NCT02979210

Brief Summary

The rationale for the study is to better understand the dynamics of skin microflora following skin barrier disruption in an adult dermatitis model. The results from this study will provide insights in the complex interaction between skin microbiome and epidermis under normal and disease conditions

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
24

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Nov 2016

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2016

Completed
28 days until next milestone

First Submitted

Initial submission to the registry

November 29, 2016

Completed
2 days until next milestone

First Posted

Study publicly available on registry

December 1, 2016

Completed
2 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 26, 2017

Completed
6 days until next milestone

Study Completion

Last participant's last visit for all outcomes

February 1, 2017

Completed
Last Updated

August 18, 2017

Status Verified

August 1, 2017

Enrollment Period

3 months

First QC Date

November 29, 2016

Last Update Submit

August 15, 2017

Conditions

Outcome Measures

Primary Outcomes (2)

  • Type of microbes present on skin after exposure to artificial fecal insult

    Microbes will be identified by metagenomic sequencing

    up to 7 days

  • Relative abundance of microbes present on skin measured in picograms of DNA

    up to 7 days

Study Arms (1)

Artificial Fecal Insult

OTHER

protease/bile acid cocktail 200 microliters (1500 µg/ml trypsin/chymotrypsin protease mixture, 6.5 mg/ml cholic acid sodium, 6.2 mg/ml deoxycholic acid sodium, and 3.1 mg/ml chenodeoxycholic acid sodium in phosphate buffered saline)

Other: Artificial Fecal InsultOther: Phosphate Buffered Saline

Interventions

protease/bile acid cocktail 200 microliters (1500 µg/ml trypsin/chymotrypsin protease mixture, 6.5 mg/ml cholic acid sodium, 6.2 mg/ml deoxycholic acid sodium, and 3.1 mg/ml chenodeoxycholic acid sodium in phosphate buffered saline

Artificial Fecal Insult

200 microliters of phosphate buffered saline

Artificial Fecal Insult

Eligibility Criteria

Age18 Years - 40 Years
Sexfemale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy Subjects with Fitzpatrick Skin Types I, II or III

You may not qualify if:

  • Subjects with visible skin disease, tattoos, skin condition, or abnormal skin color

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Reliance Clinical Testing Service

Irving, Texas, 75062, United States

Location

Related Publications (2)

  • Costello EK, Lauber CL, Hamady M, Fierer N, Gordon JI, Knight R. Bacterial community variation in human body habitats across space and time. Science. 2009 Dec 18;326(5960):1694-7. doi: 10.1126/science.1177486. Epub 2009 Nov 5.

    PMID: 19892944BACKGROUND
  • Ishikawa J, Shimotoyodome Y, Ito S, Miyauchi Y, Fujimura T, Kitahara T, Hase T. Variations in the ceramide profile in different seasons and regions of the body contribute to stratum corneum functions. Arch Dermatol Res. 2013 Mar;305(2):151-62. doi: 10.1007/s00403-012-1286-5. Epub 2012 Sep 18.

    PMID: 22987221BACKGROUND

MeSH Terms

Conditions

Dermatitis

Condition Hierarchy (Ancestors)

Skin DiseasesSkin and Connective Tissue Diseases

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
BASIC SCIENCE
Intervention Model
SINGLE GROUP
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 29, 2016

First Posted

December 1, 2016

Study Start

November 1, 2016

Primary Completion

January 26, 2017

Study Completion

February 1, 2017

Last Updated

August 18, 2017

Record last verified: 2017-08

Locations