NCT02239614

Brief Summary

The potential synergistic effect of administering 2 dengue vaccine candidates that were previously shown to be safe and immunogenic in humans will be evaluated in this study. A prime-boost study of tetravalent dengue virus purified inactivated vaccine (TDENV-PIV) with alum and tetravalent dengue live attenuated virus (TDENV-LAV) vaccine Formulation 17 (F17) will gather data to help better understand the human immune response to dengue vaccination and infection.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
80

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started Dec 2014

Typical duration for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

September 10, 2014

Completed
5 days until next milestone

First Posted

Study publicly available on registry

September 15, 2014

Completed
3 months until next milestone

Study Start

First participant enrolled

December 1, 2014

Completed
1.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 17, 2016

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

February 17, 2017

Completed
Last Updated

August 15, 2018

Status Verified

August 1, 2018

Enrollment Period

1.2 years

First QC Date

September 10, 2014

Last Update Submit

August 14, 2018

Conditions

Keywords

dengue feverdengue

Outcome Measures

Primary Outcomes (6)

  • Number of solicited adverse events

    21 days after each vaccination

  • Number of unsolicited adverse events

    28 days after each vaccination

  • Number of hematological and biochemistry abnormalities

    7 and 28 days and each vaccination

  • Number of serious adverse events

    Day 208 or day 360

  • Number of potential immune-mediated diseases

    Day 208 or day 360

  • Number of medically attended adverse events

    Day 208 or day 360

Secondary Outcomes (1)

  • Microneutralizing (MN) dengue antibody titers

    Up to 1 year

Study Arms (4)

Group 1: LAV (T=0), PIV (T=28)

EXPERIMENTAL

Tetravalent live-attenuated dengue virus vaccine formulation 17 (TDENV-LAV) reconstituted with 0.7 mL of water-for-injection; administered subcutaneously on day 0 of the study. Tetravalent dengue purified inactivated vaccine 4 μg/serotype with alum adjuvant (TDENV-PIV 4 µg + alum adjuvant) administered intramuscularly on day 28 of the study.

Biological: TDENV-LAVBiological: TDENV-PIV 4 µg + alum adjuvant

Group 2: PIV (T=0), LAV (T=28)

EXPERIMENTAL

Tetravalent dengue purified inactivated vaccine 4 μg/serotype with alum adjuvant (TDENV-PIV 4 µg + alum adjuvant) administered intramuscularly on day 0 of the study. Tetravalent live-attenuated dengue virus vaccine formulation 17 (TDENV-LAV) reconstituted with 0.7 mL of water-for-injection; administered subcutaneously on day 28 of the study.

Biological: TDENV-LAVBiological: TDENV-PIV 4 µg + alum adjuvant

Group 3: LAV (T=0), PIV (T=180)

EXPERIMENTAL

Tetravalent live-attenuated dengue virus vaccine formulation 17 (TDENV-LAV) reconstituted with 0.7 mL of water-for-injection; administered subcutaneously on day 0 of the study. Tetravalent dengue purified inactivated vaccine 4 μg/serotype with alum adjuvant (TDENV-PIV 4 µg + alum adjuvant) administered intramuscularly on day 180 of the study.

Biological: TDENV-LAVBiological: TDENV-PIV 4 µg + alum adjuvant

Group 4: PIV (T=0), LAV (T=180)

EXPERIMENTAL

Tetravalent dengue purified inactivated vaccine 4 μg/serotype with alum adjuvant (TDENV-PIV 4 µg + alum adjuvant) administered intramuscularly on day 0 of the study. Tetravalent live-attenuated dengue virus vaccine formulation 17 (TDENV-LAV) reconstituted with 0.7 mL of water-for-injection; administered subcutaneously on day 180 of the study.

Biological: TDENV-LAVBiological: TDENV-PIV 4 µg + alum adjuvant

Interventions

TDENV-LAVBIOLOGICAL

0.5 mL of the post-transfection LAV F17 vaccine

Also known as: TDENV-LAV F17, Tetravalent dengue live attenuated virus formulation 17
Group 1: LAV (T=0), PIV (T=28)Group 2: PIV (T=0), LAV (T=28)Group 3: LAV (T=0), PIV (T=180)Group 4: PIV (T=0), LAV (T=180)

0.5 mL of DENV serotypes 1-4 (4 µg / serotype) in alum adjuvant

Also known as: Tetravalent dengue virus purified inactivated vaccine with alum adjuvant
Group 1: LAV (T=0), PIV (T=28)Group 2: PIV (T=0), LAV (T=28)Group 3: LAV (T=0), PIV (T=180)Group 4: PIV (T=0), LAV (T=180)

