Estimation of Pentrexin-3 Levels in GCF and Saliva of Patients With Periodontal Disease

NCT03128502 | Completed

• 1 other identifier: PTX-3_AgP_CP
• Study Type: observational
• Target: 40
• Locations: 0 countries
• Sites: N/A

Brief Summary

Subjects were selected from the outpatient clinic, Department of Oral Medicine and Periodontology, Faculty of Oral and Dental Medicine, Cairo University. Ten healthy control subjects presented with clinically healthy periodontium. Ten patients who had plaque induced gingivitis. Ten patients who had generalized chronic periodontitis. Ten patients who had generalised aggressive periodontitis. Pentraxin-3 levels were evaluated in GCF and saliva of all subjects included in this study with ELISA technique.

Conditions

Chronic Periodontitis; Aggressive Periodontitis

Keywords

saliva; GCF; pentraxin-3

Outcome Measures

Primary Outcomes (1)

• GCF and salivary pentraxin-3 levels

  levels of pentraxin-3 in GCF and saliva

  baseline

Secondary Outcomes (4)

• plaque index

  baseline

• gingival index

  baseline

• probing pocket depth

  baseline

• clinical attachment level

  baseline

Study Arms (4)

healthy control

Ten healthy control subjects presented with clinically healthy periodontium. Control subjects had to meet the following criteria for inclusion: probing depth less than 3mm, no clinical attachment loss, no bleeding on probing, with Gingival and Plaque index 0-1.

plaque induced gingivitis

Ten patients who had plaque induced gingivitis characterized by the presence of any of the following clinical signs: redness and edema of the gingival tissue, bleeding upon provocation, changes in contour and consistency, presence of calculus and/or plaque, with no clinical attachment loss nor radiographic evidence of bone loss.

chronic periodontitis

Ten chronic moderate to severe periodontitis patients selected according to the criteria currently adopted by Armitage 1999. Moderate destruction is generally characterized by periodontal probing depths up to 6 mm with clinical attachment loss of up to 4 mm. Advanced destruction is generally characterized by periodontal probing depths greater than 6 mm with attachment loss greater than 4 mm. Radiographic evidence of bone loss is apparent, Increased tooth mobility may be present.

aggressive periodontitis

Ten patients suffering generalized aggressive periodontitis, patients were less than 35 years of age and had generalized interproximal attachment loss affecting at least 3 permanent teeth other than the first molars and incisors with at least one site each with PD and CAL \>5 mm, Attachment loss occurs in pronounced episodic periods of destruction, and there is familial aggregation (subjects were asked if they had at least one other member of the family presenting or with a history of periodontal diseases).

Eligibility Criteria

• Age: 25 Years - 50 Years
• Sex: all
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

Patients from the outpatient clinic, Department of Oral Medicine and Periodontology, Faculty of Oral and Dental Medicine, Cairo University suffering from diffrent periodontal diseases.

You may qualify if:

• patients with plaque induced gingivitis
• patients with chronic periodontitis
• patients with aggressive periodontitis

You may not qualify if:

• Patients with any systemic disease according to Cornell medical index (1968).
• Patients taking any medications or antibiotics in the last 3 months.
• Current or former smoker patients.
• Pregnant females.

Sponsors & Collaborators

• Cairo University: lead

Related Publications (3)

• Fujita Y, Ito H, Sekino S, Numabe Y. Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis. Odontology. 2012 Jul;100(2):215-21. doi: 10.1007/s10266-011-0042-1. Epub 2011 Sep 20.

  PMID: 21932007 RESULT

• Lakshmanan R, Jayakumar ND, Sankari M, Padmalatha O, Varghese S. Estimation of pentraxin-3 levels in the gingival tissues of chronic and aggressive periodontitis participants: an in vivo study. J Periodontol. 2014 Feb;85(2):290-7. doi: 10.1902/jop.2013.120718. Epub 2013 May 23.

  PMID: 23701480 RESULT

• Gumus P, Nizam N, Nalbantsoy A, Ozcaka O, Buduneli N. Saliva and serum levels of pentraxin-3 and interleukin-1beta in generalized aggressive or chronic periodontitis. J Periodontol. 2014 Mar;85(3):e40-6. doi: 10.1902/jop.2013.130281. Epub 2013 Aug 16.

  PMID: 23952075 RESULT

Biospecimen

Retention: SAMPLES WITHOUT DNA

salivary and GCF samples

MeSH Terms

Conditions

Chronic Periodontitis; Aggressive Periodontitis

Condition Hierarchy (Ancestors)

Periodontitis; Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Chronic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Officials

• Engy Ahmed, M.D.

  Associate Professor of Oral Medicine & Periodontology, faculty of Oral and Dental Medicine, Cairo University

  STUDY DIRECTOR

• Yasmine Mansour, B.D.Sc

  master candidate at faculty of dentistry cairo university

  PRINCIPAL INVESTIGATOR

• Olfat Shaker

  Professor of Medical Biochemistry, Faculty of Medicine, Cairo University

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University.

Study Record Dates

• First Submitted: April 21, 2017
• First Posted: April 25, 2017
• Study Start: December 1, 2015
• Primary Completion: February 1, 2017
• Study Completion: April 1, 2017
• Last Updated: June 15, 2017

Record last verified: 2017-06

Data Sharing

• IPD Sharing: Will not share