NCT02414789

Brief Summary

Test and evaluate a new diagnostic method (SRM-MS/MS: Selected Reaction Monitoring- Mass spectrometry) for Lyme Borreliosis on human skin biopsies. Patients included are those with the early skin manifestation (erythema migrans). This new proteomic method will be compared to the two existing method: culture of Borrelia and PCR detection of Borrelia (DNA detection of the bacteria).

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
84

participants targeted

Target at P50-P75 for not_applicable

Timeline
Completed

Started Aug 2015

Longer than P75 for not_applicable

Geographic Reach
1 country

7 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

April 8, 2015

Completed
5 days until next milestone

First Posted

Study publicly available on registry

April 13, 2015

Completed
4 months until next milestone

Study Start

First participant enrolled

August 6, 2015

Completed
8.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 1, 2024

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

February 1, 2024

Completed
Last Updated

October 1, 2025

Status Verified

September 1, 2025

Enrollment Period

8.5 years

First QC Date

April 8, 2015

Last Update Submit

September 26, 2025

Conditions

Keywords

erythema migransLymeearly diagnosisskin biomarkersproteomics diagnosticmass spectrometry

Outcome Measures

Primary Outcomes (1)

  • Compare the sensitivity (percentage of positive tests) of the SRM / MS-MS to techniques of PCR and culture Borrelia

    percentage of positive tests with all three techniques (SRM/MS-MS ; PCR ; culture)

    One year study

Study Arms (1)

SRM / MS-MS

EXPERIMENTAL
Procedure: Skin biopsies

Interventions

Skin biopsiesPROCEDURE

Skin biopsies of patients with Lyme disease (erythema migrans) infected with Borrelia burgdorferi sensu lato will be analyzed by three diagnostic techniques : molecular technique (PCR), biological technique (culture of the bacteria) and proteomics technique (SRM/ MS-MS)

SRM / MS-MS

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Erythema migrant as criteria EUCALB or atypical form associated to tick's sting
  • Patient having signed a consent
  • Patient affiliated to a social security scheme

You may not qualify if:

  • Prior treatment with antibiotics
  • Erythema migrans on the face
  • Bleeding disorders
  • Anticoagulant treatment
  • Inability to give clear information (subject in an emergency situation, understanding of the topic difficulty, ...)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (7)

Centre Hospitalo-Universitaire Jean Minjoz

Besançon, France

Location

Centre Hospitalier William Morey

Chalon-sur-Saône, France

Location

Hôpital Pasteur

Colmar, France

Location

Hôpital E. Muller

Mulhouse, France

Location

Hôpital Robert Debré

Reims, France

Location

Hôpitaux Universitaires de Strasbourg

Strasbourg, 67091, France

Location

Hôpital Bel Air

Thionville, France

Location

Related Publications (1)

  • Cantero P, Ehret-Sabatier L, Lenormand C, Hansmann Y, Sauleau E, Zilliox L, Westermann B, Jaulhac B, Mutter D, Barthel C, Perdu-Alloy P, Martinot M, Lipsker D, Boulanger N. Detection of Borrelia burgdorferi sensu lato by proteomics: a complementary diagnosis tool on erythema migrans biopsies. Clin Microbiol Infect. 2025 Jan;31(1):78-86. doi: 10.1016/j.cmi.2024.10.014. Epub 2024 Oct 23.

    PMID: 39454756BACKGROUND

MeSH Terms

Conditions

Glossitis, Benign MigratoryDisease

Condition Hierarchy (Ancestors)

GlossitisTongue DiseasesMouth DiseasesStomatognathic DiseasesPathologic ProcessesPathological Conditions, Signs and Symptoms

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

April 8, 2015

First Posted

April 13, 2015

Study Start

August 6, 2015

Primary Completion

February 1, 2024

Study Completion

February 1, 2024

Last Updated

October 1, 2025

Record last verified: 2025-09

Locations