NCT03153787

Brief Summary

The objective of the present study to analyze the vaginal microbiome in patients who have not achieved gestation after oocyte donation treatment and after probiotic administration. All patients will receive vaginal probiotic according to the standard regimen. Sample collection will be performed the embryo transfer day and on the day of the pregnancy test. Follow up of gestation will be carried out. The analysis of the vaginal microbiome will be performed though massive genetic sequencing (determination of 16SRNA). The study variables, among others, are vaginal microbiome pattern after probiotic administration, the gestation rate, and the abortion rate.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
69

participants targeted

Target at P50-P75 for not_applicable

Timeline
Completed

Started Jun 2017

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 10, 2017

Completed
5 days until next milestone

First Posted

Study publicly available on registry

May 15, 2017

Completed
17 days until next milestone

Study Start

First participant enrolled

June 1, 2017

Completed
1.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 1, 2019

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2019

Completed
Last Updated

March 31, 2022

Status Verified

March 1, 2022

Enrollment Period

1.9 years

First QC Date

May 10, 2017

Last Update Submit

March 30, 2022

Conditions

Keywords

implantation failurefertilitymicrobiomeAssisted reproduction

Outcome Measures

Primary Outcomes (3)

  • Change from Baseline Vaginal microbiome at 2 and 4 weeks

    Vaginal microbiome will be analyzed through massive genetic sequencing (determination of 16S RRNA) Before and after probiotic administration

    2 weeks and 4 weeks

  • Gestation rate

    Gestation rate after assisted reproduction technique

    4 weeks

  • Abortion rate

    Abortion rate (clinical and biochemical) after assisted reproduction technique

    45 weeks

Secondary Outcomes (2)

  • Live newborn rate

    45 weeks

  • Complications during gestation

    45 weeks

Study Arms (1)

Not pregnancy

EXPERIMENTAL

Vaginal probiotic administration

Other: Probiotic

Interventions

Vaginal probiotic administration according to the standard regimen: 1 tablet (via vaginal) for 5 days after menstruation during the same cycle of the next embryo transfer.

Not pregnancy

Eligibility Criteria

Age19 Years - 49 Years
Sexfemale
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • Treated by oocyte donation.
  • Transfer of a single euploid embryo in the blastocyst phase.
  • \<3 months prior without antibiotic treatment.
  • Women who have completed the previous observational study "Study of the influence of vaginal and endometrial microbiome on the results of assisted reproduction techniques".
  • Women who have not achieved gestation after treatment.
  • Signed informed consent form.

You may not qualify if:

  • Uterine malformations.
  • Not treated hydrosalpinx
  • Known factors of implantation failure and repetitive abortions, such as haematological disorders of hypercoagulability.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Instituto Bernabeu

Alicante, 03016, Spain

Location

MeSH Terms

Interventions

Probiotics

Intervention Hierarchy (Ancestors)

Dietary SupplementsFoodDiet, Food, and NutritionPhysiological PhenomenaFood and Beverages

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
OTHER
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

May 10, 2017

First Posted

May 15, 2017

Study Start

June 1, 2017

Primary Completion

May 1, 2019

Study Completion

May 1, 2019

Last Updated

March 31, 2022

Record last verified: 2022-03

Data Sharing

IPD Sharing
Will not share

Locations