NCT02988011

Brief Summary

In this explorative randomized clinical study, the investigators aim to study metabolic, cellular, and molecular changes that occur during weight loss in obese subjects with and without type 2 diabetes. Using novel "imiomics" (imaging technique using PET/MR bioinformatics) analyses to examine possible metabolic differences between energy restricted diet and gastric by-pass surgery on whole-body and tissue specific insulin sensitivity, glucose tolerance, metabolite and protein profiles, fatty acid metabolism, ectopic fat content, and gene expression in adipose tissue. This study aims to identify novel biomarkers and drug targets for type 2 diabetes as well as validate promising and established biomarkers in an interventional model for improved glucose metabolism.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
50

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Feb 2016

Longer than P75 for not_applicable

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

February 28, 2016

Completed
9 months until next milestone

First Submitted

Initial submission to the registry

December 1, 2016

Completed
8 days until next milestone

First Posted

Study publicly available on registry

December 9, 2016

Completed
3.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2020

Completed
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2020

Completed
Last Updated

October 27, 2017

Status Verified

October 1, 2017

Enrollment Period

4.3 years

First QC Date

December 1, 2016

Last Update Submit

October 25, 2017

Conditions

Keywords

type 2 diabetesgastric by-pass surgerylow calorie dietglucose metabolism

Outcome Measures

Primary Outcomes (3)

  • Change in tissue specific glucose uptake measured by euglycemic clamp in PET/MR scanner

    Tissue specific assessment of glucose metabolism (18FDG uptake) using in-scanner (integrated PET-MR) insulin clamps.

    4 weeks

  • Metabolite concentrations in plasma

    Plasma metabolite concentrations (metabolomics) are assessed by liquid chromatography-mass spectrometry

    4 weeks

  • Gene expression in adipose tissue

    Change in gene expression is assessed by microarray (untargeted) messenger ribonucleic acid (mRNA) analyses

    4 weeks

Secondary Outcomes (5)

  • Lipoprotein changes in plasma assessed by routine clinical chemistry

    4 weeks

  • Plasma adipokines (e.g. adiponectin) assessed by elisa

    4 weeks

  • Fatty acid composition in plasma assessed by gas chromatography

    4 weeks

  • Changes in plasma protein profile (proteomics) assessed by immunohistochemistry

    4 weeks

  • Epigenetic changes assessed as DNA methylation

    4 weeks

Study Arms (2)

Gastric by-pass surgery

ACTIVE COMPARATOR

Gastric by-pass surgery without prior low-caloric diet

Procedure: Gastric by-pass surgery

Low-caloric diet

ACTIVE COMPARATOR

Low-caloric diet followed by gastric by-pass surgery

Procedure: Gastric by-pass surgeryDietary Supplement: Low-caloric diet

Interventions

Gastric by-pass surgery is expected to achieve a mean weight loss of 7-8kg in 4 weeks, which is similar weight-loss achieved in the other arm (low-caloric diet) during this time period.

Gastric by-pass surgeryLow-caloric diet
Low-caloric dietDIETARY_SUPPLEMENT

Energy restricted diet with a total energy intake of 800-1200kcal/day

Also known as: Energy restricted diet
Low-caloric diet

Eligibility Criteria

Age18 Years - 60 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • Age: 18-60 years
  • BMI 35-45
  • Sagittal abdominal diameter ≤38.5cm
  • For participants with type 2 Diabetes Mellitus, a disease duration of no more than 10 years, treated with 0-3 oral antidiabetic drugs and/or with diet. HbA1C 48-80mmol/mol at baseline visit.

You may not qualify if:

  • Diabetes complications: proliferative retinopathy, maculopathy, chronic renal failure stadium 3 with an eGFR\<60, foot ulcers, symptomatic neuropathy
  • Medications within 3 months: Insulin, Thiazolidinediones
  • Judgment by the investigator that the subject should not participate in the study if considers subject unlikely to comply with study procedures, restrictions and requirements
  • Pregnant or planning to be pregnant during the study.
  • Known or suspected history of significant drug abuse.
  • History of alcohol abuse or excessive intake of alcohol as judged by investigator.
  • History of severe allergy/hypersensitivity or ongoing allergy/hypersensitivity, as judged by the investigator
  • Sleep apnoea
  • Any previous serious cardiovascular event, stroke, acute myocardial infarction.
  • Any condition when MRI-PET is contraindicated such as, but not limited to, having a pacemaker or claustrophobia

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Uppsala univeristy hospital

Uppsala, 75185, Sweden

RECRUITING

Related Publications (1)

  • Eriksson JW, Pereira MJ, Kagios C, Kvernby S, Lundstrom E, Fanni G, Lundqvist MH, Carlsson BCL, Sundbom M, Tarai S, Lubberink M, Kullberg J, Riserus U, Ahlstrom H. Short-term effects of obesity surgery versus low-energy diet on body composition and tissue-specific glucose uptake: a randomised clinical study using whole-body integrated 18F-FDG-PET/MRI. Diabetologia. 2024 Jul;67(7):1399-1412. doi: 10.1007/s00125-024-06150-3. Epub 2024 Apr 24.

MeSH Terms

Conditions

Weight LossInsulin ResistanceNutrition DisordersGlucose Metabolism DisordersDiabetes Mellitus, Type 2

Interventions

Caloric Restriction

Condition Hierarchy (Ancestors)

Body Weight ChangesBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsHyperinsulinismMetabolic DiseasesNutritional and Metabolic DiseasesDiabetes MellitusEndocrine System Diseases

Intervention Hierarchy (Ancestors)

Diet TherapyNutrition TherapyTherapeuticsEnergy IntakeDietNutritional Physiological PhenomenaDiet, Food, and NutritionPhysiological Phenomena

Study Officials

  • Ulf Riserus, MMed, PhD

    Uppsala University

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Magnus Sundbom, MD, PhD

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate professor

Study Record Dates

First Submitted

December 1, 2016

First Posted

December 9, 2016

Study Start

February 28, 2016

Primary Completion

June 1, 2020

Study Completion

December 1, 2020

Last Updated

October 27, 2017

Record last verified: 2017-10

Locations