Small RNA Pathways in Mammalian Gametogenesis

NCT02864329 | Completed

• 2 other identifiers: 12090130345P50HD076210
• Study Type: observational
• Target: 182
• Locations: 1 country
• Sites: 1

Brief Summary

Basic and clinical research is revealing that various noncoding and small RNAs play important and diverse roles in germ cell development and quality, including X/Y silencing during meiosis, gene regulation, DNA damage responses, and protection of the genome against transposable elements. Indeed, mammalian germ cells are known to harbor multiple small RNA species, including small interfering RNAs (siRNA), microRNAs (miRNA), and germline- specific PIWI- interacting RNAs (piRNA). However, their mechanistic roles in gametogenesis and human infertility are largely uncharacterized. The goal of this study is to elucidate the role of small RNA pathways in the events that give rise to viable euploid gametes. Four projects and three cores are included in this study.

Conditions

Male Infertility

Keywords

Male Infertility; micro RNAs; small interfering RNAs; miRNA; siRNA

Outcome Measures

Primary Outcomes (1)

• Using RNA from men with normal spermatogenesis and men with infertility for multiplexed deep sequencing to identify differentially expressed miRNAs

  Detect target mRNAs through correlation analysis of actual mRNA expression profiles obtained from the same patients. At the end of the proposed funding period, project 2 will have identified and confirmed a set of approximately 20-30 miRNA:mRNA interactions in male infertility.

  5 years

Eligibility Criteria

• Age: 18 Years - 90 Years
• Sex: male
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Men between the age of 18 and 90 who have testicular cancer and underwent surgery for removal at Weill Cornell Medicine.

You may qualify if:

• Men between the ages of 18-90 who have testicular cancer and underwent surgery at Weill Cornell Medicine.

You may not qualify if:

• Women, men outside of the age parameters

Sponsors & Collaborators

• Weill Medical College of Cornell University: lead
• Cornell University: collaborator
• Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD): collaborator

Study Sites (1)

Weill Cornell Medicine

New York, New York, 10065, United States

Location

Biospecimen

Retention: SAMPLES WITH DNA

Testicular tissue, penile tissue

MeSH Terms

Conditions

Infertility, Male

Condition Hierarchy (Ancestors)

Genital Diseases, Male; Genital Diseases; Urogenital Diseases; Infertility; Male Urogenital Diseases

Study Officials

• Peter N Schlegel, M.D.

  Weill Medical College of Cornell University

  PRINCIPAL INVESTIGATOR

• Paula E. Cohen, Ph.D.

  Cornell University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: April 26, 2016
• First Posted: August 12, 2016
• Study Start: April 1, 2014
• Primary Completion: March 20, 2024
• Study Completion: March 20, 2024
• Last Updated: July 19, 2024

Record last verified: 2024-07

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)