NCT03043924

Brief Summary

The aim to evaluate whether activation of the hypothalamic-pituitary-gonadal axis in PCOS is associated with transient microstructural and metabolic changes in the female hypothalamus using MRI approaches to assess water diffusion and measure proton magnetic resonance spectra.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
52

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Sep 2017

Longer than P75 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

January 31, 2017

Completed
6 days until next milestone

First Posted

Study publicly available on registry

February 6, 2017

Completed
8 months until next milestone

Study Start

First participant enrolled

September 26, 2017

Completed
4.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 15, 2022

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 15, 2022

Completed
Last Updated

December 11, 2025

Status Verified

December 1, 2025

Enrollment Period

4.6 years

First QC Date

January 31, 2017

Last Update Submit

December 4, 2025

Conditions

Keywords

hypothalamusDiffusion Magnetic Resonance ImagingMagnetic Resonance Spectroscopy

Outcome Measures

Primary Outcomes (1)

  • Change in the Apparent Diffusion Coefficient (ADC)

    The change in the ADC will be analyzed in healthy volunteers and PCOS women before starting the oral contraceptives, and next during the third sequence of treatment.

    Baseline and 3 months after treatment start

Secondary Outcomes (5)

  • Change in the metabolite concentrations

    Baseline and 3 months after treatment start

  • Correlation coefficient (Spearman or Pearson) between the ADC and serum hormonal levels (FSH,luteinizing hormone,oestradiol, AMH)

    Baseline and 3 months after treatment start

  • Correlation coefficient (Spearman or Pearson) between the metabolite concentrations and serum hormonal levels (FSH, luteinizing hormone, oestradiol, AMH)

    Baseline and 3 months after treatment start

  • Plasma GnRH concentration before and after treatment (PCOS patient and control group)

    Baseline and 3 months after treatment start

  • Correlation between brain metabolite concentrations and plasma GnRH concentration before and after contraception.

    Baseline and 3 months after treatment start

Study Arms (2)

PCOS women

OTHER

26 PCOS women who receive consultation for hyperandrogenism needing treatment with Cyproterone Acetate + estradiol will be recruited.

Drug: Cyproterone Acetate + estradiol

Healthy volunteers

OTHER

26 Healthy volunteers subjects who receive consultation contemplating oral contraceptives (Levonorgestrel, Ethinyl Estradiol 0.1-0.02Mg Oral Tablet ) will be recruited.

Drug: Levonorgestrel, Ethinyl Estradiol 0.1-0.02Mg Oral Tablet

Interventions

Healthy volunteers receive combined oral contraceptive (COC) consisting 0.02 mg ethinylestradiol and 0.1mg of progestins (Leeloo Gé®)

Also known as: Leeloo Gé®
Healthy volunteers

PCOS will receive: 50mg cyproterone acetate (Androcur®) in combination with p.o. 2mg estradiol daily

Also known as: Androcur, Provames
PCOS women

Eligibility Criteria

Age19 Years - 30 Years
Sexfemale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • For all subjects
  • Aged from 19 to 30 years
  • Body mass index : 18-25
  • Do not smoke the 3 days before the MRI
  • For Healthy volunteers subjects :
  • Regular cycles (between 25 and 35 days) AND ovulatory.
  • No signs of hyperandrogenism.
  • Subjects desirous of taking oral contraceptives (estrogen/progestin combination).
  • For the realization of the 2nd MRI: to be during the 3rd month of a treatment well followed by oestro-progestative monophasic minidose to 0,02mg of Ethinyl-oestradiol / 0,1mg Levonorgestrel.
  • PCOS women:
  • PCOS defined by Rotterdam criteria
  • Needing a treatment with cyproterone acetate
  • To carry out the 2nd MRI: to be in progress during the 3rd month of a treatment well followed by 50 mg of Cyproterone acetate in association with 2mg / day of natural estradiol.

You may not qualify if:

  • On-going pregnancy (determined before each MRI scan visit)
  • Claustrophobia
  • Any metal or foreign implants (e.g., aneurysm clips, ear implants, heart pacemakers or defibrillators)
  • Diabetes or known dysthyroidism.
  • Current substance abuse (including smoking more than 5 cigarettes/day; determined using drug screening at the screening visit)
  • Pregnancy or breastfeeding the last 3 months.
  • Practice of intense physical exercise (ex jogging\> 10km) the day before the MRI.
  • Person incapable of consenting, or enjoying legal protection (guardianship / curatorship).
  • Unability to understand the treatment protocol

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Hôpital Jeanne de Flandres, CHU

Lille, France

Location

Related Publications (1)

  • Barbotin AL, Mimouni NEH, Kuchcinski G, Lopes R, Viard R, Rasika S, Mazur D, Silva MSB, Simon V, Boursier A, Pruvo JP, Yu Q, Candlish M, Boehm U, Bello FD, Medana C, Pigny P, Dewailly D, Prevot V, Catteau-Jonard S, Giacobini P. Hypothalamic neuroglial plasticity is regulated by anti-Mullerian hormone and disrupted in polycystic ovary syndrome. EBioMedicine. 2023 Apr;90:104535. doi: 10.1016/j.ebiom.2023.104535. Epub 2023 Mar 29.

MeSH Terms

Conditions

Polycystic Ovary Syndrome

Interventions

Cyproterone AcetateEstradiolLevonorgestrelEthinyl EstradiolTablets

Condition Hierarchy (Ancestors)

Ovarian CystsCystsNeoplasmsOvarian DiseasesAdnexal DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital DiseasesGonadal DisordersEndocrine System Diseases

Intervention Hierarchy (Ancestors)

CyproteronePregnadienesPregnanesSteroidsFused-Ring CompoundsPolycyclic CompoundsSteroids, ChlorinatedEstrenesEstranesEstradiol CongenersGonadal Steroid HormonesGonadal HormonesHormonesHormones, Hormone Substitutes, and Hormone AntagonistsNorgestrelNorpregnenesNorpregnanesNorsteroidsNorpregnatrienesEstrogenic Steroids, AlkylatedDosage FormsPharmaceutical Preparations

Study Officials

  • Didier Dewailly, MD,PhD

    University Hospital, Lille

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

January 31, 2017

First Posted

February 6, 2017

Study Start

September 26, 2017

Primary Completion

April 15, 2022

Study Completion

April 15, 2022

Last Updated

December 11, 2025

Record last verified: 2025-12

Data Sharing

IPD Sharing
Will not share

Locations