NCT03178747

Brief Summary

To compare the sensitivity of Tzanck smear with methylene blue stain versus traditional Giemsa stain in patients with herpes infection.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
150

participants targeted

Target at P25-P50 for phase_3

Timeline
Completed

Started Mar 2017

Shorter than P25 for phase_3

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 7, 2017

Completed
3 months until next milestone

First Submitted

Initial submission to the registry

June 2, 2017

Completed
5 days until next milestone

First Posted

Study publicly available on registry

June 7, 2017

Completed
6 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 7, 2017

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

March 6, 2018

Completed
Last Updated

August 4, 2017

Status Verified

August 1, 2017

Enrollment Period

9 months

First QC Date

June 2, 2017

Last Update Submit

August 3, 2017

Conditions

Keywords

Methylene blueGiemsaTzanck smear

Outcome Measures

Primary Outcomes (1)

  • Sensitivity

    Positive results from staining compared with number of cases

    24 weeks

Secondary Outcomes (2)

  • Factors that contribute the results from Methylene blue staining

    24 weeks

  • Specificity

    24 weeks

Study Arms (2)

Case

ACTIVE COMPARATOR

Patients who clinically diagnosed as herpes simplex, herpes zoster or varicella zoster skin infection.

Diagnostic Test: Methylene blueDiagnostic Test: Giemsa stain

Negative control

SHAM COMPARATOR

Patients who develops vesicular lesion but not typically considered as herpesviral skin infection, such as acute eczema, Paederus dermatitis

Diagnostic Test: Methylene blueDiagnostic Test: Giemsa stain

Interventions

Methylene blueDIAGNOSTIC_TEST

working solution methylene blue

CaseNegative control
Giemsa stainDIAGNOSTIC_TEST

working solution Giemsa stain (Wolbach's modification)

CaseNegative control

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • patients with clinically suspected of having herpes simplex, herpes zoster, varicella zoster skin infections.
  • Those who have vesicular skin lesion such as acute eczema, Paederus dermatitis.
  • Those are well cooperative.
  • Age \> 18 years old.

You may not qualify if:

  • Those who are not willing to have specimen from skin lesions to be collected.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Division of Dermatology, Faculty of Medicine, Chulalongkorn University

Bangkok, 10330, Thailand

RECRUITING

MeSH Terms

Conditions

Herpes SimplexHerpes Zoster

Condition Hierarchy (Ancestors)

Herpesviridae InfectionsDNA Virus InfectionsVirus DiseasesInfectionsSkin Diseases, ViralSkin Diseases, InfectiousSkin DiseasesSkin and Connective Tissue DiseasesVaricella Zoster Virus Infection

Study Officials

  • Pawinee Rerknimitr, MD, MSc

    Chulalongkorn University

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Pawinee Rerknimitr, MD, MSc

CONTACT

Study Design

Study Type
interventional
Phase
phase 3
Allocation
NON RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
DIAGNOSTIC
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assistant professor

Study Record Dates

First Submitted

June 2, 2017

First Posted

June 7, 2017

Study Start

March 7, 2017

Primary Completion

December 7, 2017

Study Completion

March 6, 2018

Last Updated

August 4, 2017

Record last verified: 2017-08

Data Sharing

IPD Sharing
Will not share

Locations