NCT02425241

Brief Summary

This is a clinical proof-of-concept (PoC) study of DNA vaccination after SAP depletion. The investigators will measure the immune responses to DNA vaccination against HIV-1 in healthy adult male volunteers, comparing a group in whom SAP has been completely depleted at the time of DNA vaccination and a control group vaccinated without SAP depletion.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
41

participants targeted

Target at P50-P75 for phase_1 hiv

Timeline
Completed

Started Oct 2013

Longer than P75 for phase_1 hiv

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

April 29, 2013

Completed
5 months until next milestone

Study Start

First participant enrolled

October 1, 2013

Completed
1.6 years until next milestone

First Posted

Study publicly available on registry

April 23, 2015

Completed
9 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 1, 2016

Completed
7 months until next milestone

Study Completion

Last participant's last visit for all outcomes

September 1, 2016

Completed
Last Updated

March 26, 2020

Status Verified

March 1, 2020

Enrollment Period

2.3 years

First QC Date

April 29, 2013

Last Update Submit

March 25, 2020

Conditions

Keywords

VaccineDNAHIV-1Serum amyloid P component (SAP)Clinical trialVaccinationProphylaxisAdult male

Outcome Measures

Primary Outcomes (2)

  • vaccine safety (Proportion of volunteers who develop a grade 3 or grade 4 local reaction/ grade 3 or 4 systemic reaction)

    Proportion of volunteers who develop a grade 3 or grade 4 local reaction. Proportion of volunteers who develop a grade 3 or grade 4 systemic reaction.

    20 weeks

  • vaccine immunogenicity (T cell responses will be determined initially by interferon-gamma enzyme-linked immunospot assay)

    T cell responses will be determined initially by interferon-gamma enzyme-linked immunospot assay.

    20 weeks

Study Arms (2)

CPHPC

EXPERIMENTAL

CPHPC infusion over 26 hours to deplete SAP at weeks 0,4 and 8. pSG2.HIVconsv DNA vaccine 4 mg at weeks 0, 4 and 8 after 24 hours of CPHPC infusion. ChAdV63.HIVconsv booster vaccine 5 x 10\^10 vp at week 12. MVA.HIVconsv booster vaccine 2 x 10\^8 pfu at week 20

Biological: pSG2.HIVconsv DNA vaccineBiological: ChAdV63.HIVconsv booster vaccineBiological: MVA.HIVconsv booster vaccineDrug: CPHPC

0.9% w/v saline solution

PLACEBO COMPARATOR

Placebo (normal saline) infusion over 26 hours at weeks 0,4 and 8. pSG2.HIVconsv DNA vaccine 4 mg at weeks 0, 4 and 8 after 24 hours of placebo infusion. ChAdV63.HIVconsv booster vaccine 5 x 10\^10 vp at week 12. MVA.HIVconsv booster vaccine 2 x 10\^8 pfu at week 20

Biological: pSG2.HIVconsv DNA vaccineBiological: ChAdV63.HIVconsv booster vaccineBiological: MVA.HIVconsv booster vaccineOther: Placebo

Interventions

pSG2.HIVconsv DNA 4 mg at weeks 0, 4 and 8.

0.9% w/v saline solutionCPHPC

ChAdV63.HIVconsv 5 x 10\^10 vp at week 12.

0.9% w/v saline solutionCPHPC

MVA.HIVconsv 2 x 10\^8 pfu at week 20

0.9% w/v saline solutionCPHPC
CPHPCDRUG

40 mg CPHPC IV infusion for 26 hours at weeks 0, 4 and 8 during which pSG2.HIVconsv is administered after 24 hours.

Also known as: (R)-1-[6-[(R)-2-carboxy-pyrrolidin-1-yl]-6-oxohexanoyl] pyrrolidine-2-carboxylic acid
CPHPC
PlaceboOTHER

Placebo IV infusion for 26 hours at weeks 0, 4 and 8 during which pSG2.HIVconsv is administered after 24 hours.

Also known as: 0.9% saline
0.9% w/v saline solution

Eligibility Criteria

Age18 Years - 50 Years
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy males, as assessed by a medical history, physical examination and laboratory tests.
  • Aged at least 18 years on the day of screening and no greater than 50 years on the day of the first vaccination.
  • Willing to comply with the requirements of the protocol and available for follow-up for the planned duration of the study.
  • In the opinion of the Chief Investigator (CI) or designee, the volunteer has understood the information provided and is able to provide written informed consent, which includes compliance with the requirements and restrictions listed in the consent form.
  • Willing to undergo HIV-1 testing, HIV-1 counselling and receive HIV-1 test results.
  • If heterosexually active male; willing to use an effective method of contraception from the day of the first vaccination until six weeks after the last vaccination.
  • Willing to forgo donating blood during the study.

You may not qualify if:

  • None.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

National Amyloidosis Centre

London, England, NW3 2PF, United Kingdom

Location

Related Publications (1)

  • Borthwick NJ, Lane T, Moyo N, Crook A, Shim JM, Baines I, Wee EG, Hawkins PN, Gillmore JD, Hanke T, Pepys MB. Randomized phase I trial HIV-CORE 003: Depletion of serum amyloid P component and immunogenicity of DNA vaccination against HIV-1. PLoS One. 2018 May 17;13(5):e0197299. doi: 10.1371/journal.pone.0197299. eCollection 2018.

MeSH Terms

Interventions

Saline Solution

Intervention Hierarchy (Ancestors)

Crystalloid SolutionsIsotonic SolutionsSolutionsPharmaceutical Preparations

Study Officials

  • Julian D Gillmore, MBBS

    University College, London

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

April 29, 2013

First Posted

April 23, 2015

Study Start

October 1, 2013

Primary Completion

February 1, 2016

Study Completion

September 1, 2016

Last Updated

March 26, 2020

Record last verified: 2020-03

Locations