NCT03182829

Brief Summary

This trial is conducted to assess the performance and handling of the in vitro diagnostic (IVD) device for oral direct factor Xa and thrombin inhibitors from urine samples of patients on treatment with direct oral anticoagulants Apixaban, Edoxaban, Rivaroxaban, and Dabigatran (DOAC) in an actual point-of-care (POCT) setting in comparison to results obtained by liquid chromatography tandem mass spectrometry (LC-MS/MS) from urine samples. This trial is conducted to assess the performance and handling of the IVD for oral direct factor Xa and thrombin inhibitors from urine samples of patients on treatment with DOACs in an actual point-of-care setting in comparison to results obtained by liquid chromatography tandem mass spectrometry (LC-MS/MS) from urine samples. "publication Thromb Haemost. 2019 Nov 8. doi: 10.1055/s-0039-1700545. \[Epub ahead of print\]"

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
880

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Aug 2018

Shorter than P25 for all trials

Geographic Reach
1 country

18 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 8, 2017

Completed
1 day until next milestone

First Posted

Study publicly available on registry

June 9, 2017

Completed
1.2 years until next milestone

Study Start

First participant enrolled

August 22, 2018

Completed
8 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 9, 2019

Completed
2 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 5, 2019

Completed
1.9 years until next milestone

Results Posted

Study results publicly available

May 4, 2021

Completed
Last Updated

April 21, 2022

Status Verified

March 1, 2022

Enrollment Period

8 months

First QC Date

June 8, 2017

Results QC Date

December 19, 2019

Last Update Submit

March 28, 2022

Conditions

Keywords

direct oral anticoagulantsapixabandabigatranedoxabanrivaroxabanurineLC-MS/MSevaluation studypoint of care testblood coagulationcoagulation testanticoagulantthrombosispulmonary embolismatrial fibrillationanticoagulationmass spectrometryPOC test

Outcome Measures

Primary Outcomes (1)

  • Accuracy of Factor Xa and Thrombin Inhibitors Pads of DOAC Dipstick From Urine Samples

    Liquid chromatography mass spectrometry versus DOAC Dipstick, qualitative analysis of results

    during urine collection and bioanalytical quantification, any time between August 2018 and April 2019

Study Arms (2)

Factor Xa inhibitor

Patients on treatment with Apixaban, Edoxaban or Rivaroxaban are included into the evaluation study at the outpatient care unit. Patients receive treatment for a specific clinical indication such as non-valvular atrial fibrillation or venous thromboembolism since one week or longer. During the study visit DOAC Dipstick test and liquid-chromatography mass-specrotmery are performed to identify absence or presence of Factor Xa inhibitor in urine. "publication Thromb Haemost. 2019 Nov 8. doi: 10.1055/s-0039-1700545. \[Epub ahead of print\]"

Diagnostic Test: DOAC Dipstick

Thrombin inhibitor

Patients on treatment with Dabigatran are included included into the evaluation study at the outpatient care unit. Patients receive treatment for a specific clinical indication such as non-valvular atrial fibrillation or venous thromboembolism since one week or longer. During the study visit DOAC Dipstick test and liquid-chromatography mass-specrotmery are performed to identify absence or presence of Thrombin inhibitor in urine. "publication Thromb Haemost. 2019 Nov 8. doi: 10.1055/s-0039-1700545. \[Epub ahead of print\]"

Diagnostic Test: DOAC Dipstick

Interventions

DOAC DipstickDIAGNOSTIC_TEST

Patients collect a sample of urine for analysis. "publication Thromb Haemost. 2019 Nov 8. doi: 10.1055/s-0039-1700545. \[Epub ahead of print\]"

Also known as: test strip for urine analysis"publication Thromb Haemost. 2019 Nov 8. doi: 10.1055/s-0039-1700545. [Epub ahead of print]"
Factor Xa inhibitorThrombin inhibitor

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodProbability Sample
Study Population

Patients on routine treatment with Apixaban, Dabigatran, Edoxaban, and Rivaroxaban since 1 week or longer, an age of \>18 years and fulfilling none the exclusion criteria are eligible. The patients participating in this Performance Assessment will be recruited directly at the point of care, i.e., the respective outpatient care unit. "publication Thromb Haemost. 2019 Nov 8. doi: 10.1055/s-0039-1700545. \[Epub ahead of print\]"

You may qualify if:

  • Fully signed and dated written informed consent
  • Age \>18 years
  • Patient is either under therapy with rivaroxaban, apixaban, and edoxaban or dabigatran for at least 1 week

You may not qualify if:

  • Patients not able to provide urine samples.
  • Patients not able to understand the informed consent or severe mentally disabled.
  • Patients in the end-stage of a severe disease.
  • "publication Thromb Haemost. 2019 Nov 8. doi: 10.1055/s-0039-1700545. \[Epub ahead of print\]"

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (18)

Universitätsherzzentrum Bad Krozingen, Klinik für Kardiologie und Angiologie II

Bad Krozingen, 79189, Germany

Location

Herz- und Diabeteszentrum NRW, Klinik für Kardiologie

Bad Oeynhausen, 32545, Germany

Location

Vivantes Klinikum im Friedrichshain

Berlin, 10249, Germany

Location

Gerinnungszentrum Sucker

Berlin, 10789, Germany

Location

Vivantes Klinikum Neukölln

Berlin, 12351, Germany

Location

Klinikum Coburg GmbH, II. Medizinische Klinik

Coburg, 96450, Germany

Location

Krankenhaus der Augustinerinnen gGmbH, Klinik für Kardiologie und internistische Intensivmedizin

