NCT02782351

Brief Summary

The present study evaluates the safety and efficacy of humanized Chimeric antigen receptor T cells (CAR-T) in treating recurrent or refractory B cell malignancy targeting CD19 with a humanized scFv. All participants will receive autologous chimeric antigen receptor engineered T cells.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
50

participants targeted

Target at P50-P75 for phase_1

Timeline
Completed

Started May 2016

Typical duration for phase_1

Geographic Reach
1 country

2 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

May 1, 2016

Completed
16 days until next milestone

First Submitted

Initial submission to the registry

May 17, 2016

Completed
8 days until next milestone

First Posted

Study publicly available on registry

May 25, 2016

Completed
2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2018

Completed
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2018

Completed
Last Updated

August 4, 2017

Status Verified

August 1, 2017

Enrollment Period

2.1 years

First QC Date

May 17, 2016

Last Update Submit

August 3, 2017

Conditions

Outcome Measures

Primary Outcomes (1)

  • CAR-T cells persistence in peripheral blood

    The presence of CAR T cells in patients' peripheral blood will be quantified with real time qPCR

    12 months

Secondary Outcomes (1)

  • B cell number and immunoglobulins in peripheral blood

    12 months

Study Arms (1)

CAR-T

EXPERIMENTAL

In interventional studies, patients enrolled will receive autologous 2nd generation CAR-T cells, which contain a humanized single chain antibody sequence against CD19.

Biological: CAR-T

Interventions

CAR-TBIOLOGICAL

Patients will be infused with autologous CAR-T infusion in a dose escalating manner.

CAR-T

Eligibility Criteria

Age3 Years+
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Age≥3 at the time of consent
  • Survival time\>12 weeks
  • B cell hematological malignancies by pathological examination
  • Chemotherapy failure or recurrent B cell malignancy
  • Creatinine\< 2.5mg/dl
  • Glutamic-pyruvic transaminase, glutamic oxalacetic transaminase\< 3 fold of normal level
  • Karnofsky Performance Status\>50% at the time of screening
  • Bilirubin\<2.0mg/dl
  • Adequate pulmonary, renal, hepatic, and cardiac function
  • Fail in autologous or allogenic haemopoietic stem cell transplantation
  • Free of leukocytes removal contraindications

You may not qualify if:

  • Pregnant or nursing women
  • Active hepatitis B, active hepatitis C, or any human immunodeficiency virus (HIV) infection at the time of screening
  • Previous treatment with any gene therapy product
  • Abnormal vital signs
  • Highly allergic constitution or history of severe allergies, especially allergy to interleukin-2
  • General infection or local severe infection, or other infection that is not controlled
  • Dysfunction in lung, heart, kidney and brain.
  • Severe autoimmune diseases
  • other symptoms that are not applicable for CAR-T

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Huaian First People's Hospital

Huai'an, Jiangsu, 223300, China

RECRUITING

Affiliated hospital of Xuzhou medical college

Xuzhou, Jiangsu, 221000, China

RECRUITING

Related Publications (4)

  • van der Stegen SJ, Hamieh M, Sadelain M. The pharmacology of second-generation chimeric antigen receptors. Nat Rev Drug Discov. 2015 Jul;14(7):499-509. doi: 10.1038/nrd4597.

    PMID: 26129802BACKGROUND
  • Davila ML, Bouhassira DC, Park JH, Curran KJ, Smith EL, Pegram HJ, Brentjens R. Chimeric antigen receptors for the adoptive T cell therapy of hematologic malignancies. Int J Hematol. 2014 Apr;99(4):361-71. doi: 10.1007/s12185-013-1479-5. Epub 2013 Dec 6.

  • Qi Y, Zhao M, Hu Y, Wang Y, Li P, Cao J, Shi M, Tan J, Zhang M, Xiao X, Xia J, Ma S, Qiao J, Yan Z, Li H, Pan B, Sang W, Li D, Li Z, Zhou J, Huang H, Liang A, Zheng J, Xu K. Efficacy and safety of CD19-specific CAR T cell-based therapy in B-cell acute lymphoblastic leukemia patients with CNSL. Blood. 2022 Jun 9;139(23):3376-3386. doi: 10.1182/blood.2021013733.

  • Chen W, Ma Y, Shen Z, Chen H, Ma R, Yan D, Shi M, Wang X, Song X, Sun C, Cao J, Cheng H, Zhu F, Sun H, Li D, Li Z, Zheng J, Xu K, Sang W. Humanized Anti-CD19 CAR-T Cell Therapy and Sequential Allogeneic Hematopoietic Stem Cell Transplantation Achieved Long-Term Survival in Refractory and Relapsed B Lymphocytic Leukemia: A Retrospective Study of CAR-T Cell Therapy. Front Immunol. 2021 Oct 29;12:755549. doi: 10.3389/fimmu.2021.755549. eCollection 2021.

MeSH Terms

Conditions

Leukemia, Lymphocytic, Chronic, B-Cell

Condition Hierarchy (Ancestors)

Leukemia, B-CellLeukemia, LymphoidLeukemiaNeoplasms by Histologic TypeNeoplasmsHematologic DiseasesHemic and Lymphatic DiseasesLymphoproliferative DisordersLymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesChronic DiseaseDisease AttributesPathologic ProcessesPathological Conditions, Signs and Symptoms

Study Officials

  • KaiLin Xu, MD. Ph.D.

    Xuzhou Medical University

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Jiang Cao, M.D., Ph.D.

CONTACT

JunNian Zheng, M.D., Ph.D.

CONTACT

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
President

Study Record Dates

First Submitted

May 17, 2016

First Posted

May 25, 2016

Study Start

May 1, 2016

Primary Completion

June 1, 2018

Study Completion

December 1, 2018

Last Updated

August 4, 2017

Record last verified: 2017-08

Data Sharing

IPD Sharing
Will share

Locations