NCT03251183

Brief Summary

Heart failure (HF) currently affects app. 2% of the western population and app. 10% of people \>75 years. In about 50% of patients with symptomatic HF ejection fraction (EF) is preserved (HF-PEF). Once patients develop symptoms, the prognosis is poor with 25% mortality at 1 year and 50% mortality at 5 years. HFpEF is one of the major unresolved areas in clinical cardiology. The diagnosis of HFpEF remains a diagnosis of exclusion and currently no non-invasive measure provides a clear diagnosis. Cardiovascular magnetic resonance (CMR) provides non invasive and radiation free evaluation of heart structure and function. New CMR parameters offer the possibility to describe the underlying pathological and physiological changes associated with HFpEF. The investigators propose to undertake the first systematic comparison between a CMR protocol and invasive haemodynamics as the best possible gold standard, as well as define the histopathological drivers in myocardial biopsies. The investigators will also examine the relations with tissue and serological biomarkers implicated in HFpEF and the role with standard and novel parameters by echocardiography. If successful, this study will provide tools for a reliable and accurate non-invasive characterization of patients with HFpEF, supporting the diagnosis and grading the severity of disease. This study will provide a reference basis for future diagnostic algorithms in HFpEF, both, for CMR and echocardiography, but also for their relative value in comparison to blood markers or invasive testing. In addition to a new pathway to acess the effects of current and novel therapeutic interventions, the investigators see the greatest potential in identifying a disease stage where the myocardial injury may be reversible.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
185

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Jan 2018

Longer than P75 for all trials

Geographic Reach
1 country

7 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 14, 2017

Completed
3 months until next milestone

First Posted

Study publicly available on registry

August 16, 2017

Completed
5 months until next milestone

Study Start

First participant enrolled

January 14, 2018

Completed
5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2022

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2022

Completed
Last Updated

March 10, 2023

Status Verified

March 1, 2023

Enrollment Period

5 years

First QC Date

May 14, 2017

Last Update Submit

March 8, 2023

Conditions

Outcome Measures

Primary Outcomes (1)

  • Significant influence of MR Imaging Parameters on a multivariate model to describe the invasive pressure volume relations (EDPVR).

    Using a multivariable regression analysis and a respective F test.

    up to 4 weeks. No follow up is planned.

Secondary Outcomes (7)

  • Association between CMR T1-mapping and biopsy results.

    up to 4 weeks. No follow up is planned.

  • Association between CMR flow echocardiographic flow

    up to 4 weeks. No follow up is planned.

  • Association between a model for diastolic function based on CMR with a model of diastolic function based on echocardiography

    up to 4 weeks. No follow up is planned.

  • Association between CMR function and echocardiographic function

    up to 4 weeks. No follow up is planned.

  • Discriminatory capacity of a multivariate model of invasive and a multivariate model of non-invasive variables.

    up to 4 weeks. No follow up is planned.

  • +2 more secondary outcomes

Study Arms (4)

Main group

Blood sampling Comprehensive Cardiovascular magnetic resonance (CMR) Transthoracic echocardiography (TTE) (EchoErgo) Invasive pressure-volume (PV) Loops Left ventricular (LV) biopsy

Diagnostic Test: Comprehensive Cardiovascular magnetic resonance (CMR)Diagnostic Test: Blood samplingDiagnostic Test: TTE (EchoErgo)Diagnostic Test: Invasive pressure-volume (PV) LoopsDiagnostic Test: Left ventricular (LV) biopsy

Reproducibility group

Stress-perfusion Cardiovascular magnetic resonance (CMR)

Diagnostic Test: Comprehensive Cardiovascular magnetic resonance (CMR)Diagnostic Test: Blood sampling

Age/gender matched control group

Blood sampling Stress-perfusion Cardiovascular magnetic resonance (CMR) TTE (EchoErgo)

Diagnostic Test: Comprehensive Cardiovascular magnetic resonance (CMR)Diagnostic Test: Blood samplingDiagnostic Test: TTE (EchoErgo)

Healthy volunteers

Blood sampling Stress-perfusion Cardiovascular magnetic resonance (CMR) TTE (EchoErgo)

Diagnostic Test: Comprehensive Cardiovascular magnetic resonance (CMR)Diagnostic Test: Blood samplingDiagnostic Test: TTE (EchoErgo)

Interventions

Cardiovascular magnetic resonance (CMR) provides non-invasive, radiation-free and in-depth evaluation of myocardial structure and function. In addition to established tools for assessment of cardiac volume, mass, function and regional myocardial scar with late Gadolinium enhancement (LGE), several novel quantitative CMR parameters will be assessed including T1-mapping or fully quantitative perfusion Imaging.

Age/gender matched control groupHealthy volunteersMain groupReproducibility group
Blood samplingDIAGNOSTIC_TEST

Blood samples will eventually be analysed for markers related to heart failure (BNP/NT)-pro-BNP, myocardial inflammation and fibrosis (cytokine profiling, Galectin-3, Procollagen Type I and III, hsCRP). Whole blood will be frozen for DNA isolation and genome analysis. Peripheral blood mononuclear cells will be isolated by Ficoll in a subset of patients and will be used for RNA isolation allowing RNA-seq or reverse transcription (RT) - polymerase chain reaction (PCR) analysis.

