Effects of Dietary Nutrients on Liver and Adipose Tissue Metabolism

NCT03090347 | Unknown

• 1 other identifier: Oxlip-2017-BBSRC/BHF
• Study Type: interventional
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

In health,adipose tissue stores fat from the diet. If the fat tissue stops storing dietary fat then this increases the chance of it being stored in the liver, which is related to increased risk of heart disease and type 2 diabetes. It has been suggested that having a diet high in sugars or certain fats, may cause the fat tissue and liver to change how they function; we would like to investigate this in detail.

Conditions

NAFLD; Nutrient; Excess

Outcome Measures

Primary Outcomes (1)

• Liver de novo lipogenesis

  Investigators will utilise stable isotope tracer methodology to measure the change in the contribution of newly synthesised fatty acids to very low density lipoprotein triglyceride

  Within 21 days after starting the intervention diet

Secondary Outcomes (2)

• Adipose tissue metabolism

  Within 21 days after starting the intervention diet

• Liver fat accumulation

  Within 21 days after starting the intervention diet

Study Arms (1)

High-sugar diet

EXPERIMENTAL

Participants will be asked to consume a relatively low-fat, high-carbohydrate eucaloric diet enriched in free-sugars (20% total energy).

Other: Overconsumption of specific dietary nutrient

Interventions

Overconsumption of specific dietary nutrient OTHER

Subjects will be studied before and then after the consumption of a diet where specific nutrients are provided in excess.

High-sugar diet

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Participant is willing and able to give informed consent for participation in the study.
• Male or Female, aged ≥18 or ≤65 years.
• Body Mass Index ≥19 ≤35 kg/m2
• No medical condition or relevant drug therapy known to affect liver or adipose tissue metabolism.
• Weight stable for the previous 3 months

You may not qualify if:

• Patient is unwilling or unable to give informed consent for participation in the study
• A blood haemoglobin \<135mg/dL for men and \<120mg/dL for women
• Donated (or lost) ≥250 ml of blood in the previous two months.
• On a weight loss diet or have decreased their body weight by \>5% in the previous 3 months.
• Have increased their body weight by \>5% in the previous 3 months.
• Any metabolic condition or relevant drug therapy
• Current smoker
• History of alcoholism or a greater than recommended alcohol intake (\>30 g of alcohol daily for men and \>20 g of alcohol daily for women)
• Haemorrhagic disorders
• Anticoagulant treatment
• History of albumin allergy
• Pregnant or nursing mothers
• Women who are taking any contraceptive agent or device including oral contraceptives, hormone replacement therapy (HRT) or who have used these within the last 12 months
• History of severe claustrophobia
• Presence of metallic implants, pacemaker, or are unwilling to remove any piercings

Sponsors & Collaborators

• University of Oxford: lead

Study Sites (1)

Oxford Centre for Diabetes, Endocrinology and Metabolism

Oxford, OX3 7LE, United Kingdom

RECRUITING

MeSH Terms

Conditions

Non-alcoholic Fatty Liver Disease

Condition Hierarchy (Ancestors)

Fatty Liver; Liver Diseases; Digestive System Diseases

Central Study Contacts

Leanne Hodson, PhD

CONTACT

+441865857224; leanne.hodson@ocdem.ox.ac.uk

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 20, 2017
• First Posted: March 24, 2017
• Study Start: March 1, 2017
• Primary Completion: March 31, 2023
• Study Completion: March 31, 2023
• Last Updated: June 7, 2022

Record last verified: 2022-04

Data Sharing

• IPD Sharing: Will share

Locations

GB (1)