Investigate the Role of microRNA in Chronic Rhinosinusitis From DC-Th Axis

NCT02325596 | Unknown

• 1 other identifier: NSFC-81271061
• Study Type: observational
• Target: 70
• Locations: 1 country
• Sites: 1

Brief Summary

This study aimed to evaluate the upstream events of Th17/Treg imbalance in CRS and their immune regulatory factors. Then the investigators aimed to explore the regulatory role of miRNA on DC-Th axis and its dysfunction in the pathogenesis of CRS, in order to determine the miRNA expression profile in CRS and clarify the role of miRNA in the pathogenesis of CRS by regulating the DC-Th axis.

Conditions

Rhinosinusitis

Keywords

Chronic rhinosinusitis; microRNA; dendritic cell; helper T cell

Outcome Measures

Primary Outcomes (1)

• Abnormal microRNA in the Chronic Rhinosinusitis are found by gene chip and qPCR.

  6 to 24 months post procedure

Study Arms (4)

control

Patients with only nasal septum deviation

Genetic: miRNA mimics or inhibitors

CRS sNP

Chronic Rhinosinusitis patients without nasal polyps

Genetic: miRNA mimics or inhibitors

atopic CRS wNP

Chronic Rhinosinusitis patients with allergic constitution and nasal polyps

Genetic: miRNA mimics or inhibitors

non-atopic CRS wNP

Chronic Rhinosinusitis patients with nasal polyps but not allergic constitution

Genetic: miRNA mimics or inhibitors

Interventions

miRNA mimics or inhibitors GENETIC

DCs are transfected by the miRNA mimics or inhibitors after they are separated from peripheral blood mononuclear cells in CRS patients

CRS sNP; atopic CRS wNP; control; non-atopic CRS wNP

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Participants will be selected from the department of endoscopic sinus surgery in the First Affiliated Hospital of Chongqing Medical University.

You may qualify if:

• CRS diagnosis is accord with diagnostic criteria of The European nasal sinusitis, nasal polyps guidelines (EPOS 2012) .
• Selected subjects are stopped using systemic or topical corticosteroids in one month before experiment.
• All the patients have sinus CT and nasal endoscopy and allergen Skin Prick Test in preoperative.
• Aged from 18 to 75 years old.
• Woman or man.

You may not qualify if:

• Choanal polyp, fungal nasal sinusitis, cystic fibrosis.
• Acute upper respiratory tract infection and other diseases associated with the body.

Sponsors & Collaborators

• First Affiliated Hospital of Chongqing Medical University: lead

Study Sites (1)

The First Affiliated Hospital of Chongqing Medical University

Chongqing, Chongqing Municipality, 400016, China

Location

Related Publications (1)

• Ma ZX, Tan X, Shen Y, Ke X, Yang YC, He XB, Wang ZH, Dai YB, Hong SL, Hu GH. MicroRNA expression profile of mature dendritic cell in chronic rhinosinusitis. Inflamm Res. 2015 Nov;64(11):885-93. doi: 10.1007/s00011-015-0870-5. Epub 2015 Sep 4.

  PMID: 26337346 DERIVED

MeSH Terms

Conditions

Rhinosinusitis

Condition Hierarchy (Ancestors)

Rhinitis; Respiratory Tract Infections; Infections; Sinusitis; Paranasal Sinus Diseases; Nose Diseases; Respiratory Tract Diseases; Otorhinolaryngologic Diseases

Study Officials

• Guo-hua Hu HG Guo-hua Hu, MD

  First Affiliated Hospital of Chongqing Medical University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Investigate the Role of microRNA in Chronic Rhinosinusitis From DC-Th Axis

Study Record Dates

• First Submitted: December 15, 2014
• First Posted: December 25, 2014
• Study Start: July 1, 2014
• Primary Completion: July 1, 2015
• Study Completion: December 1, 2016
• Last Updated: December 25, 2014

Record last verified: 2014-12

Locations

CN (1)