Age/gender matched control groupHealthy volunteersMain groupReproducibility group
TTE (EchoErgo)DIAGNOSTIC_TEST

Measurements will include cavity dimensions, flow velocities, myocardial motion velocity and strain as well as for change of parameters during ergometric stress.

Age/gender matched control groupHealthy volunteersMain group

Multiple parameters (including EDPVR, ESPVR, dp/dt min, Tau, Ea) will be derived from the various PV loop assessments and additional relevant parameters will be calculated. Right ventricular and pulmonary pressures including pulmonary vascular resistance will be measured with Swan-Ganz catheters using right venous femoral approach.

Main group

A set of myocardial biopsies for each patient will be stained with Masson Trichrome for qualitative and quantitative assessment of the collagen volume fraction; fat droplets will be identified by red oil staining, Congo Red for amyloid immunohistology will be used to determine total leukocytes (CD45), T-cells (CD3) and monocytes/macrophages (CD68). A second set of biopsies will be frozen immediately and stored at -80°. Western blot analysis will be performed to determine alterations at the myofilament level including titin isoform composition and phosphorylation status.

Main group

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients with diagnostic criteria for HFpEF, age/gender matched controls and healty volunteers

You may qualify if:

  • Ability to provide informed consent
  • Typical HF symptoms (NYHA stage II-III) within the last 6 months
  • EF \> 45 % with absence of structural heart disease on echocardiography (except left ventricular hypertrophy or left atrial enlargement)
  • Echocardiographic evidence of increased left ventricular filling pressures
  • E/E'sep \>15 OR E/E'lat \>12 OR Av E/E' \>13 OR
  • E/E' \>9 AND left atrial (LA) volume \>34 ml/m2 OR systolic pulmonary artery pressure (PAsys): \>35 mmHg;
  • Indication for invasive hemodynamic work-up
  • Unclear aetiology of heart failure
  • Adults: age \>18 years

You may not qualify if:

  • Patients unable or unwilling to provide informed consent
  • High likelihood of non-diagnostic PV loops of MR imaging (e.g. atrial fibrillation or high rate of premature ventricular contraction (PVC) (\> 10 ventricular Extrasystole (VES)/minute), \> 150 kg body weight, inability to lie flat or still)
  • Contraindication for invasive work-up (allergy to contrast agent, severe renal insufficiency with estimated glomerular filtration rate (eGRF) \<30 ml/min)
  • Contraindications for a contrast enhanced CMR study (allergy to contrast agent, incompatible devices or implants (e.g. non-MR conditional pacemaker), severe claustrophobia)
  • Previous medical history of EF \<45%
  • Imaging findings confirming a specific diagnosis of myocardial impairment (e.g. amyloid, ischaemic heart disease, valvular disease)
  • Age-gender matched controls:
  • Ability to provide informed consent
  • No current or history of symptoms, signs or therapy for heart disease
  • EF \> 45 % with absence of structural heart disease on echocardiography (except left ventricular hypertrophy or left atrial enlargement)
  • Adults: age \>18 years
  • Patients unable or unwilling to provide informed consent
  • High likelihood of non-diagnostic MR imaging (e.g. atrial fibrillation or high rate of PVC (\> 10 VES/minute), \> 150 kg body weight, inability to lie flat or still)
  • Contraindications for a contrast enhanced CMR study (allergy to contrast agent, incompatible devices or implants (e.g. non-MR conditional pacemaker), severe claustrophobia, severe renal insufficiency with eGRF \<30 ml/min))
  • Previous medical history of EF \<45%
  • +10 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (7)

University Hospital Frankfurt

Frankfurt am Main, Hesse, 60590, Germany

Location

Kerckhoff Klinik

Bad Nauheim, Germany

Location

Charite Centrum Herz-, Kreislauf- und Gefäßmedizin

Berlin, Germany

Location

University Hospital Göttingen

Göttingen, Germany

Location

University Hospital

Heidelberg, Germany

Location

Herzzentrum Leipzig

Leipzig, Germany

Location

Uniersity Hospital Mainz

Mainz, Germany

Location

MeSH Terms

Interventions

Blood Specimen CollectionEchocardiographyBiopsy

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative TechniquesCardiac Imaging TechniquesDiagnostic ImagingUltrasonographyHeart Function TestsDiagnostic Techniques, CardiovascularCytodiagnosisCytological TechniquesDiagnostic Techniques, Surgical

Study Officials

  • Eike C Nagel, MD, PhD

    Goethe University Frankfurt

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Univ. Prof. Dr. med.

Study Record Dates

First Submitted

May 14, 2017

First Posted

August 16, 2017

Study Start

January 14, 2018

Primary Completion

December 31, 2022

Study Completion

December 31, 2022

Last Updated

March 10, 2023

Record last verified: 2023-03

Data Sharing

IPD Sharing
Will share

All data will be shared with other researchers within the German Centre for Cardiovascular Research (DZHK) via the Use and Access Rules

Shared Documents
STUDY PROTOCOL, ICF, CSR, ANALYTIC CODE
Time Frame
1 year after finalization of primary analysis
Access Criteria
Via Use and Access Policy of DZHK
More information

Locations