NCT03226015

Brief Summary

PURPOSE: To report clinical and histochemical results of oral mucosa graft transplantation in eyes with limbal stem cell deficiency. DESIGN: Prospective observational study. METHODS: 32 eyes of 27 patients with limbal stem cell deficiency underwent direct oral mucosa graft transplantation with amniotic membrane transplantation with a mean follow-up of 19 months. Clinical course of the disease including emergency surgeries, planned curative procedures, conjunctival inflammation, acute inpatient treatment and best corrected visual acuity were assessed at 3 months postoperatively and at last follow up visit. The unneeded parts of oral mucosa graft were analyzed immunohistochemically with staining for mesenchymal stem cell markers and pericytes (CD 90, CD 146, CD 166, CD 31, CD 68, protein gene product).

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
27

participants targeted

Target at below P25 for all trials

Timeline
Completed

Started Jul 2015

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

July 1, 2015

Completed
1.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 1, 2017

Completed
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2017

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

July 13, 2017

Completed
8 days until next milestone

First Posted

Study publicly available on registry

July 21, 2017

Completed
Last Updated

July 21, 2017

Status Verified

July 1, 2017

Enrollment Period

1.8 years

First QC Date

July 13, 2017

Last Update Submit

July 19, 2017

Conditions

Keywords

Limbal Stem-cell DeficiencyOral mucosa transplantationAmniotic membraneMesenchymal stem cells

Outcome Measures

Primary Outcomes (2)

  • change in number of acute surgeries

    baseline, 3 months after oral mucosa transplantation; through study completion, an average of 19 months

  • change in days of acute inpatient treatment

    baseline, 3 months after oral mucosa transplantation; through study completion, an average of 19 months

Secondary Outcomes (2)

  • change in number of curative surgical procedures after oral mucosa transplantation

    baseline, 3 months after oral mucosa transplantation; through study completion, an average of 19 months

  • change in conjunctival inflammation

    baseline, 3 months after oral mucosa transplantation; through study completion, an average of 19 months

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

All patients treated with oral mucosa with amniotic membrane transplantation in Klinikum Chemnitz 2015-2017

You may qualify if:

  • limbal stem cell deficiency at least in one eye
  • undergoing oral mucosa with amniotic membrane transplantation
  • obtained written informed consent

You may not qualify if:

  • no written informed consent

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Biospecimen

Retention: SAMPLES WITHOUT DNA

oral mucosa tissue

MeSH Terms

Conditions

Limbal Stem Cell Deficiency

Condition Hierarchy (Ancestors)

Corneal DiseasesEye Diseases

Study Officials

  • Katrin Engelmann, PhD

    Klinikum Chemnitz

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
CASE ONLY
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Head of Ophthalmology

Study Record Dates

First Submitted

July 13, 2017

First Posted

July 21, 2017

Study Start

July 1, 2015

Primary Completion

April 1, 2017

Study Completion

May 1, 2017

Last Updated

July 21, 2017

Record last verified: 2017-07