Exercise Induced Changes in Fat Metabolism

NCT02581306 | Completed

• 1 other identifier: HUM00103937
• Study Type: interventional
• Target: 29
• Locations: 1 country
• Sites: 1

Brief Summary

If eligible to participate in the research study after completing the physical screening procedures, participants will be asked to return to the Substrate Metabolism Laboratory on another occasion after a short fast (no food or beverages - besides water) for at least 6 hours. After participants arrive, participants will rest quietly for approximately 30 min. The investigators will then collect a blood sample from a vein in participants hand, or forearm. After obtaining a baseline blood sample, participants will exercise at a moderate intensity (on a treadmill or a stationary bike) for one hour. This one hour moderate exercise session will feel like a slow jog during which participants would be able to carry a conversation. In order for the investigators to measure energy expended and the amount of fat participants are burning during exercise, the investigators will periodically ask participants to breathe through a mouthpiece so the investigators can collect participants expired breath. After the exercise session the investigators will collect another blood sample from a vein in participants hand, or forearm.

Conditions

Healthy Volunteers

Outcome Measures

Primary Outcomes (6)

• changes in plasma concentrations of epinephrine after a session of exercise

  Plasma levels will be measured using appropriate commercially available assays.

  1 day

• changes in plasma concentrations of lactate after a session of exercise

  Plasma levels will be measured using appropriate commercially available assays.

  1 day

• changes in plasma concentrations of fatty acids after a session of exercise

  Plasma levels will be measured using appropriate commercially available assays.

  1 day

• changes in plasma concentrations of IL-6 after a session of exercise

  Plasma levels will be measured using appropriate commercially available assays.

  1 day

• changes in plasma concentrations of irisin after a session of exercise

  Plasma levels will be measured using appropriate commercially available assays.

  1 day

• changes in plasma concentrations of growth hormone after a session of exercise

  Plasma levels will be measured using appropriate commercially available assays.

  1 day

Study Arms (1)

Exercise session

OTHER

All subjects will exercise for 1 hour at a moderate intensity. There are no different arms in this study

Other: Exercise session

Interventions

Exercise session OTHER

There is no drug or device intervention in this study. Subjects will exercise at a moderate intensity for 60 minutes and a blood sample will be obtained before and after the exercise session

Exercise session

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age: 18-40
• Body Mass Index: 20-35 kg/m2
• Women must have regularly occurring menses and must be premenopausal

You may not qualify if:

• Pregnant or lactating
• Evidence/history of cardiovascular or metabolic disease
• Medications known to affect lipid or glucose metabolism

Sponsors & Collaborators

• University of Michigan: lead

Study Sites (1)

University of Michigan

Ann Arbor, Michigan, 48109, United States

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor, Movement Science and Director, Substrate Metabolism Laboratory

Study Record Dates

• First Submitted: October 13, 2015
• First Posted: October 20, 2015
• Study Start: October 1, 2015
• Primary Completion: May 11, 2016
• Study Completion: May 11, 2016
• Last Updated: March 21, 2022

Record last verified: 2022-03

Locations

US (1)