NCT02579967

Brief Summary

Background: Allogeneic blood or marrow transplant is when stem cells are taken from one person s blood or bone marrow and given to another person. Researchers think this may help people with immune system problems. Objective: To see if allogeneic blood or bone marrow transplant is safe and effective in treating people with primary immunodeficiencies. Eligibility: Donors: Healthy people ages 4 or older Recipients: People ages 4-75 with a primary immunodeficiency that may be treated with allogeneic blood or marrow transplant Design: Participants will be screened with medical history, physical exam, and blood tests. Participants will have urine tests, EKG, and chest x-ray. Donors will have: Bone marrow harvest: With anesthesia, marrow is taken by a needle in the hipbone. OR Blood collection: They will have several drug injections over 5-7 days. Blood is taken by IV in one arm, circulates through a machine to remove stem cells, and returned by IV in the other arm. Possible vein assessment or pre-anesthesia evaluation Recipients will have: Lung test, heart tests, radiology scans, CT scans, and dental exam Possible tissue biopsies or lumbar puncture Bone marrow and a small piece of bone removed by needle in the hipbone. Chemotherapy 1-2 weeks before transplant day Donor stem cell donation through a catheter put into a vein in the chest or neck Several-week hospital stay. They will take medications and may need blood transfusions and additional procedures. After discharge, recipients will: Remain near the clinic for about 3 months. They will have weekly visits and may require hospital readmission. Have multiple follow-up visits to the clinic in the first 6 months, and less frequently for at least 5 years.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
354

participants targeted

Target at P75+ for phase_2

Timeline
130mo left

Started Nov 2015

Longer than P75 for phase_2

Geographic Reach
1 country

2 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress50%
Nov 2015Dec 2036

First Submitted

Initial submission to the registry

October 16, 2015

Completed
4 days until next milestone

First Posted

Study publicly available on registry

October 20, 2015

Completed
1 month until next milestone

Study Start

First participant enrolled

November 19, 2015

Completed
16.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2031

Expected
5 years until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2036

Last Updated

April 29, 2026

Status Verified

April 22, 2026

Enrollment Period

16.1 years

First QC Date

October 16, 2015

Last Update Submit

April 28, 2026

Conditions

Keywords

HaploidenticalAutoimmunityImmune DysregulationCongenitalOpportunistic Infection

Outcome Measures

Primary Outcomes (3)

  • For the RIC-SHORT arm: To estimate the aGVHD-free, graft failure-free survival

    Proportion of participants without GVHD

    +180 after allo BMT

  • For the RIC : To estimate the aGVHD-free, graft failure-free survival

    Proportion of participants without GVHD

    +180 after allo BMT

  • For the RIC-MMF arm: To determine the shortest duration of MMF that can be safely administered without excessive rates of graft failure or acute grade 3-4 GVHD

    Shortest duration of MMF

    Duration de-escalation design

Secondary Outcomes (9)

  • Transplant-related mortality

    +180 and 1 year post transplant

  • Secondary graft failure

    1 year post transplant

  • Overall survival

    1 year post transplant

  • Kinetics and durability of lineage-specific donor chimerism

    days +28 and +42

  • Kinetics and durability of engraftment

    days +28, +42, +60, +100, +180, and 1 year after allo BMT

  • +4 more secondary outcomes

Study Arms (6)

1/ IOC Arm-Closed with amendment L (07/05/2019)

EXPERIMENTAL

Immunosuppression Only Conditioning Arm

Drug: Immunosuppression Only Conditioning -Closed with amendment LDrug: GVHD ProphylaxisProcedure: Allo BMT

2/ RIC Arm - Closed with Amendment L (07/05/2019)

EXPERIMENTAL

Reduced Intensity Conditioning Arm

Drug: Reduced Intensity ConditioningDrug: GVHD ProphylaxisProcedure: Allo BMT

3/ MAC Arm-Closed with amendment L (07/05/2019)

EXPERIMENTAL

Myeloablative Conditioning Arm

Drug: Myeloablative Conditioning-Closed with amendment LDrug: GVHD ProphylaxisProcedure: Allo BMT

4/ RIC-MMF Arm

EXPERIMENTAL

Reduced Intensity Conditioning with MMF duration de-escalation design

Drug: Reduced Intensity ConditioningDrug: GVHD ProphylaxisProcedure: Allo BMT

5/ Donor Arm

NO INTERVENTION

Donor

6/ RIC-SHORT Arm

EXPERIMENTAL

Reduced Intensity Conditioning with shortened duration and dose-reduced PTCy

Drug: Reduced Intensity ConditioningDrug: GVHD ProphylaxisProcedure: Allo BMT

Interventions

pentostatin 4 mg/m2/day IV on days -11 and -7, cyclophosphamide 3 mg/kg orally daily on days -11 through -4; busulfan IV, pharmokinetically dosed, on days -3 and -2.

