NCT03206359

Brief Summary

GADD34 (Growth Arrest and DNA Damage inducible-protein) is a regulatory subunit of PP1 (phosphatase 1) phosphatase which dephosphorylates eIF2alpha (eucaryotic initiation factor 2 alpha subunit), representing a negative feedback loop of the unfolded protein response (UPR). Moreover, GADD34 is necessary for type I interferon (IFN) production in response to viral infection in murine models. We investigate here the expression of GADD34 in systemic lupus erythematosus (SLE), in which type I IFN has an important pathogenic role. We report a case-control study on GADD34 gene expression in PBMC (peripheral blood mononuclear cells) of SLE patients (n=60) and age- and sex-matched healthy controls (n=30). The level of GADD34 gene expression, as well as of type-I IFN response genes in PBMC is measured by quantitative PCR.

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
90

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started Jan 2012

Typical duration for all trials

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 25, 2012

Completed
3.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 3, 2015

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 3, 2015

Completed
2.2 years until next milestone

First Submitted

Initial submission to the registry

June 30, 2017

Completed
2 days until next milestone

First Posted

Study publicly available on registry

July 2, 2017

Completed
Last Updated

July 5, 2017

Status Verified

June 1, 2017

Enrollment Period

3.2 years

First QC Date

June 30, 2017

Last Update Submit

July 3, 2017

Conditions

Keywords

Unfolded Protein ResponseGADD34Type I IFNIFN response genesType II IFNanti-DNA autoantibodies

Outcome Measures

Primary Outcomes (1)

  • GADD34 expression in SLE patients

    Evaluation of GADD34 expression in PBMC of patients suffering from SLE compared to GADD34 expression in PBMC of healthy subjets. GADD34 gene expression level is quantified in the PBMC by quantitative PCR

    4 years

Secondary Outcomes (4)

  • GADD34 and IFN response gene expression

    4 years

  • GADD34 expression and pathology activity

    4 years

  • GADD34 expression and pathology activity

    4 years

  • CHOP and BiP expression

    4 years

Study Arms (2)

Patients

Patients with SLE

Healthy subjects

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients suffering from systemic lupus erythematosus

You may qualify if:

  • Man and woman over 18 years old.
  • Patients suffering from SLE (SLICC criteria)
  • Patients having given their "Non-opposition"
  • Patients with social security affiliation (required in France)

You may not qualify if:

  • Patients suffering from other auto-immune disease (except SAPL, thyroiditis, and Gougerot Sjogren syndrome)
  • Patients with viral infection within 15 days
  • People with special protection (defined in articles L1121-§5-8 and articles L3212-§1-3 of French health care law)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

MeSH Terms

Conditions

Lupus Erythematosus, Systemic

Condition Hierarchy (Ancestors)

Connective Tissue DiseasesSkin and Connective Tissue DiseasesAutoimmune DiseasesImmune System Diseases

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 30, 2017

First Posted

July 2, 2017

Study Start

January 25, 2012

Primary Completion

April 3, 2015

Study Completion

April 3, 2015

Last Updated

July 5, 2017

Record last verified: 2017-06

Data Sharing

IPD Sharing
Will not share