NCT02505789

Brief Summary

In many countries, numerous steps are taken to minimize the risk of infection from transfused blood products. Typically, blood banking organisations will screen for an array of infectious pathogens as part of their quality control protocol. While transmission of these tested agents via transfusion has become exceedingly rare, the risk of transfusion-transmitted infections for which testing is not currently performed continues to be a concern. Among these untested infectious agents is Epstein-Barr virus (EBV, also known as human herpesvirus-4). Most notably, infection with this virus in transplant recipients can give rise to a malignant disorder called post-transplant lymphoproliferative disease (PTLD), a life-threatening complication which is due to the uncontrolled expansion of EBV-infected cells. It is also associated with other complications such as hepatitis, hemophagocytic syndrome, etc. in transplant population. It is recognised that EBV infection can occurred in transfused immune suppressed graft recipients but the origin of the viral infection is still a matter of debate. It is a known fact that the EBV already present in the recipient's blood can undergo reactivation due to immune suppression. However, because it is known to occur more frequently in patients who are EBV-seronegative at the time of transplant, it is also accepted that primary infection contracted via an infected graft can be a source of virus. The question we are seeking to answer is whether immune suppressed graft recipients can acquire primary EBV infection via transfusion of blood products. EBV is present in the blood of most adults and cases of EBV transfusion-related infection have been reported. Transplant populations are generally transfused with very large volumes of blood products and our recent pilot study supports the possibility that transfusion-related EBV infection can be transmitted to pediatric hematopoietic stem cell (HSCT) recipients (Trottier et al, 2012). The aim of this study is to analyse the risk of EBV transmission through blood product transfusion in pediatric allogeneic HSCT patients.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
324

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started May 2013

Longer than P75 for all trials

Geographic Reach
1 country

3 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

May 1, 2013

Completed
2.2 years until next milestone

First Submitted

Initial submission to the registry

July 15, 2015

Completed
7 days until next milestone

First Posted

Study publicly available on registry

July 22, 2015

Completed
2.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2017

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

November 1, 2018

Completed
Last Updated

July 22, 2015

Status Verified

July 1, 2015

Enrollment Period

4.5 years

First QC Date

July 15, 2015

Last Update Submit

July 20, 2015

Conditions

Keywords

Epstein-Barr VirusPost-transplant lymphoproliferative disease (PTLD)InfectionCell TransplantsHerpesvirus 4, Human

Outcome Measures

Primary Outcomes (1)

  • Measure of the risk of EBV infection in HSCT pediatric recipients from blood products transfusions (red blood cells, platelets, plasma) by EBV PCR and serology testing

    EBV PCR and serology testing every 1-2 weeks until hospital discharge and at follow-up visit thereafter.

    1 month before transplantation to 1 year post-transplantation

Secondary Outcomes (3)

  • Incidence of post-transplant EBV infection in allogeneic HSCT pediatric recipients stratified according to the EBV serostatus of the patient and the EBV status of the graft

    1 month before transplantation to 1 year post-transplantation

  • Incidence of "high or increasing viral load EBV infection and PTLD" in allogeneic HSCT pediatric recipients stratified according to the EBV serostatus of the patient and the EBV status of the graft

    1 month before transplantation to 1 year post-transplantation

  • Description of other complications related to EBV infection in this transplant population

    1 month before transplantation to 1 year post-transplantation

Interventions

Blood units administered to patients with severe EBV infection will be traced back to the donors who in turn (with consent) will be serologically assessed for EBV, and all seropositive donors will have their EBV strain genotyped for comparison to the patient's strain.

Eligibility Criteria

AgeUp to 21 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64)
Sampling MethodNon-Probability Sample
Study Population

Only allogeneic HSCTs will be considered (no autologous grafts) because allogeneic grafts require a higher degree of immune suppression, which is known to increase risk with regard to EBV infection, development of PTLD and other EBV complications

You may qualify if:

  • patients receiving allogeneic HSCT (marrow, cord-blood, and peripheral blood stem cells)
  • age below 21 years
  • first HSCT

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

BC Children's Hospital

Vancouver, British Columbia, V6H 3N1, Canada

RECRUITING

Cancer Care Manitoba

Winnipeg, Manitoba, R3E 0V9, Canada

RECRUITING

St. Justine's Hospital

Montreal, Quebec, H3T 1C5, Canada

RECRUITING

Related Publications (2)

  • Trottier H, Buteau C, Robitaille N, Duval M, Tucci M, Lacroix J, Alfieri C. Transfusion-related Epstein-Barr virus infection among stem cell transplant recipients: a retrospective cohort study in children. Transfusion. 2012 Dec;52(12):2653-63. doi: 10.1111/j.1537-2995.2012.03611.x. Epub 2012 Mar 15.

    PMID: 22420319BACKGROUND
  • Alfieri C, Tanner J, Carpentier L, Perpete C, Savoie A, Paradis K, Delage G, Joncas J. Epstein-Barr virus transmission from a blood donor to an organ transplant recipient with recovery of the same virus strain from the recipient's blood and oropharynx. Blood. 1996 Jan 15;87(2):812-7.

    PMID: 8555507BACKGROUND

MeSH Terms

Conditions

Lymphoproliferative DisordersEpstein-Barr Virus InfectionsInfections

Condition Hierarchy (Ancestors)

Lymphatic DiseasesHemic and Lymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesHerpesviridae InfectionsDNA Virus InfectionsVirus DiseasesTumor Virus Infections

Study Officials

  • Helen Trottier, PhD

    St. Justine's Hospital

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Professor

Study Record Dates

First Submitted

July 15, 2015

First Posted

July 22, 2015

Study Start

May 1, 2013

Primary Completion

November 1, 2017

Study Completion

November 1, 2018

Last Updated

July 22, 2015

Record last verified: 2015-07

Locations