NCT03266471

Brief Summary

Inflammatory bowel disease (IBD), consisting of two major forms, Crohn's disease (CD) and ulcerative colitis (UC), affects more than 1.6 million people in the United States alone. Though the precise mechanisms underlying the inflammation and immune responses in IBD are still being investigated, various inflammatory mediators, including pro-inflammatory cytokines, and genes have been implicated in the disease process. At present, there are no reliable mechanisms to predict response to individual IBD medications. The investigators seek to evaluate whether inflammatory cytokines change longitudinally during treatment with anti-cytokine agents in both treatment primary responders and primary non-responders. In addition, the investigators will be evaluating the pharmacogenetics of treatment response to these agents.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
96

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Oct 2017

Longer than P75 for all trials

Geographic Reach
1 country

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

August 28, 2017

Completed
2 days until next milestone

First Posted

Study publicly available on registry

August 30, 2017

Completed
1 month until next milestone

Study Start

First participant enrolled

October 13, 2017

Completed
4.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 3, 2022

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

May 3, 2022

Completed
Last Updated

April 14, 2023

Status Verified

May 1, 2022

Enrollment Period

4.6 years

First QC Date

August 28, 2017

Last Update Submit

April 13, 2023

Conditions

Keywords

CytokinesPharmacogenetics

Outcome Measures

Primary Outcomes (1)

  • Compare longitudinal cytokine measurements in treatment responders versus nonresponders.

    The investigators will classify Crohn's disease subjects as primary responders or primary non-responders to anti-TNF-α, ustekinumab, or vedolizumab therapy based on clinical, endoscopic, and/or histologic data. The investigators will compare serum and tissue cytokines in CD subjects at before and after treatment initiation.

    52 weeks

Secondary Outcomes (2)

  • Compare serum and tissue pro-inflammatory cytokines in CD patients and healthy subjects.

    26 weeks

  • To assess whether gene variants are associated with treatment response.

    52 weeks

Study Arms (2)

Crohn's disease Patients

Patients that are seen in the Inflammatory Bowel Disease clinic with CD confirmed by endoscopy or radiology assessment who are initiating either an anti-tumor necrosis factor (TNF)-α agent (infliximab, adalimumab, certolizumab, or infliximab biosimilar), ustekinumab, or vedolizumab as part of their routine clinical care will be asked to provide blood samples, stool samples, and intestinal biopsies from standard of care colonoscopy at baseline and post therapy of at least 6 weeks duration but not more than 52 weeks.

Diagnostic Test: Baseline blood sampleDiagnostic Test: Baseline intestinal biopsiesOther: Crohn's disease activity index (CDAI)Diagnostic Test: Post treatment blood sampleDiagnostic Test: Post treatment intestinal biopsiesDiagnostic Test: Baseline stool sampleDiagnostic Test: Post treatment stool sample

Controls

Healthy adults without IBD undergoing colonoscopy for colorectal cancer screening or other non-IBD related indication will be asked to provide blood samples, stool samples and intestinal biopsies from standard of care colonoscopy.

Diagnostic Test: Baseline blood sampleDiagnostic Test: Baseline intestinal biopsiesDiagnostic Test: Baseline stool sample

Interventions

Baseline blood sampleDIAGNOSTIC_TEST

Blood sample for cytokine measurements and genetics.

ControlsCrohn's disease Patients

Up to 20 additional biopsies will be obtained at the time of standard of care colonoscopy. It is not felt that additional biopsies substantially increases the risk of a colonoscopy. Cytokine measurements will be performed on the tissue collected.

ControlsCrohn's disease Patients

To determine response, a Crohn's Disease Activity Index (CDAI) score will be obtained at time point 1 and time point 2 in all enrolled CD patients. The CDAI is a well validated symptom index for CD which assesses 8 factors including stool frequency, abdominal pain, general well being, CD complications, HCT, and deviation from ideal body weight.

Crohn's disease Patients

Blood sample for cytokine measurements after patients have been on therapy for at least 6 weeks but no more than 52 weeks.

Crohn's disease Patients

Up to 20 additional biopsies will be obtained at the time of standard of care colonoscopy at least 6 weeks but no more than 52 weeks after initiation of therapy. It is not felt that additional biopsies substantially increases the risk of a colonoscopy. Cytokine measurements will be performed on the tissue collected.

Crohn's disease Patients
Baseline stool sampleDIAGNOSTIC_TEST

Stool sample for inflammatory markers or microbiome analysis.

ControlsCrohn's disease Patients

Stool sample for inflammatory markers or microbiome analysis.

Crohn's disease Patients

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients who are scheduled for a colonoscopy at the Vanderbilt Endoscopy Laboratory as part of their routine care and who do not meet any of the pre-defined exclusion criteria will be considered for participation in the study as control subjects. Patients who are part of the Vanderbilt IBD practice who initiate therapy on an anti-TNF, ustekinumab, or vedolizumab as part of their routine care will be considered for participation in the study as subjects.

You may qualify if:

  • Patients that are seen in the Inflammatory Bowel Disease clinic with Crohn's disease who are initiating either treatment as part of their routine clinical care.
  • Healthy adults without IBD undergoing colonoscopy for colorectal cancer screening or other non-IBD related indication.

You may not qualify if:

  • pregnant
  • have a known coagulopathy or bleeding disorder
  • have known renal or hepatic impairment
  • have a history of organ transplantation
  • CD patients who are being seen in consultation and do not plan to receive longitudinal care through initiation their new therapy at the Vanderbilt IBD center will be excluded.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Inflammatory Bowel Disease Clinic, Vanderbilt University Medical Center

Nashville, Tennessee, 37212, United States

Location

Vanderbilt University Medical Center Endoscopy Laboratory

Nashville, Tennessee, 37232, United States

Location

Biospecimen

Retention: SAMPLES WITH DNA

Any remaining serum or tissue samples may be evaluated for other markers of IBD or stored for future studies.

MeSH Terms

Conditions

Inflammatory Bowel Diseases

Condition Hierarchy (Ancestors)

GastroenteritisGastrointestinal DiseasesDigestive System DiseasesIntestinal Diseases

Study Officials

  • Elizabeth Scoville, MD

    Vanderbilt University Medical Center

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

August 28, 2017

First Posted

August 30, 2017

Study Start

October 13, 2017

Primary Completion

May 3, 2022

Study Completion

May 3, 2022

Last Updated

April 14, 2023

Record last verified: 2022-05

Data Sharing

IPD Sharing
Will not share

Locations