Eligibility Criteria

Age18 Years - 49 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Male or female between 18 and 49 years of age (inclusive) at the time of consent
  • Able to provide written informed consent
  • Healthy as established by medical history and clinical examination before entering into the study
  • Able and willing to comply with the requirements of the protocol (eg, document events in memory aid, return for follow-up visits, etc.)
  • Female subject of non-childbearing potential (non-childbearing potential is defined as having either a current tubal ligation at least 3 months prior to enrollment or a history of a hysterectomy, ovariectomy, or is post-menopause)
  • Female subject is not breastfeeding and agrees not to breastfeed for 3 months after last vaccination
  • Female subject of childbearing potential may be enrolled in the study, if the subject has:
  • Practiced adequate contraception for 30 days prior to vaccinations, and
  • A negative urine pregnancy test on each day of vaccination, and
  • Agreed to continue adequate contraception until 3 months after completion of the vaccination series.

You may not qualify if:

  • Use of any investigational or non-registered product (drug or vaccine) other than the study vaccines during the period starting 30 days preceding the first dose of study vaccine and/or planned use during the study period
  • Chronic administration (defined as more than 14 days in total) of immunosuppressants or other immune-modifying drugs during the period starting 180 days prior to the first vaccine dose
  • For corticosteroids, this will mean prednisone ≥ 20 mg/d or equivalent
  • Inhaled and topical steroids are allowed
  • Planned administration or administration of a vaccine/product not foreseen by the study protocol during the period starting 14 days before or after each scheduled dose of an investigational product
  • Planned administration of any flavivirus vaccine for the entire study duration
  • Concurrently participating in another clinical study, at any time during the study period, in which the subject has been or will be exposed to an investigational or a non-investigational product (pharmaceutical product or device)
  • Any confirmed or suspected immunosuppressive or immunodeficient condition, based on medical history and physical examination (no laboratory testing required)
  • Family history of congenital or hereditary immunodeficiency
  • History of, or current, auto-immune disease
  • History of any reaction or hypersensitivity likely to be exacerbated by any component of the vaccine or related to a study procedure
  • Major congenital defects or serious chronic illness
  • History of any neurological disorders or seizures. (except for a childhood febrile seizures)
  • Acute disease and/or fever (oral body temperature ≥ 100.4°F/38.0°C) at the time of enrollment (a subject with a minor illness, ie, mild diarrhea, mild upper respiratory infection, etc, without fever, may be enrolled at the discretion of the investigator)
  • Acute or chronic, clinically significant pulmonary, cardiovascular, hepatic, or renal functional abnormality, as determined by physical examination or laboratory screening tests
  • +10 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Clinical Trials Center, Walter Reed Army Institute of Research (CTC, WRAIR)

Silver Spring, Maryland, 20910, United States

Location

Related Publications (1)

  • Lin L, Koren MA, Paolino KM, Eckels KH, De La Barrera R, Friberg H, Currier JR, Gromowski GD, Aronson NE, Keiser PB, Sklar MJ, Sondergaard EL, Jasper LE, Endy TP, Jarman RG, Thomas SJ. Immunogenicity of a Live-Attenuated Dengue Vaccine Using a Heterologous Prime-Boost Strategy in a Phase 1 Randomized Clinical Trial. J Infect Dis. 2021 May 28;223(10):1707-1716. doi: 10.1093/infdis/jiaa603.

MeSH Terms

Conditions

Dengue

Interventions

Aluminum Hydroxide

Condition Hierarchy (Ancestors)

Mosquito-Borne DiseasesVector Borne DiseasesInfectionsArbovirus InfectionsVirus DiseasesFlavivirus InfectionsFlaviviridae InfectionsRNA Virus InfectionsHemorrhagic Fevers, Viral

Intervention Hierarchy (Ancestors)

HydroxidesAlkaliesInorganic ChemicalsAluminum CompoundsAnionsIonsElectrolytes

Study Officials

  • MAJ Leyi Lin, MD

    Walter Reed Army Institute of Research (WRAIR)

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
FED
Responsible Party
SPONSOR

Study Record Dates

First Submitted

September 10, 2014

First Posted

September 15, 2014

Study Start

December 1, 2014

Primary Completion

February 17, 2016

Study Completion

February 17, 2017

Last Updated

August 15, 2018

Record last verified: 2018-08

Locations