NCT02622802

Brief Summary

The most important guideline for drug prescription concerning pregnant women is 'drugs should be given only if the maternal benefits outweigh the potential risk to the fetus'. However, poor data is available on maternal drug disposition and transfer through the placenta, so the evidence available for decision making in clinical practice is weak. An ex-vivo placenta perfusion model will be used to explore the mechanisms governing differences between fetal and maternal drug exposure. The expression of placental transporters and cytochrome P450 (CYP) enzymes will be investigated in primary placenta cell culture and placental biopsies from different gestational stages to learn how the placental drug transfer and disposition is regulated. The investigators choose to examine the transfer of paracetamol, erythromycin and azithromycin because these drugs are commonly used in human pregnancies and have different metabolic pathways.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
250

participants targeted

Target at P50-P75 for not_applicable pregnancy

Timeline
Completed

Started Nov 2012

Longer than P75 for not_applicable pregnancy

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2012

Completed
2.6 years until next milestone

First Submitted

Initial submission to the registry

June 8, 2015

Completed
6 months until next milestone

First Posted

Study publicly available on registry

December 7, 2015

Completed
12 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2016

Completed
5 months until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2017

Completed
Last Updated

May 3, 2017

Status Verified

May 1, 2017

Enrollment Period

4.1 years

First QC Date

June 8, 2015

Last Update Submit

May 2, 2017

Conditions

Keywords

ex-vivo placenta perfusiontransplacental transferparacetamolerythromycinazithromycin

Outcome Measures

Primary Outcomes (1)

  • Transplacental transfer rate of drugs and their metabolites measured by comparing the concentrations in simultaneously collected samples from the maternal and fetal compartment in a human ex-vivo placenta perfusion model

    the drug concentrations will be determined by high performance liquid chromatography and mass spectrometry

    12 months for each drug

Study Arms (1)

ex-vivo placenta perfusion

EXPERIMENTAL

ex vivo placenta perfusion study with exposure to paracetamol, erythromycin and azithromycin

Drug: paracetamolDrug: ErythromycinDrug: Azithromycin

Interventions

In an ex vivo placenta perfusion study, placental tissue is exposed to paracetamol

Also known as: Paracetamol Sigma Aldrich, acetaminophen
ex-vivo placenta perfusion

In an ex vivo placenta perfusion study, placental tissue is exposed to erythromycin

Also known as: Erythromycin Sigma Aldrich
ex-vivo placenta perfusion

In an ex vivo placenta perfusion study, placental tissue is exposed to azithromycin

Also known as: Azithromycin Sigma Aldrich
ex-vivo placenta perfusion

Eligibility Criteria

Age18 Years - 45 Years
Sexfemale
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • informed consent has been signed
  • (placenta of a) pregnant women with an uncomplicated pregnancy and delivery

You may not qualify if:

  • use of medication during pregnancy
  • hypertension, diabetes
  • smoking

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University Hospital Gasthuisberg

Leuven, 3000, Belgium

Location

MeSH Terms

Interventions

AcetaminophenErythromycinAzithromycin

Intervention Hierarchy (Ancestors)

AcetanilidesAnilidesAmidesOrganic ChemicalsAniline CompoundsAminesMacrolidesPolyketidesLactones

Study Officials

  • Kristel Van Calsteren, MD PhD

    University Hospital Gasthuisberg Leuven

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
BASIC SCIENCE
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Prof Dr

Study Record Dates

First Submitted

June 8, 2015

First Posted

December 7, 2015

Study Start

November 1, 2012

Primary Completion

December 1, 2016

Study Completion

May 1, 2017

Last Updated

May 3, 2017

Record last verified: 2017-05

Locations