2/ RIC Arm - Closed with Amendment L (07/05/2019)4/ RIC-MMF Arm6/ RIC-SHORT Arm

Pentostatin 4 mg/m2/day IV on days -13 and -9, low-dose cyclophosphamide orally daily on days -13 through -6; busulfan IV, pharmokinetically dosed, on days -5, -4, -3, and -2. (Closed with amendment L)

3/ MAC Arm-Closed with amendment L (07/05/2019)

Pentostatin 4 mg/m2/day IV on days -9 and -5, cyclophosphamide 5 mg/kg orally daily on days -9 through -2 (Closed with amendment L)

1/ IOC Arm-Closed with amendment L (07/05/2019)

High-dose, post-transplantation cyclophosphamide (PTCy) 25-50 mg/kg on days +3 and +4, sirolimus 6 mg on days +5 through +90, and mycophenolate mofetil (MMF) on days +5 through 0, +18, +25, or +35 depending on treatment arm and cohort.

1/ IOC Arm-Closed with amendment L (07/05/2019)2/ RIC Arm - Closed with Amendment L (07/05/2019)3/ MAC Arm-Closed with amendment L (07/05/2019)4/ RIC-MMF Arm6/ RIC-SHORT Arm
Allo BMTPROCEDURE

Allogeneic blood or marrow transplantation

1/ IOC Arm-Closed with amendment L (07/05/2019)2/ RIC Arm - Closed with Amendment L (07/05/2019)3/ MAC Arm-Closed with amendment L (07/05/2019)4/ RIC-MMF Arm6/ RIC-SHORT Arm

Eligibility Criteria

Age4 Years - 75 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Patients age \>= 4 through 75 years
  • PID deemed to be of sufficient past severity to warrant allo BMT, by meeting the two criteria below:
  • PID as defined by identified genetic defect or, in the absence of a PID-associated genetic mutation, patients with an immune defect potentially amenable to allo BMT who meet the clinical history criteria below may be eligible upon discussion with the PI
  • Mutations should be confirmed in a CLIA-certified laboratory, if such testing is available.
  • Patients without a mutation must be deemed eligible and appropriate for allo BMT by the PI. Some patients may meet the clinical history criteria listed below, but will not be eligible if it is thought that their clinical history is due to a condition apart from an immune defect. In addition, patients with a PID of mild severity, such as those with selective IgA deficiency, may meet at least two of the clinical history criteria, but may be deemed inappropriate for allo BMT by the PI if it is felt that the risks of the procedure outweigh the severity of the disease.
  • Clinical history of at least two of the following:
  • Life-threatening, organ-threatening, or severely disfiguring infection
  • Protracted or recurrent infections requiring unusually long or repeated courses of antibiotics
  • Infection with an opportunistic organism
  • Chronic elevation in the blood (\>=2 documented elevations over a period of 6 months or longer) of a latent virus (EBV, CMV, HHV6, HHV8, etc.)
  • Evidence of immune dysregulation, as manifested by autoimmune disease, atopy, hemophagocytic lymphohistiocytosis/macrophage activation syndrome, granulomas, splenomegaly, or lymphadenopathy
  • Patients with hemophagocytic lymphohistiocytosis or macrophage activation syndrome related to an underlying lymphoma with no other clinical history suggestive of a primary immunodeficiency will not be eligible
  • Hypogammaglobulinemia, dysglobulinemia, or impaired response to vaccination
  • Hematologic malignancy or lymphoproliferative disorder
  • Tissue diagnosis should be confirmed by NCI Department of Pathology, if prior biopsies are available
  • +13 more criteria

You may not qualify if:

