RM, Redl H, Schlag G, Zilow G, Sandtner W, Koller W, Hendriks T, Goris RJ. Inflammatory mediators in relation to the development of multiple organ failure in patients after severe blunt trauma. Crit Care Med. 1995 Mar;23(3):474-80. doi: 10.1097/00003246-199503000-00010.

  PMID: 7874897 BACKGROUND

• Giannoudis PV, Hildebrand F, Pape HC. Inflammatory serum markers in patients with multiple trauma. Can they predict outcome? J Bone Joint Surg Br. 2004 Apr;86(3):313-23. doi: 10.1302/0301-620x.86b3.15035. No abstract available.

  PMID: 15125116 BACKGROUND

• Brach MA, deVos S, Gruss HJ, Herrmann F. Prolongation of survival of human polymorphonuclear neutrophils by granulocyte-macrophage colony-stimulating factor is caused by inhibition of programmed cell death. Blood. 1992 Dec 1;80(11):2920-4.

  PMID: 1280481 BACKGROUND

• Ciesla DJ, Moore EE, Zallen G, Biffl WL, Silliman CC. Hypertonic saline attenuation of polymorphonuclear neutrophil cytotoxicity: timing is everything. J Trauma. 2000 Mar;48(3):388-95. doi: 10.1097/00005373-200003000-00004.

  PMID: 10744274 BACKGROUND

• Elbim C, Estaquier J. Cytokines modulate neutrophil death. Eur Cytokine Netw. 2010 Mar;21(1):1-6. doi: 10.1684/ecn.2009.0183.

  PMID: 20146984 BACKGROUND

• Greenberg S, Grinstein S. Phagocytosis and innate immunity. Curr Opin Immunol. 2002 Feb;14(1):136-45. doi: 10.1016/s0952-7915(01)00309-0.

  PMID: 11790544 BACKGROUND

• Hsieh SC, Huang MH, Tsai CY, Tsai YY, Tsai ST, Sun KH, Yu HS, Han SH, Yu CL. The expression of genes modulating programmed cell death in normal human polymorphonuclear neutrophils. Biochem Biophys Res Commun. 1997 Apr 28;233(3):700-6. doi: 10.1006/bbrc.1997.6529.

  PMID: 9168918 BACKGROUND

• Kim JY, Hong YS, Choi SH, Yoon YH, Moon SW, Lee SW. Effect of hypertonic saline on apoptosis of polymorphonuclear cells. J Surg Res. 2012 Nov;178(1):401-8. doi: 10.1016/j.jss.2012.01.055. Epub 2012 Mar 23.

  PMID: 22475352 BACKGROUND

• Koller M, Clasbrummel B, Kollig E, Hahn MP, Muhr G. Major injury induces increased production of interleukin-10 in human granulocyte fractions. Langenbecks Arch Surg. 1998 Dec;383(6):460-5. doi: 10.1007/s004230050161.

  PMID: 9921948 BACKGROUND

• Menger MD, Vollmar B. Surgical trauma: hyperinflammation versus immunosuppression? Langenbecks Arch Surg. 2004 Nov;389(6):475-84. doi: 10.1007/s00423-004-0472-0. Epub 2004 May 28.

  PMID: 15173946 BACKGROUND

• Martin C, Boisson C, Haccoun M, Thomachot L, Mege JL. Patterns of cytokine evolution (tumor necrosis factor-alpha and interleukin-6) after septic shock, hemorrhagic shock, and severe trauma. Crit Care Med. 1997 Nov;25(11):1813-9. doi: 10.1097/00003246-199711000-00018.

  PMID: 9366763 BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

Whole blood collection

MeSH Terms

Conditions

Wounds and Injuries; Hypoxia; Hyperoxia

Condition Hierarchy (Ancestors)

Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Study Officials

• Jung-Youn Kim, MD, Ph.D.

  Assistant Professor

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Jung-Youn Kim, MD, Ph.D.

CONTACT

82-2-2626-3285; yellowwizard@hanmail.net

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: October 17, 2017
• First Posted: October 24, 2017
• Study Start: December 1, 2017
• Primary Completion: November 30, 2018
• Study Completion: November 30, 2018
• Last Updated: January 4, 2018

Record last verified: 2017-12

Locations

KR (1)