NCT03258983

Brief Summary

Background: The plant-derived omega-3 fatty acid alpha-linolenic acid (ALA, 18:3-n-3) may reduce the risk of atherosclerotic cardiovascular disease, including incident myocardial infarction, ischemic stroke and peripheral artery disease. However, the results of previous studies have been inconsistent. Objectives: To investigate the associations between dietary intake of ALA, adipose tissue content of ALA, and the risk of the major atherosclerotic cardiovascular diseases incident myocardial infarction, ischemic stroke and subtypes, and peripheral artery disease. Methods: This project will be based on data from the Danish cohort study Diet, Cancer and Health which consisted of 57,053 men and women at recruitment between 1993 and 1997. Dietary intake of ALA will be assessed using a validated semiquantitative food-frequency questionnaire and adipose tissue content will be determined with the use of gas chromatography analyses of adipose tissue biopsies collected at baseline. Also, detailed information on lifestyle factors, medical history and anthropometri was collected at baseline. Incident cases have been identified through national registries and the diagnoses have previously been validated. Analyses of dietary intake of ALA will be analysed using a traditional cohort design, whereas analyses on adipose tissue content of ALA will be analysed based on a case-cohort design. Hazard ratioes with 95% confidence intervals will be used to describe the associations between the exposure variables and the outcome variables of interest.

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
57,053

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Nov 1993

Longer than P75 for all trials

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 24, 1993

Completed
19.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 10, 2013

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

July 10, 2013

Completed
4.1 years until next milestone

First Submitted

Initial submission to the registry

August 19, 2017

Completed
4 days until next milestone

First Posted

Study publicly available on registry

August 23, 2017

Completed
Last Updated

August 23, 2017

Status Verified

August 1, 2017

Enrollment Period

19.6 years

First QC Date

August 19, 2017

Last Update Submit

August 19, 2017

Conditions

Keywords

Alpha-linolenic acidDietary intakeAdipose tissueNutritional epidemiology

Outcome Measures

Primary Outcomes (3)

  • Incident acute myocardial infarction

    From baseline until July 2013

  • Incident ischemic stroke including total and subtypes of ischemic stroke

    From baseline until November 2009

  • Incident peripheral artery disease

    From baseline until December 2009

Secondary Outcomes (1)

  • Incident fatal myocardial infarction

    From baseline until July 2013

Study Arms (1)

The Diet, Cancer and Health cohort

Other: Alpha-linolenic acid

Interventions

Exposure to dietary intake of alpha-linolenic acid (ALA) will be assessed using a detailed food frequency questionaire filled in by participants included in the Diet, Cancer and Health cohort at baseline. Exposure to adipose tissue content of ALA will be determined by gas chromatography in all incident cases and in subcohort of 3500 participants randomly drawn from the total cohort.

Also known as: ALA (18:3-n3)
The Diet, Cancer and Health cohort

Eligibility Criteria

Age50 Years - 64 Years
Sexall
Age GroupsAdult (18-64)
Sampling MethodProbability Sample
Study Population

The Diet, Cancer and Health cohort was established over a period of 3.5 years between 1993 and 1997 by inviting 160,725 men and women to participate. All eligible participants were native Danish citizens without a previous diagnosis of cancer, aged 50-64 years, and living in the areas of Copenhagen and Aarhus. A total of 57053 accepted to participate in the Diet, Cancer and Health cohort study. A subcohort of 3500 participants were drawn randomly from the cohort at baseline.

You may qualify if:

  • Born in Denmark
  • Age between 50 and 64 years
  • Living in the areas of Copenhagen and Aarhus

You may not qualify if:

  • Previous cancer

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Publications (2)

  • Tjonneland A, Olsen A, Boll K, Stripp C, Christensen J, Engholm G, Overvad K. Study design, exposure variables, and socioeconomic determinants of participation in Diet, Cancer and Health: a population-based prospective cohort study of 57,053 men and women in Denmark. Scand J Public Health. 2007;35(4):432-41. doi: 10.1080/14034940601047986.

    PMID: 17786808BACKGROUND
  • Bork CS, Veno SK, Lundbye-Christensen S, Jakobsen MU, Tjonneland A, Schmidt EB, Overvad K. Dietary Intake of alpha-Linolenic Acid Is Not Appreciably Associated with Risk of Ischemic Stroke among Middle-Aged Danish Men and Women. J Nutr. 2018 Jun 1;148(6):952-958. doi: 10.1093/jn/nxy056.

Biospecimen

Retention: SAMPLES WITH DNA

Subcutaneous adipose tissue, serum, plasma, ''buffycoat'', erytrocytes and urine is stored in nitrogen vapour at the biobank of the Diet, Cancer and Health cohort study. Also, whole blood in filter paper and toenail clippings has been stored within the biobank.

MeSH Terms

Conditions

Ischemic StrokePeripheral Arterial Disease

Interventions

alpha-Linolenic Acid

Condition Hierarchy (Ancestors)

StrokeCerebrovascular DisordersBrain DiseasesCentral Nervous System DiseasesNervous System DiseasesVascular DiseasesCardiovascular DiseasesAtherosclerosisArteriosclerosisArterial Occlusive DiseasesPeripheral Vascular Diseases

Intervention Hierarchy (Ancestors)

Fatty Acids, Omega-3Dietary Fats, UnsaturatedDietary FatsFatsLipidsLinolenic AcidsFatty Acids, EssentialFatty Acids, UnsaturatedFatty Acids

Study Officials

  • Christian S Bork, MD

    Department of Cardiology, Aalborg University Hospital, Denmark

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Dr. Christian Sørensen Bork

Study Record Dates

First Submitted

August 19, 2017

First Posted

August 23, 2017

Study Start

November 24, 1993

Primary Completion

July 10, 2013

Study Completion

July 10, 2013

Last Updated

August 23, 2017

Record last verified: 2017-08