Cologne, 50678, Germany

Location

Klinikum Darmstadt - Gefäßzentrum

Darmstadt, 64283, Germany

Location

Praxis Innere Medizin, Kardiologie und Angiologie

Dessau, 06846, Germany

Location

Städtisches Klinikum Dresden, II. Medizinische Klinik

Dresden, 01067, Germany

Location

Medizinische Fakultät Carl Gustav Carus, Medizinische Klinik und Poliklinik I

Dresden, 01307, Germany

Location

Cardioangiologisches Centrum Bethanien

Frankfurt, 60389, Germany

Location

Klinik für Kardiologie und Angiologie I

Freiburg im Breisgau, 79106, Germany

Location

Universitätsklinikum Hamburg-Eppendorf, II. Medizinische Klinik und Poliklinik

Hamburg, 20246, Germany

Location

Kliniken Landkreis Heidenheim

Heidenheim, 89522, Germany

Location

Zentrum für Blutgerinnungsstörungen, MVZ Labor Dr. Reising-Ackermann und Kollegen

Leipzig, 04289, Germany

Location

Zentrum für Praevention und Rehabilitation

Siegen, 57072, Germany

Location

Die Parkkardiologie

Stahnsdorf, 14532, Germany

Location

Related Publications (5)

  • Harenberg J, Du S, Wehling M, Zolfaghari S, Weiss C, Kramer R, Walenga J. Measurement of dabigatran, rivaroxaban and apixaban in samples of plasma, serum and urine, under real life conditions. An international study. Clin Chem Lab Med. 2016 Feb;54(2):275-83. doi: 10.1515/cclm-2015-0389.

    PMID: 26167981BACKGROUND
  • Du S, Weiss C, Christina G, Kramer S, Wehling M, Kramer R, Harenberg J. Determination of dabigatran in plasma, serum, and urine samples: comparison of six methods. Clin Chem Lab Med. 2015 Jul;53(8):1237-47. doi: 10.1515/cclm-2014-0991.

    PMID: 25720084BACKGROUND
  • Harenberg J, Du S, Kramer S, Weiss C, Kramer R, Wehling M. Patients' serum and urine as easily accessible samples for the measurement of non-vitamin K antagonist oral anticoagulants. Semin Thromb Hemost. 2015 Mar;41(2):228-36. doi: 10.1055/s-0035-1544158. Epub 2015 Feb 15.

    PMID: 25682081BACKGROUND
  • Harenberg J, Schreiner R, Hetjens S, Weiss C. Detecting Anti-IIa and Anti-Xa Direct Oral Anticoagulant (DOAC) Agents in Urine using a DOAC Dipstick. Semin Thromb Hemost. 2019 Apr;45(3):275-284. doi: 10.1055/s-0038-1668098. Epub 2018 Aug 22.

    PMID: 30134449BACKGROUND
  • Harenberg J, Beyer-Westendorf J, Crowther M, Douxfils J, Elalamy I, Verhamme P, Bauersachs R, Hetjens S, Weiss C; Working Group Members. Accuracy of a Rapid Diagnostic Test for the Presence of Direct Oral Factor Xa or Thrombin Inhibitors in Urine-A Multicenter Trial. Thromb Haemost. 2020 Jan;120(1):132-140. doi: 10.1055/s-0039-1700545. Epub 2019 Nov 8.

Related Links

Biospecimen

Retention: SAMPLES WITHOUT DNA

urine sample, spontaneously collected from patients "publication Thromb Haemost. 2019 Nov 8. doi: 10.1055/s-0039-1700545. \[Epub ahead of print\]"

MeSH Terms

Conditions

ThrombosisPulmonary EmbolismAtrial Fibrillation

Condition Hierarchy (Ancestors)

Embolism and ThrombosisVascular DiseasesCardiovascular DiseasesLung DiseasesRespiratory Tract DiseasesEmbolismArrhythmias, CardiacHeart DiseasesPathologic ProcessesPathological Conditions, Signs and Symptoms

Results Point of Contact

Title
Harenberg, Job Prof Dr.
Organization
DOASENSE GmbH

Study Officials

  • Job Harenberg, Professor

    Pneumonologische Schwerpunktpraxis Heidelberg, Privatpraxis Harenberg

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restriction Type
LTE60
Restrictive Agreement
Yes

Study Design

Study Type
observational
Observational Model
OTHER
Time Perspective
PROSPECTIVE
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 8, 2017

First Posted

June 9, 2017

Study Start

August 22, 2018

Primary Completion

April 9, 2019

Study Completion

June 5, 2019

Last Updated

April 21, 2022

Results First Posted

May 4, 2021

Record last verified: 2022-03

Data Sharing

IPD Sharing
Will not share

Individual data of patients are collected during the study and stored centrally at DOASENSE GmbH Heidelberg, Germany. They will be shared with other researchers upon request.

Locations