Effect of NO Precursors on Physiological Responses to Exercise

NCT02850367 | Completed

• 1 other identifier: 38RC14.430
• Study Type: interventional
• Target: 63
• Locations: 1 country
• Sites: 1

Brief Summary

NO is an ubiquitous molecule contributing to many physiological functions such as vasodilation, immune and oxidative stress responses. NO production can be supplemented either by providing substrate to the NO-synthase (L-Arginine and L-Citruline) or by providing nitrate which can lead to NO. In the present project the investigators evaluate the effect of various acute and chronic oral NO precursors supplementation on exercise performance and responses in healthy young and older subjects.

Conditions

Physiology

Outcome Measures

Primary Outcomes (2)

• Neuromuscular evaluations

  After the intake of the supplement. Measure the stretching force in the ankle (N)

  3 half days

• Evaluation of post-ischemic vascular reactivity

  After the intake of the supplement. Concentration of hemoglobin (g/mL) in the quadriceps with the device : NIRS system Oxymon, ARTINIS.

  3 half days

Study Arms (2)

Acute intake

ACTIVE COMPARATOR

Evaluation after acute intake of the food supplement.

Dietary Supplement: Nitrate; Dietary Supplement: Nitrate and L-Arginine; Dietary Supplement: Nitrate and L-Citrulline; Dietary Supplement: Placebo; Biological: Evaluation of post-ischemic vascular reactivity; Biological: Neuromuscular evaluations

Chronic intake

ACTIVE COMPARATOR

Evaluation before and after chronic intake of the food supplement.

Dietary Supplement: Nitrate and L-Citrulline; Dietary Supplement: Placebo; Biological: Evaluation of post-ischemic vascular reactivity; Biological: Neuromuscular evaluations

Interventions

Evaluation of post-ischemic vascular reactivity BIOLOGICAL

After the intake of the supplement. Concentration of hemoglobin (g/mL) in the quadriceps with the device : NIRS system Oxymon, ARTINIS.

Acute intake; Chronic intake

Nitrate DIETARY_SUPPLEMENT

Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.

Acute intake

Nitrate and L-Arginine DIETARY_SUPPLEMENT

Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.

Acute intake

Nitrate and L-Citrulline DIETARY_SUPPLEMENT

Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.

Acute intake; Chronic intake

Placebo DIETARY_SUPPLEMENT

Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with a placebo.

Acute intake; Chronic intake

Neuromuscular evaluations BIOLOGICAL

After the intake of the supplement. Measure the stretching force in the ankle (N)

Acute intake; Chronic intake

Eligibility Criteria

• Age: 18 Years - 70 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Sedentary subjects
• No cardiovascular, respiratory, metabolic and neurological disorders

You may not qualify if:

• Body mass index \> 28 kg.m-2
• Smoker
• Alcool intake \> 10g/day
• Psychiatric diseases
• Subjects under treatment able to interfere with their cardiovascular, metabolic and neurological status

Sponsors & Collaborators

• University Hospital, Grenoble: lead

Study Sites (1)

Grenoble University Hospital

Grenoble, Auvergne-Rhône-Alpes, 38043, France

Location

Related Publications (37)

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• LE Roux-Mallouf T, Vallejo A, Pelen F, Halimaoui I, Doutreleau S, Verges S. Synergetic Effect of NO Precursor Supplementation and Exercise Training. Med Sci Sports Exerc. 2020 Nov;52(11):2437-2447. doi: 10.1249/MSS.0000000000002387.

  PMID: 33064413 RESULT

MeSH Terms

Interventions

Nitrates; Arginine; Citrulline

Intervention Hierarchy (Ancestors)

Anions; Ions; Electrolytes; Inorganic Chemicals; Nitric Acid; Nitrogen Compounds; Organic Chemicals; Amino Acids, Basic; Amino Acids; Amino Acids, Peptides, and Proteins; Amino Acids, Diamino; Amino Acids, Essential

Study Officials

• Stéphane Doutreleau, PhD

  University Hospital, Grenoble

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: May 18, 2016
• First Posted: August 1, 2016
• Study Start: May 9, 2016
• Primary Completion: September 1, 2021
• Study Completion: September 1, 2021
• Last Updated: December 29, 2025

Record last verified: 2025-12

Data Sharing

• IPD Sharing: Will not share

Locations

FR (1)