Impact of Ageing on Adipose, Muscle and Systemic Inflammation

NCT02777138 | Unknown

• 1 other identifier: 16/sw/0003
• Study Type: observational
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

The accumulation and dysfunction of excess adipose (fat) tissue that occurs with ageing is associated with a number of chronic inflammatory disorders such as type 2 diabetes and cardiovascular disease but the underlying mechanisms are not understood.

Conditions

Inflammation; Aging

Keywords

Adipose tissue; Inflammation; Age Comparison; Macrophages; T-lymphocytes; Adipose Progenitors

Outcome Measures

Primary Outcomes (1)

• Differences in immune cell populations (macrophages/Tcells) in adipose tissue of young versus old males

  through to study completion, an average of 14 months

Secondary Outcomes (5)

• the impact of ageing on mRNA expression of key metabolic and inflammatory genes in adipose tissue.

  through to study completion, an average of 14 months

• the impact of ageing on metabolic and inflammatory protein secretions (into culture media) from adipose tissue

  through to study completion, an average of 14 months

• differences in immune cell populations in muscle tissue and blood compared to adipose tissue from the same individuals and the impact of ageing on immune cell populations in muscle tissue.

  through to study completion, an average of 14 months

• the impact of ageing on mRNA expression of other key metabolic and inflammatory genes in muscle tissue.

  through to study completion, an average of 14 months

• activation/inhibition of e.g. insulin stimulated pathways in adipose and muscle tissues (for example assessment of Akt/IRS1 phosphorylation by western blot analysis).

  through to study completion, an average of 14 months

Study Arms (2)

Young males aged 20-35 years old

Maintaining a normal daily living lifestyle * Healthy * Reasonably active- PAL: 1.4-1.9 * Non-obese- Fat mass index based on DEXA of 4-8kg/m2 * Weight stable for more than 3 months (±3% body mass) * Non-smoker * No chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, peripheral arterial disease, insulin- or non-insulin dependent diabetes or other metabolic disorders * No daily consumption of analgesic or anti-inflammatory drug(s) including NSAIDs and corticosteroids, prescription or non-prescription * No medications that may influence lipid or carbohydrate metabolism or immune system function * No known negative reaction to lidocaine * No participation in heavy resistance training

Other: Maintaining a normal daily living lifestyle

Old males aged 65-85 years old

Maintaining a normal daily living lifestyle * Healthy * Reasonably active- PAL: 1.4-1.9 * Non-obese- Fat mass index based on DEXA of 4-8kg/m2 * Weight stable for more than 3 months (±3% body mass) * Non-smoker * No chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, peripheral arterial disease, insulin- or non-insulin dependent diabetes or other metabolic disorders * No daily consumption of analgesic or anti-inflammatory drug(s) including NSAIDs and corticosteroids, prescription or non-prescription * No medications that may influence lipid or carbohydrate metabolism or immune system function * No known negative reaction to lidocaine * No participation in heavy resistance training

Other: Maintaining a normal daily living lifestyle

Interventions

Maintaining a normal daily living lifestyle OTHER

participants will undertake a normal lifestyle with no alterations to daily living in order to access the differences in tissue (muscle and adipose) and systemic inflammation between the two age cohorts

Old males aged 65-85 years old; Young males aged 20-35 years old

Eligibility Criteria

• Age: 20 Years - 85 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

general free-living public in the Bath and the surrounding areas

You may qualify if:

• Male
• Aged between 20-35 years (Group 1) or 65-85 years (Group 2)
• Fat mass index based on DEXA between 4-8 kg/m2
• Weight stable for more than 3 months (no change in weight +/- 3%)
• Physical Activity Level (PAL) between 1.4 and 1.9
• Non-smoker
• Healthy

You may not qualify if:

• Any chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, peripheral arterial disease, insulin- or non-insulin dependent diabetes or other metabolic disorders
• Individuals who consume on a daily basis any analgesic or anti-inflammatory drug(s) including NSAIDs and corticosteroids, prescription or non-prescription
• Taking any medications that may influence lipid or carbohydrate metabolism or immune system function
• Individuals with a known negative reaction to lidocaine anaesthetic
• Participation in heavy resistance training

Sponsors & Collaborators

• University of Bath: lead

Study Sites (1)

university of bath - Department for Health

Bath, Somerset, BA2 7AY, United Kingdom

Location

Related Publications (20)

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Biospecimen

Retention: SAMPLES WITH DNA

muscle and adipose biopsies blood plasma blood serum peripheral blood mononuclear cells

MeSH Terms

Conditions

Inflammation

Condition Hierarchy (Ancestors)

Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Officials

• Dylan Thompson, Professor

  University of Bath

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Ph.D. Student

Study Record Dates

• First Submitted: May 6, 2016
• First Posted: May 19, 2016
• Study Start: May 1, 2016
• Primary Completion: March 1, 2018
• Study Completion: October 1, 2018
• Last Updated: August 24, 2018

Record last verified: 2018-08

Data Sharing

• IPD Sharing: Will share

Locations

GB (1)