Control of Leukocyte Function

NCT03027232 | Completed

• 1 other identifier: 2007P000215
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

The protocol is to draw peripheral blood from healthy volunteers for in vitro studies. The aims of these in vitro studies are to determine the cellular and intracellular mechanisms by which hypertonic saline and ATP release regulate neutrophil and lymphocyte functions.

Conditions

Inflammation, Brain

Keywords

infection

Outcome Measures

Primary Outcomes (1)

• Immune cells will be studied in vitro to learn about basic mechanisms of immune cell signaling.

  The purpose of this study is to obtain blood from healthy subjects (volunteers) for in vitro studies to explore how immune cells function. Cells will be isolated and subjected to various tests including stimuli that simulate immune cell activation in health and disease. Subjects will not be subjected to any treatments. Only some of the cells (leukocytes) in the blood will be studied. For example, activation events such as cytokine production, the ability of leukocytes (=immune cells) to detect and respond to bacterial products will be assessed. No identifiable information about study subjects will be kept.

  Blood is collected as needed; time frame: indefinitely. Blood used between 1-7 days after blood draws.

Interventions

No intervention (drugs) will be given. Purpose: collecting blood for in vitro studies OTHER

No drugs or interventions are given. Single purpose is to draw blood from healthy subjects to study immune cell biology in vitro. No records about subjects to be kept.

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Normal healthy volunteers

You may qualify if:

• any healthy volunteer

You may not qualify if:

• pregnancy
• needing/using drugs
• inflammatory or infectious complications

Sponsors & Collaborators

• Beth Israel Deaconess Medical Center: lead

Study Sites (1)

BIDMC

Boston, Massachusetts, 02215, United States

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Blood is collected from all subjects in the same fashion. Purpose: isolation of immune cells for in vitro studies of cell function. This is an observational study in human beings in predefined groups of individuals who are healthy and volunteer to donate blood for laboratory science studies. Subjects in the study receive neither diagnostic, therapeutic, nor other interventions, and the investigator does not assign specific interventions to any of the subjects of the study.

MeSH Terms

Conditions

Encephalitis; Infections

Interventions

Pharmaceutical Preparations

Condition Hierarchy (Ancestors)

Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Neuroinflammatory Diseases

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor of Surgery

Study Record Dates

• First Submitted: January 13, 2017
• First Posted: January 23, 2017
• Study Start: January 1, 2007
• Primary Completion: December 15, 2017
• Study Completion: December 15, 2017
• Last Updated: April 27, 2018

Record last verified: 2018-04

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)