  • Patients who are receiving any other investigational agents, with the exception of virus-specific cytotoxic T-cells for the treatment of viral infection/reactivation prior to allo BMT.
  • History of allergic reactions attributed to compounds of similar chemical or biologic composition to agents (cyclophosphamide, busulfan, pentostatin, sirolimus, MMF, filgrastim or filgrastim biosimilar) used in the study
  • Active psychiatric disorder which may compromise compliance with the transplant protocol, or which does not allow for appropriate informed consent
  • Active central nervous system (CNS) involvement by malignancy, except in cases of virus-associated malignancies with CNS involvement in which case the patient may benefit from the transplant to control the malignancy.
  • MAGT1 mutation and active need to take anti-platelet agents and/or therapeutic anti-coagulation that cannot be interrupted during aplasia.
  • HIV positive or other acquired immunodeficiency that, as determined by the PI, interferes with the assessment of PID severity and/or the attribution of clinical manifestations of immunodeficiency to a PID.
  • Lack of adequate central venous access potential
  • Ages \>= 4
  • Related donor deemed suitable and eligible and willing to donate per clinical evalations who are additionally willing to donate blood, urine, and marrow specimens for research. Related donors will be evaluated in accordance with existing Standard Policies and Procedures for determination of eligibility and suitability for clinical donation. Note that participation in this study is offered to all related donors but is not required for clinical donation, so it is possible that not all related donors will enroll on this study.
  • None
  • Ages \>= 18
  • Unrelated donors will be evaluated in accordance with existing NMDP Standard Policies and Procedures, available at: http://bethematch.org/About-Us/Global-transplant-network/Standards/, except for the additional requirement of EBV serostatus testing. Note that participation in this study is offered to all unrelated donors but not required for clinical donation, so it is possible that not all unrelated donors will enroll on this study.
  • Unrelated donors: failure to qualify as a National Marrow Donor Program (NMDP) donor per current NMDP Standards, available at: http://bethematch.org/About-Us/Global-transplant-network/Standards/.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

National Institutes of Health Clinical Center

Bethesda, Maryland, 20892, United States

RECRUITING

National Marrow Donor Program

Minneapolis, Minnesota, 55413-1753, United States

RECRUITING

Related Publications (3)

  • Bashey A, Zhang X, Sizemore CA, Manion K, Brown S, Holland HK, Morris LE, Solomon SR. T-cell-replete HLA-haploidentical hematopoietic transplantation for hematologic malignancies using post-transplantation cyclophosphamide results in outcomes equivalent to those of contemporaneous HLA-matched related and unrelated donor transplantation. J Clin Oncol. 2013 Apr 1;31(10):1310-6. doi: 10.1200/JCO.2012.44.3523. Epub 2013 Feb 19.

    PMID: 23423745BACKGROUND
  • Mariotti J, Taylor J, Massey PR, Ryan K, Foley J, Buxhoeveden N, Felizardo TC, Amarnath S, Mossoba ME, Fowler DH. The pentostatin plus cyclophosphamide nonmyeloablative regimen induces durable host T cell functional deficits and prevents murine marrow allograft rejection. Biol Blood Marrow Transplant. 2011 May;17(5):620-31. doi: 10.1016/j.bbmt.2010.11.029. Epub 2010 Dec 3.

    PMID: 21130889BACKGROUND
  • Kang E, Gennery A. Hematopoietic stem cell transplantation for primary immunodeficiencies. Hematol Oncol Clin North Am. 2014 Dec;28(6):1157-70. doi: 10.1016/j.hoc.2014.08.006. Epub 2014 Sep 16.

    PMID: 25459185BACKGROUND

Related Links

MeSH Terms

Conditions

T cell immunodeficiency primaryCommon Variable ImmunodeficiencyImmune System DiseasesLymphoproliferative DisordersAutoimmune DiseasesOpportunistic Infections

Condition Hierarchy (Ancestors)

Immunologic Deficiency SyndromesLymphatic DiseasesHemic and Lymphatic DiseasesImmunoproliferative DisordersInfections

Study Officials

  • Dimana Dimitrova, M.D.

    National Cancer Institute (NCI)

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Dimana Dimitrova, M.D.

CONTACT

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

October 16, 2015

First Posted

October 20, 2015

Study Start

November 19, 2015

Primary Completion (Estimated)

December 31, 2031

Study Completion (Estimated)

December 31, 2036

Last Updated

April 29, 2026

Record last verified: 2026-04-22

Data Sharing

IPD Sharing
Will share

All IPD recorded in the medical record will be shared with intramural investigators upon request. In addition, all large scale genomic sequencing data will be shared with subscribers to dbGAP

Shared Documents
STUDY PROTOCOL, SAP, ICF
Time Frame
Clinical data available during the study and indefinitely. Genomic data are available once genomic data are uploaded per protocol GDS plan for as long as the database is active.
Access Criteria
Clinical data will be made available and with permission of the study PI. Genomic data are made available via dbGAP through requests to the data custodians

Locations