Morphometrics and Morphokinetics of Thawed Oocytes and Embryos

NCT02639715 | Completed

• 1 other identifier: S55685
• Study Type: observational
• Target: 1,272
• Locations: 0 countries
• Sites: N/A

Brief Summary

The aim of this observational study is to describe for the first time a complete set of morphometric and morphokinetic parameters of frozen-thawed human embryos in order to select the embryo with the highest implantation potential. These parameters will be correlated with (1) data of the fresh embryos and their subsequent survival, the evolution of the contact surfaces between surviving blastomeres and the resumption of mitosis; (2) patient characteristics and clinical outcome of frozen-thawed embryos and (3) a comparison between embryos cryopreserved with vitrification or the slow method regarding these parameters will be performed. This project aims to introduce new evaluation criteria of the frozen-thawed embryos to improve success rate of FET cycles. By establishing new precise, reliable and non-invasive measurable parameters, we aim to (1) select which supernumerary embryos are at chance to survive the freezing/thawing procedures, (2) establish cut off parameters for the survival rate of the embryo, (3) assess implantation, pregnancy and live birth rates depending on the developmental characteristics after thawing and after 24h of culture. Our strategy for improving the outcome of FET cycles is based on the study of morphometric and morphokinetic parameters in frozen-thawed embryos and the correlation with embryological, clinical and cycle parameters. Transferring a well-defined thawed embryo with a high implantation potential may lead towards an increase in the implantation and life birth rate and an increase in the cumulative live birth rate with a reduction of the IVF costs in order to optimize the health-economic situation of reproductive medicine.

Conditions

Infertility

Outcome Measures

Primary Outcomes (1)

• embryo implantation (measured by the number of intrauterine fetal sacs on ultrasound per number of embryos transferred) after a thawing / warming cycle

  6-7 weeks

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Patients with frozen thawed embryos for an ART treatment cycle

You may qualify if:

• patients with female age \< 40 years at Oocyte retrieval

You may not qualify if:

• Patients with female age \>40 years at Oocyte retrieval
• cycles with Preimplantation genetic diagnosis
• donated gametes

Sponsors & Collaborators

• Sophie Debrock: lead

Related Publications (2)

• Fernandez Gallardo E, Spiessens C, D'Hooghe T, Debrock S. Effect of day 3 embryo morphometrics and morphokinetics on survival and implantation after slow freezing-thawing and after vitrification-warming: a retrospective cohort study. Reprod Biol Endocrinol. 2017 Oct 3;15(1):79. doi: 10.1186/s12958-017-0299-5.

  PMID: 28974230 DERIVED

• Fernandez Gallardo E, Spiessens C, D'Hooghe T, Debrock S. Effect of embryo morphology and morphometrics on implantation of vitrified day 3 embryos after warming: a retrospective cohort study. Reprod Biol Endocrinol. 2016 Jul 30;14(1):40. doi: 10.1186/s12958-016-0175-8.

  PMID: 27475526 DERIVED

MeSH Terms

Conditions

Infertility

Condition Hierarchy (Ancestors)

Genital Diseases; Urogenital Diseases

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Professor Doctor PhD

Study Record Dates

• First Submitted: December 15, 2015
• First Posted: December 24, 2015
• Study Start: January 1, 2014
• Primary Completion: December 1, 2014
• Study Completion: December 1, 2015
• Last Updated: December 24, 2015

Record last verified: 2015-12