NCT02601053

Brief Summary

For centuries the term "blood curling" has been used to describe extreme frightening situations. However, the origin of this ancient theory has never been studied and it remains unknown if fear induces the coagulation system.The objective was to explore the effects of acute fear on the coagulation system while sitting still. In a crossover study design healthy subjects will be exposed to a horrifying e.g. scary movie followed by a dull e.g. flat movie which is shown at least 1 week after the first movie on the same day of the week at the same time of the day. Participants will be recruited among students from the Leiden University Medical Center. Blood will be drawn from the cubital vein 10 minutes before the first movie, directly after the first movie. The same will be done 10 minutes before and directly after the second movie. Blood is drawn by using a needle puncture. Individual markers of coagulation activity will be determined from the blood samples. Pulse rates will be measured and an anxiety/fear score will be collected from each student for both movies.

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
24

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Jun 2015

Shorter than P25 for not_applicable

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 1, 2015

Completed
2 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

August 1, 2015

Completed
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

September 1, 2015

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

November 5, 2015

Completed
5 days until next milestone

First Posted

Study publicly available on registry

November 10, 2015

Completed
Last Updated

March 5, 2025

Status Verified

March 1, 2025

Enrollment Period

2 months

First QC Date

November 5, 2015

Last Update Submit

March 4, 2025

Conditions

Outcome Measures

Primary Outcomes (4)

  • Change in Factor VIII:C (mean change before and after movie) between movies

    The mean change in FVIII:C (in IU/dL) levels after seeing a scary movie (ie from timepoint 0 to 1 in study arm I and from timepoint 2 to timepoint 3 for treatment arm II will be compared to the mean change after seeing a dull movie. A paired student t-test is used to compare the two mean changes in coagulation markers.

    Four time points (TP); (0) before the first movie (baseline), after the first movie [90 minutes](1), before the second movie (baseline, 2) and after the second movie [90 minutes](3). All blood will be drawn within 15 minutes before or after the movie.

  • Change in D-dimer (mean change before and after movie) between movies

    The mean change in D-dimer(in ng/mL) levels after seeing a scary movie (ie from timepoint 0 to 1 in study arm I and from timepoint 2 to timepoint 3 for treatment arm II will be compared to the mean change after seeing a dull movie. A paired student t-test is used to compare the two mean changes in coagulation markers.

    Four time points (TP); (0) before the first movie (baseline), after the first movie [90 minutes](1), before the second movie (baseline, 2) and after the second movie [90 minutes](3). All blood will be drawn within 15 minutes before or after the movie.

  • Change in Thrombin and Antithrombin complexes (TATc) (mean change before and after movie) between movies

    The mean change in TATc (in mcg/L) levels after seeing a scary movie (ie from timepoint 0 to 1 in study arm I and from timepoint 2 to timepoint 3 for treatment arm II will be compared to the mean change after seeing a dull movie. A paired student t-test is used to compare the two mean changes in coagulation markers.

    Four time points (TP); (0) before the first movie (baseline), after the first movie [90 minutes](1), before the second movie (baseline, 2) and after the second movie [90 minutes](3). All blood will be drawn within 15 minutes before or after the movie.

  • Change in Prothrombin fragments 1+2 (F1+2) (mean change before and after movie) between movies

    The mean change in F1+2 (in umol/dL) levels after seeing a scary movie (ie from timepoint 0 to 1 in study arm I and from timepoint 2 to timepoint 3 for treatment arm II will be compared to the mean change after seeing a dull movie. A paired student t-test is used to compare the two mean changes in coagulation markers.

    Four time points (TP); (0) before the first movie (baseline), after the first movie [90 minutes](1), before the second movie (baseline, 2) and after the second movie [90 minutes](3). All blood will be drawn within 15 minutes before or after the movie.

Secondary Outcomes (2)

  • Difference of Heart Rate (peaks in heart rate) between movies

    During the entire movie the heart rate is recorded [up to 90 minutes]. Heart rate peaks (above average) will be compared between movies in one individual.

  • Difference of the Visual Analogue Scale on Fear between movies

    VAS scale, directly after dull movie [90 minutes] and directly after scary movie [90 minutes] for each individual

Study Arms (2)

Dull (boring) movie

ACTIVE COMPARATOR

Participants will be exposed to a dull (i.e. boring) movie followed by a scary (i.e. horrifying) movie.

Other: Dull movieOther: Scary (horrifying) movieOther: Heart rate measurement

Scary (horrifying) movie

EXPERIMENTAL

Participants will be exposed to a scary (i.e. horrifying) movie followed by a to a dull (i.e. boring) movie.

Other: Dull movieOther: Scary (horrifying) movieOther: Heart rate measurement

Interventions

A dull movie (duration approximately 90 minutes)

Dull (boring) movieScary (horrifying) movie

A scary movie (duration approximately 90 minutes)

Dull (boring) movieScary (horrifying) movie

Heart rate measurement using a actiheart device

Dull (boring) movieScary (horrifying) movie

Eligibility Criteria

Age18 Years - 30 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy volunteers between 18 and 30 years old
  • No medication and otherwise healthy

You may not qualify if:

  • Any coagulation disorder
  • Hormonal anticonception (excluding intrauterine devices)
  • Pregnancy or puerperium
  • History of Venous thrombosis
  • Major surgery or cast immobilisation in the past two months
  • Neoplasm or inflammatory disease

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Publications (1)

  • Nemeth B, Scheres LJ, Lijfering WM, Rosendaal FR. Bloodcurdling movies and measures of coagulation: Fear Factor crossover trial. BMJ. 2015 Dec 16;351:h6367. doi: 10.1136/bmj.h6367.

MeSH Terms

Conditions

Blood Coagulation Disorders

Interventions

Motion PicturesHeart Rate

Condition Hierarchy (Ancestors)

Hematologic DiseasesHemic and Lymphatic Diseases

Intervention Hierarchy (Ancestors)

Audiovisual AidsEducational TechnologyTechnologyTechnology, Industry, and AgricultureVital SignsPhysical ExaminationDiagnostic Techniques and ProceduresDiagnosisHemodynamicsCardiovascular Physiological PhenomenaCirculatory and Respiratory Physiological Phenomena

Study Officials

  • Banne Nemeth, MD

    Leiden University Medical Center

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
OTHER
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
MD

Study Record Dates

First Submitted

November 5, 2015

First Posted

November 10, 2015

Study Start

June 1, 2015

Primary Completion

August 1, 2015

Study Completion

September 1, 2015

Last Updated

March 5, 2025

Record last verified: 2025-03