Proteomics for Identification of Hyperoxia-induced Changes in Protein Expression
1 other identifier
interventional
40
0 countries
N/A
Brief Summary
Aim of the present study is to investigate the influence of hyperoxia on the protein expression using the differential analysis of protein expression in tissues (proteomics). In the study, blood and urine samples will be collected from participants who undergo a short term hyperoxia using 100% oxgen for 3 hours. Here, gel electrophoresis, protein separation and mass spectroscopy allow to identify affected proteins. Based on these results, different induction factors of proteins will be determined and then assessed using a bioinformatic network analysis regarding the cellular influence.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at P25-P50 for not_applicable
Started Oct 2015
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
Click on a node to explore related trials.
Study Timeline
Key milestones and dates
First Submitted
Initial submission to the registry
July 9, 2015
CompletedFirst Posted
Study publicly available on registry
September 17, 2015
CompletedStudy Start
First participant enrolled
October 1, 2015
CompletedPrimary Completion
Last participant's last visit for primary outcome
October 1, 2016
CompletedStudy Completion
Last participant's last visit for all outcomes
December 1, 2016
CompletedSeptember 17, 2015
September 1, 2015
1 year
July 9, 2015
September 16, 2015
Conditions
Keywords
Outcome Measures
Primary Outcomes (1)
Comparison of proteomics changes before and after short-term hyperoxia
4 weeks
Secondary Outcomes (8)
Spirometry Results: Forced vital capacity (FVC) [L]
2 days
Spirometry Results: Forced expiratory volume at one second (FEV1) [L]
2 days
Spirometry Results: Forced expiratory flow (FEF25-75) [L/s]
2 days
Spirometry Results: Peak expiratory flow (PEF) [L/s]
2 days
Vital parameter: Respiratory rate (RR) [1/min]
3 hours
- +3 more secondary outcomes
Study Arms (1)
Hyperoxia
EXPERIMENTALParticipants receive 100% oxygen
Interventions
Eligibility Criteria
You may qualify if:
- American Society of Anesthesiologists (ASA) 1
- \>18 years
- \< 50 years
You may not qualify if:
- American Society of Anesthesiologists (ASA) \> 1
- pregnant
- \<18 years
- \> 50 years
- frequent or recent drug intake
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Related Publications (2)
Spelten O, Wetsch WA, Wrettos G, Kalenka A, Hinkelbein J. Response of rat lung tissue to short-term hyperoxia: a proteomic approach. Mol Cell Biochem. 2013 Nov;383(1-2):231-42. doi: 10.1007/s11010-013-1771-y. Epub 2013 Aug 11.
PMID: 23934118BACKGROUNDHinkelbein J, Feldmann RE Jr, Kalenka A. Time-dependent alterations of cerebral proteins following short-term normobaric hyperoxia. Mol Cell Biochem. 2010 Jun;339(1-2):9-21. doi: 10.1007/s11010-009-0365-1. Epub 2010 Jan 5.
PMID: 20049628BACKGROUND
MeSH Terms
Conditions
Interventions
Condition Hierarchy (Ancestors)
Intervention Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Stefan Braunecker, M.D.
Universityhospital of Cologne
Central Study Contacts
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- NA
- Masking
- NONE
- Purpose
- BASIC SCIENCE
- Intervention Model
- SINGLE GROUP
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Anesthesiologist
Study Record Dates
First Submitted
July 9, 2015
First Posted
September 17, 2015
Study Start
October 1, 2015
Primary Completion
October 1, 2016
Study Completion
December 1, 2016
Last Updated
September 17, 2015
Record last verified: 2015-09