The Mechanisms and Significances of Synergistic Effects of Mycophenolic Acid and LPS on IL-1β Secretion by Mononuclear
1 other identifier
observational
10
1 country
1
Brief Summary
To determine the mechanisms and significances of synergistic effects of mycophenolic acid and LPS on IL-1β secretion by mononuclear
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for all trials
Started Apr 2015
Shorter than P25 for all trials
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
April 1, 2015
CompletedFirst Submitted
Initial submission to the registry
April 25, 2015
CompletedFirst Posted
Study publicly available on registry
May 6, 2015
CompletedPrimary Completion
Last participant's last visit for primary outcome
November 1, 2015
CompletedStudy Completion
Last participant's last visit for all outcomes
March 1, 2016
CompletedMay 6, 2015
April 1, 2015
7 months
April 25, 2015
April 30, 2015
Conditions
Outcome Measures
Primary Outcomes (1)
mean ± SD of percentage of IL-1β production induced by Lipopolysaccharide in associate with MPA in monocytes.
six months
Interventions
Monocytes were co-cultured with LPS and/or MPA.After 12 hours, the supernatants were harvested and stored at -80°C for ELISA.
Eligibility Criteria
Healthy Volunteers,age 18-60.
You may qualify if:
- Healthy Volunteers，age 18-60.
You may not qualify if:
- Don't agree with informed consent
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
Third Affiliated Hospital of Southern Medical University
Guangzhou, Guangdong, 510630, China
Biospecimen
Peripheral blood was obtained from healthy donors,mononuclear cells (PBMCs) were isolated using Ficoll density gradient centrifugation, and LPS (1 μg/ml) and/or MPA were added. After 12 hours, the supernatants were harvested and stored at -80°C for ELISA.
MeSH Terms
Conditions
Interventions
Condition Hierarchy (Ancestors)
Intervention Hierarchy (Ancestors)
Study Officials
- STUDY DIRECTOR
erwei Sun, doctor
Third Affiliated Hospital of Southern Medical University
Central Study Contacts
Study Design
- Study Type
- observational
- Observational Model
- CASE ONLY
- Time Perspective
- CROSS SECTIONAL
- Sponsor Type
- OTHER GOV
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Dr.Xuechan Huang
Study Record Dates
First Submitted
April 25, 2015
First Posted
May 6, 2015
Study Start
April 1, 2015
Primary Completion
November 1, 2015
Study Completion
March 1, 2016
Last Updated
May 6, 2015
Record last verified: 2015-04