Effects of Hypoxia and Inflammation on Citrulline Synthesis by Ornithine Transcarbamylase in Human Enterocytes

NCT02820064 | Unknown

• 1 other identifier: 38RC15.303
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

Chronic Obstructive Pulmonary Disease (COPD) is characterized by chronic systemic hypoxia and low-grade inflammation as well as by an alteration of arginine (ARG) metabolism. As ARG is synthetized from circulating citrulline (CIT), an alteration of CIT homeostasis, particularly its production by ornithine transcarbamylase (OCT) in small intestine could be involved. We hypothesized that hypoxia +/- inflammation, classically associated to COPD, has effects on OCT regulation in enterocytes. This study aims at exploring the effects of hypoxia and inflammation on the production of citrulline by ornithine transcarbamylase (OTC) activity in enterocytes from explant cultures of duodenal tissue.

Conditions

Hypoxia; Inflammation; Chronic Obstructive Pulmonary Disease

Outcome Measures

Primary Outcomes (1)

• OCT activity in biopsy specimens

  OCT activity in duodenal biopsy specimens will be measured at the end of the incubation period and compared between the 4 different groups (standard/inflammatory/hypoxic:inflammatory+hypoxic conditions)

  OCT activity in biopsies after incubation

Study Arms (1)

only one arm

OTHER

Patients for who and esophagogastroduodenoscopy in order to diagnose is performed. 8 duodenal biopsy specimens will be removed.

Procedure: duodenal biopsy during gastroduodenal endoscopy

Interventions

duodenal biopsy during gastroduodenal endoscopy PROCEDURE

Patients for who and esophagogastroduodenoscopy in order to diagnose is performed. 8 duodenal biopsy specimens will be removed.

only one arm

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Digestive disease known or suspected which justify an oeso-gastro-endoscopy with digestive biopsies, excepted a disease with duodenal localization known or strongly suspected.
• Gastroscopy performed under general anaesthesia
• Patient's written agreement obtained
• Age \< 18
• Therapeutical endoscopy (that is to say when a therapeutical act will be performed as balloon dilation, digestive or biliary prosthesis, drainage, diverticulotomy, polypectomy, variceal ligation...) either expected or
• Duodenal pathology (known or strongly suspected with the clinical and biological elements)
• Duodenal biopsies are not allowed to be performed: vascular lesions, digestive haemorrhage, clotting disorder.
• Antiplatelet drug and anticoagulant drug
• Lack of written agreement
• Subjects hospitalized in an emergency state or without agreement
• Subjects who cannot be contacted in emergency.

You may not qualify if:

• subjects will be excluded from the study:
• if an unexpected therapeutic act has to be performed during the endoscopy
• if duodenal duodenal atrophy or lesions are discovered during the endoscopy
• if duodenal lesions are discoverd at the histological examination

Sponsors & Collaborators

• University Hospital, Grenoble: lead
• AGIR à Dom: collaborator

Study Sites (1)

Grenoble University Hospital

Grenoble, 38043, France

RECRUITING

Related Publications (31)

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MeSH Terms

Conditions

Hypoxia; Inflammation; Pulmonary Disease, Chronic Obstructive

Condition Hierarchy (Ancestors)

Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Pathologic Processes; Lung Diseases, Obstructive; Lung Diseases; Respiratory Tract Diseases; Chronic Disease; Disease Attributes

Study Officials

• Eric Fontaine, Professor

  Division of clinical Nutrition-Grenoble University Hospital

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Eric FONTAINE, Professor

CONTACT

efontaine@chu-grenoble.fr

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: June 28, 2016
• First Posted: June 30, 2016
• Study Start: January 1, 2016
• Primary Completion: January 1, 2017
• Study Completion: January 1, 2017
• Last Updated: December 6, 2016

Record last verified: 2016-12

Data Sharing

• IPD Sharing: Will not share

Locations

FR (1)