Anesthetic Effect on Immune Cell in Patients With Cancer

NCT02758249 | Completed

• 1 other identifier: KUH1160098
• Study Type: interventional
• Target: 47
• Locations: 1 country
• Sites: 1

Brief Summary

Anesthetics agents has an effect on immune response during the cancer surgery.This influence can regulatory to immune activity or cancer cell survival. The purpose of this study is to prove the variation of immune cell activity between preoperative and postoperative period.

Conditions

Breast Cancer

Outcome Measures

Primary Outcomes (1)

• immune cell (NK cell and CD8+ T cell)

  Patients blood sample are collect up to 24 hours postoperatively. These blood sample are collect in EDTA tube for NK cell and CD8+ T cell isolation from peripheral blood mononuclear cells (PBMCs). Isolation for NK cell, Ab stain with CD16 and CD56. Also, isolation for CD8+ T cell, Ab stain with CD8, that are purified from PBMCs, using FACSAria according to the manufacturer's protocol. These cell are culture with MCF-7 cancer cell line for 24 hours. After 24h, the degree of apoptosis of NK cell and CD8+ T cell was determined by flow cytometry. Suspension cell is NK cell or CD8+ T cell, these cells were harvest and washed with cell staining buffer. After washing, cell resuspended with Annexin-V binding buffer and stained with FITC-Annexin-V according to the manufacturer's protocol with analysis

  up to 24 hours

Secondary Outcomes (1)

• cancer cell (MCF-7) apoptosis

  Baseline. postoperative 1 hours and 24 hours

Study Arms (2)

sevoflurane

ACTIVE COMPARATOR

patients under sevoflurane anesthesia

Drug: Sevoflurane

propofol

ACTIVE COMPARATOR

patients under propofol anesthesia

Drug: propofol

Interventions

Sevoflurane DRUG

patients are anesthetized by using sevoflurane inhalation

sevoflurane

propofol DRUG

patients are anesthetized by using propofol infusion

propofol

Eligibility Criteria

• Age: 20 Years+
• Sex: female
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• patient who was planned to undergo breast cancer surgery.

You may not qualify if:

• age \< 20 years old
• history of hypersensitivity reaction in propofol or sevoflurane
• history of previous cancer
• patient with ongoing inflammation

Sponsors & Collaborators

• Konkuk University Medical Center: lead

Study Sites (1)

Konkuk University Medical Center

Seoul, 143-729, South Korea

Location

Related Publications (1)

• Lim JA, Oh CS, Yoon TG, Lee JY, Lee SH, Yoo YB, Yang JH, Kim SH. The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis. BMC Cancer. 2018 Feb 7;18(1):159. doi: 10.1186/s12885-018-4064-8.

  PMID: 29415668 DERIVED

MeSH Terms

Conditions

Breast Neoplasms

Interventions

Sevoflurane; Propofol

Condition Hierarchy (Ancestors)

Neoplasms by Site; Neoplasms; Breast Diseases; Skin Diseases; Skin and Connective Tissue Diseases

Intervention Hierarchy (Ancestors)

Methyl Ethers; Ethers; Organic Chemicals; Hydrocarbons, Fluorinated; Hydrocarbons, Halogenated; Hydrocarbons; Phenols; Benzene Derivatives; Hydrocarbons, Aromatic; Hydrocarbons, Cyclic

Study Officials

• Seong-Hyop Kim, M.D., Ph.D.

  Konkuk University Medical Center

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: FACTORIAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: February 26, 2016
• First Posted: May 2, 2016
• Study Start: January 1, 2016
• Primary Completion: January 1, 2016
• Study Completion: January 1, 2016
• Last Updated: January 25, 2018

Record last verified: 2018-01

Data Sharing

• IPD Sharing: Will not share

Locations

KR (1)