The Regulation of Human Skeletal Muscle Mass by Contractile Perturbation

NCT03046095 | Completed

• 1 other identifier: 02051986
• Study Type: interventional
• Target: 14
• Locations: 1 country
• Sites: 1

Brief Summary

It is well known that periods of weight training lead to increases in skeletal muscle size and strength. In contrast, periods of inactivity such as bed rest or immobilization result in losses of skeletal muscle size and strength. However, individuals experience variable magnitudes of muscle size change in response to changes in mechanical tension, such that certain individuals experience large changes in muscle mass whereas others do not. What is not currently known, and will be the primary goal of the present investigation, is to determine whether individuals who gain the most muscle mass with exercise training also lose the most muscle when they are immobilized. The investigators hypothesize that individuals who gain the most muscle with training will also lose the most with immobilization.

Conditions

Muscle Atrophy; Disuse Atrophy (Muscle) of Lower Leg

Keywords

Muscle Hypertrophy; Muscle Atrophy; Resistance Training; Immobilization

Outcome Measures

Primary Outcomes (2)

• Muscle Cross Sectional Area

  The changes in muscle cross sectional area will be assessed pre-training (week 0) and post training/immobilization (week 10) using magnetic resonance imaging (MRI). Muscle cross-sectional area will be assessed over a continuous period of 7 months.

  May 2017 - Dec 2017

• Leg Lean Mass

  The changes in leg lean mass will be assessed pre-training (week 0) and post-training/immobilization using dual energy x-ray absorptiometry. Leg lean mass measurements will be made over a continuous period of 7 months.

  May 2017 - Dec 2017

Secondary Outcomes (1)

• Skeletal Muscle Gene Expression

  May 2017 - Dec 2017

Study Arms (2)

Unilateral Resistance Exercise

EXPERIMENTAL

One of the participant's legs will be randomized to a unilateral resistance training arm for 10 weeks in duration. The leg chosen to be trained will undergo resistance exercise three days per week (Monday, Wednesday, and Friday) for the entirety of the study.

Other: Unilateral Resistance Exercise

Immobilization

EXPERIMENTAL

One of the participant's legs will be chosen to be immobilized during the last two weeks of the study. Therefore, one leg will be resistance exercising from week 0-10 whereas the other leg will be immobilized during weeks 8-10.

Procedure: Immobilization

Interventions

Unilateral Resistance Exercise OTHER

Unilateral Resistance exercise will include training three days per week and each session will include 3 sets of leg extension and 3 sets of leg press. In each set, the participant will complete a maximum of 12 repetitions.

Also known as: Resistance training

Unilateral Resistance Exercise

Immobilization PROCEDURE

During the last two weeks of the study (week 8-10), a Don Joy adjustable knee brace will be applied to the participant's leg randomized to immobilization. The brace will be applied at a 40 degree angle relative to complete extension.

Also known as: Knee Bracing

Immobilization

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy
• Non-Smoker
• Do not heavily consume alcohol

You may not qualify if:

• Female
• Younger than 18, or older than 30 years
• use of anti-inflammatory or analgesic medication
• history of neuromuscular disorders
• family history of deep vein thrombosis
• regularly take part in structured physical exercise (greater than 2 days per week)
• take any medications known to influence protein metabolism

Sponsors & Collaborators

• McMaster University: lead

Study Sites (1)

Exercise Metabolism Research Laboratory, McMaster Univeristy

Hamilton, Ontario, L8S 4K1, Canada

Location

MeSH Terms

Conditions

Muscular Atrophy; Muscular Disorders, Atrophic

Interventions

Resistance Training; Immobilization

Condition Hierarchy (Ancestors)

Neuromuscular Manifestations; Neurologic Manifestations; Nervous System Diseases; Atrophy; Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms; Signs and Symptoms; Muscular Diseases; Musculoskeletal Diseases; Neuromuscular Diseases

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Investigative Techniques

Study Officials

• Stuart M Phillips, PhD

  McMaster University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Masking Details: Investigators and participants will have knowledge or which leg will be undergoing resistance training and which will be immobilized.
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Model Details: The model will be a within-subject intervention. Each subject will have one leg randomized to unilateral resistance exercise (for 10 weeks) and the other to immobilization (for the last 2 weeks of the study).

Study Record Dates

• First Submitted: February 2, 2017
• First Posted: February 8, 2017
• Study Start: May 1, 2017
• Primary Completion: May 1, 2018
• Study Completion: September 1, 2018
• Last Updated: June 18, 2019

Record last verified: 2019-06

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)