Bioavailability and Absorption Kinetics of Phenolic Compounds and Specific Vitamins

NCT02587468 | Completed

• 1 other identifier: 27-507 ex 14/15
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 2

Brief Summary

GOAL To identify absorption kinetics and bioavailability of phenolic compounds and specific vitamins from encapsulated Juice Plus+® powder ENDPOINTS

• Absorption kinetics of phenolic compounds in plasma
• Bioavailability of vitamins C, E, and carotenoids into plasma Subjects: 20 healthy subjects from Graz region, meeting all inclusion criterions (see underneath).

Conditions

Healthy

Keywords

phenolics; absorption; fruit & vegetable concentrate; Bioavailability of phenolic compounds

Outcome Measures

Primary Outcomes (1)

• Absorption kinetics of phenolic acids after 1, 2, 5 and 10 hours, measured via HPLC, evaluated by repeated measures ANOVA, and assessed via SPSS 19.0.

  For the phenolics analyses subjects come to the lab after an overnight fast, and after 2 weeks wash-out of all food supplements and 48 hours wash-out of phytonutrients (phytonutrient-poor diet). To adhere to the phytonutrient-poor diet they receive an exact diet plan in the forefront. This plan will be perused with the research associate. After the baseline blood drawing, they receive 3 capsules of Juice Plus+® consumed with 250ml plain water from the pipe. They will be instructed to swallow one capsule of each blend (fruit, berry, vegetable). During this day 4 additional blood samples will be drawn: 1hr, 2hrs, 5hrs, 10hrs after capsule intake in the morning. After the 2hrs blood sampling the subjects get a standardized, phytonutrient-poor snack: white bread, cheese, ham, milk and water ad libitum. Subjects will receive a list with suggestions of allowed food they could consume on this day.

  1 year

Secondary Outcomes (1)

• Absorption of ß-carotene after 8 weeks of supplementation, measured via HPLC, evaluated by paired t-test, and assessed via SPSS 19.0

  1 year

Study Arms (1)

Juice Plus+(R)

EXPERIMENTAL

Check of phenolic absorption between baseline and after 8wks of intake before-after comparison

Dietary Supplement: Juice Plus+(R)

Interventions

Juice Plus+(R) DIETARY_SUPPLEMENT

before-after comparison of plasma phenolics

Juice Plus+(R)

Eligibility Criteria

• Age: 20 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy men and women, 20-50 years old;
• non-smokers;
• BMI \>20 und \<30 kg/m2;
• no medication;
• premenopausal;
• normal dietary habits (no specific diets, meals, food components etc.);
• adherence to wash-out period.

You may not qualify if:

• diet: subjects consuming \>4 servings of fruits and vegetables per day;
• subjects with any kind of food allergy or histamine intolerance;
• highly trained subjects (\> 5 training units/week);
• women with menstrual dysfunctions;
• pregnancy
• alcoholics.

Sponsors & Collaborators

• Green Beat: lead
• Medical University of Graz: collaborator

Study Sites (2)

Institute of Pathophysiology and Immunology

Graz, Styria, 8010, Austria

Location

Green Beat

Graz, 8042, Austria

Location

Study Officials

• Manfred Lamprecht, PhD

  Green Beat + Medical University of Graz

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: October 22, 2015
• First Posted: October 27, 2015
• Study Start: October 1, 2015
• Primary Completion: June 1, 2017
• Study Completion: July 1, 2017
• Last Updated: August 1, 2017

Record last verified: 2017-07

Locations

AU (2)