Study of Collagen IV and XIX in Bronchoalveolar Lavage, Pulmonary Aspiration and Bronchial Biopsies

NCT02788643 | Completed

• 1 other identifier: PJ13017
• Study Type: observational
• Target: 51
• Locations: 1 country
• Sites: 1

Brief Summary

Collagens are proteins present in all tissues. Besides their structural role, recent data showed that they were able to regulate many cellular functions. Many interactions occur between extracellular matrix macromolecules, especially collagen, and various cell types. These interactions can be controlled by peptides derived extracellular matrix macromolecules, called matrikines. Previous work from the investigators laboratory and others have shown that several C-terminal domain (NC1 domains) from basement membrane-associated collagens could regulate many cellular activities. Lung is an organ particularly abundant in basement membranes. It is likely that various lung diseases may affect metabolism of basement membrane associated collagens. To the investigators knowledge, no study has focused on the expression of collagen XIX α1 chain and collagen IV chains α3 and α4 chains in lung and studied possible variations of expression in various pathophysiological situations. The aim of this study are to:

• Study the presence of collagen IV and XIX (or fragments) in different types of sampling such as bronchoalveolar lavage, pulmonary aspiration and bronchial biopsies
• Evaluate quantitative variations of expression of these collagen in different pulmonary diseases, especially chronic obstructive bronchopneumopathy, infectious pneumonia, pneumonitis or lung cancer.

Conditions

Chronic Obstructive Bronchopneumopathy; Infectious Pneumonia; Pneumonitis; Lung Cancer

Outcome Measures

Primary Outcomes (2)

• Collagen expression

  western blot and Reverse transcription polymerase chain reaction

  9 months

• Collagen expression

  western blot and Reverse transcription polymerase chain reaction

  15 months

Interventions

biological fluids (serum, bronchoalveolar lavage and pulmonary aspiration) and bronchial biopsies collection BIOLOGICAL

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Not relevant

You may qualify if:

• Patient from the division of pulmonary medicine of the Reims University Hospital
• Patient that must undergo a bronchoscopy with bronchoalveolar lavage
• Male or female
• Informed of the study (written information) and not objecting to it (oral agreement)

You may not qualify if:

• \- Aged bellow 18 years

Sponsors & Collaborators

• CHU de Reims: lead

Study Sites (1)

Chu Reims

Reims, 51092, France

Location

Related Publications (1)

• Dupont-Deshorgue A, Oudart JB, Brassart B, Deslee G, Perotin JM, Diebold MD, Monboisse JC, Ramont L, Brassart-Pasco S. A competitive enzyme-linked immunosorbent assay for quantification of tetrastatin in body fluids and tumor extracts. Anal Biochem. 2015 Aug 1;482:16-21. doi: 10.1016/j.ab.2015.04.023. Epub 2015 Apr 29.

  PMID: 25935259 BACKGROUND

MeSH Terms

Conditions

Pulmonary Disease, Chronic Obstructive; Pneumonia; Lung Neoplasms

Interventions

Bronchoalveolar Lavage

Condition Hierarchy (Ancestors)

Lung Diseases, Obstructive; Lung Diseases; Respiratory Tract Diseases; Chronic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Respiratory Tract Infections; Infections; Respiratory Tract Neoplasms; Thoracic Neoplasms; Neoplasms by Site; Neoplasms

Intervention Hierarchy (Ancestors)

Therapeutic Irrigation; Investigative Techniques

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: May 20, 2016
• First Posted: June 2, 2016
• Study Start: May 1, 2014
• Primary Completion: December 1, 2017
• Study Completion: December 1, 2017
• Last Updated: May 13, 2021

Record last verified: 2021-05

Locations

FR (1)