Impact of Neutrophil Extracellular Traps on Tissue Plasminogen Activator Induced Thrombolysis in Acute Ischemic Stroke Patients

NCT02907736 | Withdrawn

• 1 other identifier: JDS_2015_45
• Study Type: observational
• Target: N/A
• Locations: 1 country
• Sites: 1

Brief Summary

Acute Ischemic stroke (AIS) remains a leading cause of adult disability, cognitive impairment and mortality worldwide despite the development of revascularization therapies (intravenous Tissue Plasminogen Activator (t-PA) and endovascular therapy). Thrombosis resistance after IV t-PA therapy is frequent especially in case of AIS with proximal occlusion. In recent years, neutrophil extracellular traps (NETs) have been identified as major triggers and structural factors of various forms of thrombosis. NETs are extracellular webs primarily composed of DNA from neutrophils. A recent study shows that the NETs burden in coronary thrombi is positively correlated with the infarct size and negatively correlated with electrocardiogram (ST-segment) resolution. This later study revealed that in vitro addition of DNase I accelerated the t-PA-induced thrombolysis of coronary thrombi. NETs could, in consequence, be promising targets for improved thrombolysis in AIS. The aim of this study is to assess the impact of NETs composition of thrombi retrieved during endovascular therapy in AIS patients on IV t-PA induced thrombolysis, clinical outcome and AIS etiologies.

Conditions

Acute Stroke

Outcome Measures

Primary Outcomes (4)

• thrombus origin

  according to the etiology of the stroke (TOAST classification)

  baseline

• red cells counts

  quantitative assessment of the relative fractions of the main constituents after Hematoxylin and Eosin staining :

  baseline

• white cells counts

  quantitative assessment of the relative fractions of the main constituents after Hematoxylin and Eosin staining

  baseline

• platlet counts

  quantitative assessment of the relative fractions of the main constituents after Hematoxylin and Eosin staining

  baseline

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients with acute stroke, admitted for endovascular therapy.

You may qualify if:

• age \> 18 years old
• AIS secondary to a large vessel occlusion
• admitted for endovascular therapy.

You may not qualify if:

• Pregnant or breast feeding patient patient under legal protection
• Patient opposition to participate in this study

Sponsors & Collaborators

• Fondation Ophtalmologique Adolphe de Rothschild: lead

Study Sites (1)

Fondation Ophtalmologique Adolphe de Rothschild

Paris, 75019, France

Location

Related Publications (1)

• Ducroux C, Di Meglio L, Loyau S, Delbosc S, Boisseau W, Deschildre C, Ben Maacha M, Blanc R, Redjem H, Ciccio G, Smajda S, Fahed R, Michel JB, Piotin M, Salomon L, Mazighi M, Ho-Tin-Noe B, Desilles JP. Thrombus Neutrophil Extracellular Traps Content Impair tPA-Induced Thrombolysis in Acute Ischemic Stroke. Stroke. 2018 Mar;49(3):754-757. doi: 10.1161/STROKEAHA.117.019896. Epub 2018 Feb 8.

  PMID: 29438080 DERIVED

MeSH Terms

Conditions

Stroke

Condition Hierarchy (Ancestors)

Cerebrovascular Disorders; Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Vascular Diseases; Cardiovascular Diseases

Study Officials

• Jean-Philippe Désilles, MD

  Fondation OPH A. de Rothschild

  PRINCIPAL INVESTIGATOR

• Mikael Mazighi, MD, PhD

  Fondation OPH A. de Rothschild

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: NETWORK
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: September 6, 2016
• First Posted: September 20, 2016
• Study Start: November 12, 2015
• Primary Completion: July 1, 2017
• Study Completion: July 1, 2017
• Last Updated: March 19, 2018

Record last verified: 2018-03

Locations

FR (1)