Serological Evaluation of 77A/G and 11A/12A Gene of MMP-13 in Periodontitis Affected and Healthy Individuals

NCT02423304 | Unknown DMC

• 1 other identifier: Divya Aiswairya thesis
• Study Type: observational
• Target: 100
• Locations: 0 countries
• Sites: N/A

Brief Summary

50 periodontitis 50 healthy individuals serological evaluation will done to see association of 77A/G AND 11A/12A gene polymorphisms of MMP-13

Conditions

Generalized Adult Periodontitis

Outcome Measures

Primary Outcomes (1)

• to evaluate the association of 77A/G AND 11A/12A gene polymorphism of mmp-13 in periodontitis effected and healthy individuals

  collect DNA samples from serum and evaluate for gene polymorphism

  6 months

Study Arms (2)

test

50 periodontitis patients • Patients will be categorized by Periodontal Disease Index (PDI) by P. Ramfjord(1959) blood samples collected from all patients serum separated and evaluated for77A/G AND 11A/12A associated gene polymorphism of Matrix Metallo Proteinase( MMP)-13 in all the patients. studies showing that there is co relation of these genes with increase inflammation.

Genetic: blood samples collected from all patients

control

50 periodontally healthy individuals blood samples collected from all patients serum separated and evaluated for77A/G AND 11A/12A associated gene polymorphism of Matrix Metallo Proteinase (MMP)-13 in all the patients

Genetic: blood samples collected from all patients

Interventions

blood samples collected from all patients GENETIC

blood samples collected from brachial pluxes

control; test

Eligibility Criteria

• Age: 30 Years - 55 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

* The study will be conducted on 100 individuals * 50 chronic periodontitis and 50 healthy individuals will be recruited from the Out Patient Department of Periodontics in the study.

You may qualify if:

• Patients with chronic periodontitis classified according to American Academy of Periodontology ( AAP) 1999
• Subjects aged between 30-55 years
• Control group Systemically and periodontally healthy individuals of same age group

You may not qualify if:

• Present and past Smokers
• Presence of any systemic diseases.
• Oral diseases other than Periodontitis
• With a previous history of periodontal treatment in the past 6 months and subjects under any medications.
• Family history of any diagnosed genetic disorders.

Sponsors & Collaborators

• Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre: lead
• Research Institute for Genetic and Human Therapy: collaborator

Related Publications (4)

• Pirhan D, Atilla G, Emingil G, Tervahartiala T, Sorsa T, Berdeli A. MMP-13 promoter polymorphisms in patients with chronic periodontitis: effects on GCF MMP-13 levels and outcome of periodontal therapy. J Clin Periodontol. 2009 Jun;36(6):474-81. doi: 10.1111/j.1600-051X.2009.01399.x. Epub 2009 Apr 22.

  PMID: 19453573 BACKGROUND

• Uitto VJ, Airola K, Vaalamo M, Johansson N, Putnins EE, Firth JD, Salonen J, Lopez-Otin C, Saarialho-Kere U, Kahari VM. Collagenase-3 (matrix metalloproteinase-13) expression is induced in oral mucosal epithelium during chronic inflammation. Am J Pathol. 1998 Jun;152(6):1489-99.

  PMID: 9626053 RESULT

• Kiili M, Cox SW, Chen HY, Wahlgren J, Maisi P, Eley BM, Salo T, Sorsa T. Collagenase-2 (MMP-8) and collagenase-3 (MMP-13) in adult periodontitis: molecular forms and levels in gingival crevicular fluid and immunolocalisation in gingival tissue. J Clin Periodontol. 2002 Mar;29(3):224-32. doi: 10.1034/j.1600-051x.2002.290308.x.

  PMID: 11940142 RESULT

• Hernandez Rios M, Sorsa T, Obregon F, Tervahartiala T, Valenzuela MA, Pozo P, Dutzan N, Lesaffre E, Molas M, Gamonal J. Proteolytic roles of matrix metalloproteinase (MMP)-13 during progression of chronic periodontitis: initial evidence for MMP-13/MMP-9 activation cascade. J Clin Periodontol. 2009 Dec;36(12):1011-7. doi: 10.1111/j.1600-051X.2009.01488.x.

  PMID: 19929954 RESULT

Biospecimen

Retention: SAMPLES WITH DNA

blood serum

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: dr.M.Divya Aiswairya

Study Record Dates

• First Submitted: April 17, 2015
• First Posted: April 22, 2015
• Study Start: May 1, 2015
• Primary Completion: May 1, 2016
• Study Completion: June 1, 2016
• Last Updated: April 22, 2015

Record last verified: 2015-04